Golgi-on-a-Chip for the Cell-Free Bio-Nanomanufacturing of Protein Therapeutics

Daniel, Susan; Aquino, Alicia; DeLisa, Matthew P.; Jaroentomeechai, Thapakorn; Liu, Han-Yuan; Manzer, Zachary A.; Pinnock, Ferra; Singh, Rohit

doi:10.1016/j.bpj.2018.11.025

Cited by 1 publication

(3 citation statements)

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“…Glycoproteins have immense therapeutic potential, though it demands extensive as well as costly purification steps, thus posing a challenge for its production (Daniel et al, 2019). These proteins are produced in a cellular compartment called Golgi apparatus where a protein undergoes series of reactions for its glycosylation.…”

Section: Potential Applications Of Cfps Systemsmentioning

confidence: 99%

“…These proteins are produced in a cellular compartment called Golgi apparatus where a protein undergoes series of reactions for its glycosylation. Few of the reasons for low yield of protein synthesis through cell-free system are competing reactions and formation of undesired side products (Daniel et al, 2019). However, a cell does not face the mentioned issues as it localizes a particular reaction at a particular place through compartmentalization.…”

Section: Potential Applications Of Cfps Systemsmentioning

confidence: 99%

“…However, a cell does not face the mentioned issues as it localizes a particular reaction at a particular place through compartmentalization. Learning from the cells, a Golgi-on-chip technique was devised to achieve compartmentalization in cell-free system for specific synthesis of glycoproteins (Daniel et al, 2019).…”

Section: Potential Applications Of Cfps Systemsmentioning

confidence: 99%

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Exploring the Potential of Cell-Free Protein Synthesis for Extending the Abilities of Biological Systems

Khambhati

Bhattacharjee

Gohil

et al. 2019

Front. Bioeng. Biotechnol.

101

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Cell-free protein synthesis (CFPS) system is a simple, rapid, and sensitive tool that is devoid of membrane-bound barriers, yet contains all the mandatory substrates, biomolecules, and machineries required for the synthesis of the desired proteins. It has the potential to overcome loopholes in the current in vivo production systems and is a promising tool in both basic and applied scientific research. It facilitates a simplified organization of desired experiments with a variety of reaction conditions, making CFPS a powerful tool in biological research. It has been used for the expansion of genetic code, assembly of viruses, and in metabolic engineering for production of toxic and complex proteins. Subsequently, CFPS systems have emerged as potent technology for high-throughput production of membrane proteins, enzymes, and therapeutics. The present review highlights the recent advances and uses of CFPS systems in biomedical, therapeutic, and biotechnological applications. Additionally, we highlight possible solutions to the potential biosafety issues that may be encountered while using CFPS technology.

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Section: Potential Applications Of Cfps Systemsmentioning

confidence: 99%

Section: Potential Applications Of Cfps Systemsmentioning

confidence: 99%

Section: Potential Applications Of Cfps Systemsmentioning

confidence: 99%

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