Gold nanostructures modified carbon-based electrode enhanced with methylene blue for point-of-care COVID-19 tests using isothermal amplification

Kim, Hyo Eun; Schuck, Ariadna; Park, Hyeonseek; Huh, Hee Jae; Kang, Minhee; Kim, Yong−Sang

doi:10.1016/j.talanta.2023.124841

Cited by 9 publications

(5 citation statements)

References 44 publications

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“…LAMP has shown significant promise as a screening method, especially in addressing pandemic situations and infectious diseases in resource-limited settings, such as SARS-CoV-2 (Huang et al 2020 ; Kim et al 2023 , 2019 ), malaria (Ponce et al 2017 ), human visceral leishmaniasis (de Avelar et al 2019 ), and Zika virus (Silva et al 2019 ). However, some challenges remain before it can replace conventional qPCR as a standard test.…”

Section: Discussionmentioning

confidence: 99%

Loop-mediated isothermal amplification assay for screening congenital cytomegalovirus infection in newborns

Park,

Kim,

Shin

et al. 2023

Appl Microbiol Biotechnol

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Congenital cytomegalovirus (CMV) infection is a common cause of sensorineural hearing loss and neurodevelopmental impairment in newborns. However, congenital CMV infection cannot be diagnosed using samples collected more than 3 weeks after birth because testing after this time cannot distinguish between congenital infection and postnatal infection. Herein, we developed a robust loop-mediated isothermal amplification (LAMP) assay for the large-scale screening of newborns for congenital CMV infection. In contrast to conventional quantitative polymerase chain reaction (qPCR), which detects CMV within a dynamic range of 1.0 × 106 to 1.0 × 102 copies/μL, our quantitative LAMP assay (qLAMP) detects CMV within a dynamic range of 1.1 × 108 to 1.1 × 103 copies/μL. Moreover, the turnaround time for obtaining results following DNA extraction is 90 min in qPCR but only 15 min in qLamp. The colorimetric LAMP assay can also detect CMV down to 1.1 × 103 copies/μL within 30 min, irrespective of the type of heat source. Our LAMP assay can be utilized in central laboratories as an alternative to conventional qPCR for quantitative CMV detection, or for point-of-care testing in low-resource environments, such as developing countries, via colorimetric naked-eye detection. Key points • LAMP assay enables large-scale screening of newborns for congenital CMV infection. • LAMP allows colorimetric or quantitative detection of congenital CMV infection. • LAMP assay can be used as a point-of-care testing tool in low-resource environments.

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Section: Discussionmentioning

confidence: 99%

Loop-mediated isothermal amplification assay for screening congenital cytomegalovirus infection in newborns

Park,

Kim,

Shin

et al. 2023

Appl Microbiol Biotechnol

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“…The latter techniques (e.g., chemiluminescent methods, ELISA, antibody tests) strongly depend on the kinetics of SARS-CoV-2 antibody, and thus the optimization of the tests and interpretation of the results can be challenging. Additional detection methods for SARS-CoV-2 virus include optical biosensors [5], electrochemical [6][7][8], FET biosensor [9], colorimetric detection [10], and others [11][12][13].…”

Section: Introductionmentioning

confidence: 99%

Machine Learning for COVID-19 Determination Using Surface-Enhanced Raman Spectroscopy

Szymborski,

Berus,

Nowicka

et al. 2024

Biomedicines

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The rapid, low cost, and efficient detection of SARS-CoV-2 virus infection, especially in clinical samples, remains a major challenge. A promising solution to this problem is the combination of a spectroscopic technique: surface-enhanced Raman spectroscopy (SERS) with advanced chemometrics based on machine learning (ML) algorithms. In the present study, we conducted SERS investigations of saliva and nasopharyngeal swabs taken from a cohort of patients (saliva: 175; nasopharyngeal swabs: 114). Obtained SERS spectra were analyzed using a range of classifiers in which random forest (RF) achieved the best results, e.g., for saliva, the precision and recall equals 94.0% and 88.9%, respectively. The results demonstrate that even with a relatively small number of clinical samples, the combination of SERS and shallow machine learning can be used to identify SARS-CoV-2 virus in clinical practice.

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“…Moreover, LAMP requires a set of four specific primers that recognize six distinct sequences on the target DNA, limiting the simultaneous detection of multiple genes in a single reaction. Therefore, the combination of LAMP with biosensors maximizing multiplex capabilities has been extensively investigated [8,14,17]. For example, electrochemical sensors with multiple electrodes that measure the redox activity of electroactive molecules resulting from their interaction with LAMP products allow for highly sensitive detection.…”

Section: Introductionmentioning

confidence: 99%

“…For example, electrochemical sensors with multiple electrodes that measure the redox activity of electroactive molecules resulting from their interaction with LAMP products allow for highly sensitive detection. This approach involves immobilizing a probe onto a working electrode coupled with a redox reporter, and the electrochemical signals are then meticulously monitored using techniques that include linear-sweep [18], square-wave [19], and differential-pulse voltammetry [17,20]. Additionally, label-free electrical sensing using field-effect transistors (FETs) has been employed to detect pH changes during amplification reactions with various geometries and gating methods [21,22].…”

Section: Introductionmentioning

confidence: 99%

Dual-Mode Graphene Field-Effect Transistor Biosensor with Isothermal Nucleic Acid Amplification

Kim,

Schuck,

Park

et al. 2024

Biosensors

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Despite a substantial increase in testing facilities during the pandemic, access remains a major obstacle, particularly in low-resource and remote areas. This constraint emphasizes the need for high-throughput potential point-of-care diagnostic tools in environments with limited resources. Loop-mediated isothermal amplification (LAMP) is a promising technique, but improvements in sensitivity are needed for accurate detection, especially in scenarios where the virus is present in low quantities. To achieve this objective, we present a highly sensitive detection approach of a dual-mode graphene-based field-effect transistor (G-FET) biosensor with LAMP. The G-FET biosensor, which has a transparent graphene microelectrode array on a glass substrate, detects LAMP products in less than 30 min using both observable color changes and Dirac point voltage measurements, even in samples with low viral concentrations. This dual-mode G-FET biosensor emerges as a potential alternative to conventional RT-PCR for severe acute respiratory syndrome-associated coronavirus (SARS-CoV)-2 detection or point-of-care testing, particularly in resource-constrained scenarios such as developing countries. Moreover, its capacity for colorimetric detection with the naked eye enhances its applicability in diverse settings.

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Gold nanostructures modified carbon-based electrode enhanced with methylene blue for point-of-care COVID-19 tests using isothermal amplification

Cited by 9 publications

References 44 publications

Loop-mediated isothermal amplification assay for screening congenital cytomegalovirus infection in newborns

Loop-mediated isothermal amplification assay for screening congenital cytomegalovirus infection in newborns

Machine Learning for COVID-19 Determination Using Surface-Enhanced Raman Spectroscopy

Dual-Mode Graphene Field-Effect Transistor Biosensor with Isothermal Nucleic Acid Amplification

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