Goldfish Leptin-AI and Leptin-AII: Function and Central Mechanism in Feeding Control

Yan, Aifen; Chen, Ting; Chen, Shuang; Ren, Chun Hua; Hu, Chao; Cai, Yi; Liu, Fang; Tang, Dong Sheng

doi:10.3390/ijms17060783

Cited by 39 publications

(33 citation statements)

References 56 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In contrast, the amino acid sequence homology shared between leptin-AI and leptin-AII are much higher, e.g., 82.0% in common carp 25, and 79.5% in goldfish. Previously studies showed that the anorexigenic effects of goldfish leptin-AI and leptin-AII in were highly comparable 17. Our present study shows that the regulatory modules for goldfish leptin -AI and leptin -AII gene expression are also similar.…”

Section: Discussionsupporting

confidence: 72%

“…The anorexigenic effects of leptin have been described in teleost fish 17, 18 as well as in mammals 19. The nutritional status is shown highly correlated to leptin levels in fish but controversial between different species 4.…”

Section: Introductionmentioning

confidence: 99%

“…However, the leptin -B has not yet been reported in goldfish. Recombinant goldfish leptin-AI and leptin-AII protein were effective at inhibiting feeding behavior and reducing food consumption of goldfish via mediating of the transcript levels of some central appetite regulators 17. The liver is the metabolic center in fish, and it is also the major site for leptin expression in fish 25, 42.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Signal transduction mechanism for glucagon-induced leptin gene expression in goldfish liver

Yan¹,

Chen²,

Chen³

et al. 2016

Int. J. Biol. Sci.

Self Cite

View full text Add to dashboard Cite

Leptin is a peripheral satiety hormone that also plays important roles in energy homeostasis in vertebrates ranging from fish to mammals. In teleost fish, however, the regulatory mechanism for leptin gene expression still remains unclear. In this study, we found that glucagon, a key hormone in glucose homeostasis, was effective at elevating the leptin-AI and leptin-AII transcript levels in goldfish liver via both in vivo intraperitoneal injection and in vitro cells incubation approaches. The responses of leptin-AI and leptin-AII mRNA to glucagon treatment were highly comparable. In contrast, blockade of local glucagon action could reduce the basal and induced leptin-AI and leptin-AII mRNA expression. The stimulation of leptin levels by glucagon was caused by the activation of adenylate cyclase (AC)/cyclic-AMP (cAMP)/ protein kinase A (PKA), and probably cAMP response element-binding protein (CREB) cascades. Our study described the effect and signal transduction mechanism of glucagon on leptin gene expression in goldfish liver, and may also provide new insight into leptin as a mediator in the regulatory network of energy metabolism in the fish model.

show abstract

Section: Discussionsupporting

confidence: 72%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Signal transduction mechanism for glucagon-induced leptin gene expression in goldfish liver

Yan¹,

Chen²,

Chen³

et al. 2016

Int. J. Biol. Sci.

Self Cite

View full text Add to dashboard Cite

show abstract

“…In goldfish, leptin injections decrease feeding and locomotor behavior (Volkoff et al, 2003; de Pedro et al, 2006; Vivas et al, 2011; Tinoco et al, 2012) in part by stimulating anorexigenic sytems (e.g., CART, CCK, and POMC) and inhibiting orexigenic ones (e.g., orexin, NPY, AgRP) (Volkoff et al, 2003; Yan et al, 2016). Similarly, in rainbow trout (Salmoniforme), central leptin administration suppresses food intake and increases the hypothalamic expressions of CART and POMC (Gong et al, 2016).…”

Section: Hormones Involved In Food Intakementioning

confidence: 99%

The Neuroendocrine Regulation of Food Intake in Fish: A Review of Current Knowledge

2016

View full text Add to dashboard Cite

Fish are the most diversified group of vertebrates and, although progress has been made in the past years, only relatively few fish species have been examined to date, with regards to the endocrine regulation of feeding in fish. In fish, as in mammals, feeding behavior is ultimately regulated by central effectors within feeding centers of the brain, which receive and process information from endocrine signals from both brain and peripheral tissues. Although basic endocrine mechanisms regulating feeding appear to be conserved among vertebrates, major physiological differences between fish and mammals and the diversity of fish, in particular in regard to feeding habits, digestive tract anatomy and physiology, suggest the existence of fish- and species-specific regulating mechanisms. This review provides an overview of hormones known to regulate food intake in fish, emphasizing on major hormones and the main fish groups studied to date.

show abstract

“…On the other hand, leptin is a peripheral satiety factor that is mainly produced in the adipose tissue of mammals or the liver of fish [41,42]. In goldfish, leptin inhibits appetite by suppressing food intake and feeding behavior [43]. Leptins further regulate the expression of brain neuropeptides [43] and pituitary hormones [44] in goldfish.…”

Section: Discussionmentioning

confidence: 99%

Two Distinct C-Type Lysozymes in Goldfish: Molecular Characterization, Antimicrobial Potential, and Transcriptional Regulation in Response to Opposing Effects of Bacteria/Lipopolysaccharide and Dexamethasone/Leptin

Cui

Rao

et al. 2020

IJMS

Self Cite

View full text Add to dashboard Cite

Lysozymes are key antimicrobial peptides in the host innate immune system that protect against pathogen infection. In this study, the full-length cDNAs of two c-type lysozymes (gfLyz-C1 and gfLyz-C2) were cloned from goldfish (Carassius auratus). The structural domains, three-dimensional structures, and amino acid sequences of gfLyz-C1 and gfLyz-C2 were highly comparable, as the two proteins shared 89.7% sequence identity. The gfLyz-C1 and gfLyz-C2 recombinant proteins were generated in the insoluble fractions of an Escherichia coli system. Based on the results of lysoplate and turbidimetric assays, gfLyz-C1 and gfLyz-C2 showed broad-spectrum antimicrobial properties with high levels of activity against Micrococcus lysodeikticus, Vibrio parahemolyticus, and Edwardsiella tarda, and relatively low activity against E. coli. Both gfLyz-C1 and gfLyz-C2 mRNAs were mainly expressed in the trunk kidney and head kidney, and gfLyz-C1 was expressed at much higher levels than gfLyz-C2 in the corresponding tissues. The expression of the gfLyz-C1 and gfLyz-C2 transcripts in the trunk kidney and head kidney was induced in these tissues by challenge with heat-inactivated E. coli and lipopolysaccharides (LPS), and the transcriptional responses of gfLyz-C1 were more intense. In goldfish primary trunk kidney cells, the levels of the gfLyz-C1 and gfLyz-C2 transcripts were upregulated by heat-inactivated E. coli, V. parahemolyticus, and E. tarda, as well as LPS, and downregulated by treatment with dexamethasone and leptins. Overall, this study may provide new insights that will improve our understanding of the roles of c-type lysozymes in the innate immunity of cyprinid fish, including the structural and phylogenetic characteristics, antimicrobial effects, and regulatory mechanism.

show abstract

Goldfish Leptin-AI and Leptin-AII: Function and Central Mechanism in Feeding Control

Cited by 39 publications

References 56 publications

Signal transduction mechanism for glucagon-induced leptin gene expression in goldfish liver

Signal transduction mechanism for glucagon-induced leptin gene expression in goldfish liver

The Neuroendocrine Regulation of Food Intake in Fish: A Review of Current Knowledge

Two Distinct C-Type Lysozymes in Goldfish: Molecular Characterization, Antimicrobial Potential, and Transcriptional Regulation in Response to Opposing Effects of Bacteria/Lipopolysaccharide and Dexamethasone/Leptin

Contact Info

Product

Resources

About