Goldfish, Carassius auratus, as an infection model for studying the pathogenesis of Edwardsiella piscicida

Choe, Yun-Jeong; Yu, Jinghua; Park, Jun-Mo; Park, Dongchul; Oh, Jun-Hyok; Kim, Suhkmann; Moon, Ki Hwan; Kang, Ho Young

doi:10.1007/s11259-017-9700-2

Cited by 11 publications

(8 citation statements)

References 33 publications

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“…Goldfish, Carassius auratus (Linnaeus, 1758), is a domesticated fish species originating from central Asia and China that has been introduced throughout the world. Goldfish belongs to the Cyprinidae family and is considered as an important fish model for research in endocrinology [1,2], developmental biology [3,4] or fish pathology [5]. Thanks to the recent availability of a whole genome sequence assembly [6], goldfish is also now becoming a key model species for studies on genomics and cyprinid genome evolution.…”

Section: Introductionmentioning

confidence: 99%

Sex chromosome and sex locus characterization in goldfish, Carassius auratus (Linnaeus, 1758)

et al. 2020

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Background: Goldfish is an important model for various areas of research, including neural development and behavior and a species of significant importance in aquaculture, especially as an ornamental species. It has a male heterogametic (XX/XY) sex determination system that relies on both genetic and environmental factors, with high temperatures being able to produce female-to-male sex reversal. Little, however, is currently known on the molecular basis of genetic sex determination in this important cyprinid model. Here we used sequencing approaches to better characterize sex determination and sex-chromosomes in an experimental strain of goldfish. Results: Our results confirmed that sex determination in goldfish is a mix of environmental and genetic factors and that its sex determination system is male heterogametic (XX/XY). Using reduced representation (RAD-seq) and whole genome (pool-seq) approaches, we characterized sex-linked polymorphisms and developed male specific genetic markers. These male specific markers were used to distinguish sex-reversed XX neomales from XY males and to demonstrate that XX female-to-male sex reversal could even occur at a relatively low rearing temperature (18°C), for which sex reversal has been previously shown to be close to zero. We also characterized a relatively large non-recombining region (~11.7 Mb) on goldfish linkage group 22 (LG22) that contained a high-density of male-biased genetic polymorphisms. This large LG22 region harbors 373 genes, including a single candidate as a potential master sex gene, i.e., the anti-Mullerian hormone gene (amh). However, no sex-linked polymorphisms were detected in the coding DNA sequence of the goldfish amh gene. Conclusions: These results show that our goldfish strain has a relatively large sex locus on LG22, which is likely the Y chromosome of this experimental population. The presence of a few XX males even at low temperature also suggests that other environmental factors in addition to temperature could trigger female-to-male sex reversal. Finally, we also developed sex-linked genetic markers, which will be important tools for future research on sex determination in our experimental goldfish population. However, additional work would be needed to explore whether this sex locus is conserved in other populations of goldfish.

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Section: Introductionmentioning

confidence: 99%

Sex chromosome and sex locus characterization in goldfish, Carassius auratus (Linnaeus, 1758)

et al. 2020

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“…Although the infection of E. piscicida in largemouth bass ( Micropterus salmoides ) Camus, Griffin, Armwood, & Soto (2019), zebrafish ( Brachydanio rerio ) (Wang et al, 2019) and goldfish ( Carassius auratus ) (Choe et al, 2017) have been reported, until now, molecular studies on the immune response to E. piscicida in the C. striata remain rare. In this study, we firstly analysed the liver transcriptome profile of the C. striata after E. piscicida infection and obtained the gene information.…”

Section: Resultsmentioning

confidence: 99%

“…Although the infection of E. piscicida in largemouth bass (Micropterus salmoides) Camus, Griffin, Armwood, & Soto (2019), zebrafish (Brachydanio rerio) (Wang et al, 2019) and goldfish (Carassius auratus) (Choe et al, 2017) have been reported, until now, molecular studies 3; Figure 7). Among them, TLR5 was up-regulated, which has been reported as membrane (TLR5M) and soluble (TLR5s) orthologs, and recognizes the flagellin protein component of bacterial flagella (Morimoto et al, 2019;Pietretti & Wiegertjes, 2014;Voogdt et al, 2018).…”

Section: Toll-like Receptor Signalling Pathwaymentioning

confidence: 99%

Comparative transcriptomic analysis of the liver of Centropristis striata in response to Edwardsiella piscicida infection

Xiang

et al. 2021

Aquaculture Research

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The Centropristis striata is an important economically marine fish and facing a serious threat from various pathogens. Edwardsiella piscicida is one of the fish pathogens that seriously infect cultured and wild fish species. To understand the molecular immune mechanisms underlying the response to E. piscicida, a comparative gene transcription analysis was performed through RNA‐Seq technology. The low‐quality sequences were excluded, and the high‐quality sequences were assembled into a total of 60,759 contigs with an average length of 988.95 bp. A gene ontology (GO) analysis was employed to classify 5744 genes according to three major functional categories: molecular function, cellular component and biological process. A total of 19,918 genes were successfully annotated into 26 functional categories according to the clusters of orthologous groups (COG) classification. Furthermore, 15,006 genes were classified into 332 KEGG pathways. The q‐value was introduced to estimate 5109 genes significantly differentially expressed which includes 1781 up‐regulated genes and 3328 down‐regulated genes. The differentially expressed genes were involved in the major immune‐related pathways which refer to the pathogen recognition, inflammation, Toll‐like receptor and chemokine signalling pathways. Moreover, 24,487 potential simple sequence repeats (SSRs) and 193,760 candidate single nucleotide polymorphisms (SNPs) were detected and identified in the C. striata liver transcriptome. This work not only provides a valuable resource for further understanding of the antibacterial molecular mechanism of C. striata but also contributes to facilitate future studies on gene expression and functional genomics.

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“…The pathogenicity of E. piscicida , part of which may have been misclassified as E. tarda in the past, might be underestimated compared to E. tarda (Bujan et al, 2018; Griffin et al, 2014). E. piscicida can infect a broad range of freshwater and seawater teleost fish species, like Pomoxis nigromaculatus (Griffin et al, 2019), Micropterus salmoides (Camus et al, 2019; Fogelson et al, 2016), Paralichthys olivaceus (Sugiura et al, 2018), Lates calcarifer (Loch et al, 2017), Carassius auratus (Choe et al, 2017), Taeniura meyeni (Camus et al, 2016), Ictalurus punctatus (Reichley et al, 2016) and Anguilla japonica (Tu & Kawai, 1998). In the meanwhile, edwardsiellosis caused great damage to the aquaculture industry, as the cumulative mortality of hybrid tilapia juveniles in Southeast Asia reached 40–50% (Dong et al, 2019); also, the mortality rate was up to 90–95% in adult turbot and 70–80% in juvenile ones (Wang et al, 2006); moreover, high mortality was observed in the eels with the cumulative mortality rate estimated to be >65% over a month (Jung et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

The pathway of Edwardsiella piscicida infecting Lateolabrax maculatus via the immersion bath

Hu,

Wang,

et al. 2023

Journal of Fish Diseases

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Edwardsiella piscicida, an infectious bacterium, causes great economic losses to the aquaculture industry. Immersion bath which is the closest way to how the fish infect bacterial pathogens in the natural environment is an effective route of artificial infection. In this study, the dynamic process of E. piscicida infection, in the spotted sea bass (Lateolabrax maculatus) was evaluated via the immersion bath. The results showed that soaking the spotted sea bass with 3 × 106 CFU mL−1 E. piscicida for 30 min could artificially induce edwardsiellosis. The higher culture temperature (28.5 ± 0.5°C) or the longer bath time (30 min) would lead to higher mortality of fish. E.piscicida first invaded the gill, then entered the blood circulation to infect the spleen and kidney, where it is colonized, and gradually multiplied in the liver and brain. Meanwhile, the fluorescence in situ hybridization showed that the localization of E. piscicida in the gill and foregut after the immersion challenge proceeded from the exterior to the interior. The invasion of pathogens triggers the immune response of fish and causes tissue damage to the host. The quantitative real‐time PCR results displayed an increase in the relative expression level of immune genes (NK‐lysin, LZM, IgM and IgD). Otherwise, the most notable histopathological changes of the infected spotted sea bass were multifocal necrosis. Findings in this study broaden our understanding of the infection conditions of E. piscicida and its pathogenicity to the spotted sea bass.

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Goldfish, Carassius auratus, as an infection model for studying the pathogenesis of Edwardsiella piscicida

Cited by 11 publications

References 33 publications

Sex chromosome and sex locus characterization in goldfish, Carassius auratus (Linnaeus, 1758)

Sex chromosome and sex locus characterization in goldfish, Carassius auratus (Linnaeus, 1758)

Comparative transcriptomic analysis of the liver of Centropristis striata in response to Edwardsiella piscicida infection

The pathway of Edwardsiella piscicida infecting Lateolabrax maculatus via the immersion bath

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