Göller Bölgesi Fasulye Üretim Alanlarında Fasulye Adi Mozayik ve Fasulye Adi Mozayik Nekroz Virüs’ü Enfeksiyonları

Abstract

Common bean, belonging to family Leguminosae (Fabaceae), has an important place in people nutrition for thousands of years. Although many viruses infect bean plants, economically most important ones are potyviruses. Among them, BCMV (Bean common mosaic virus) and BCMNV (Bean common mosaic necrosis virus) are the most common viruses in common bean production areas all over the world. During 2014-2015 growing season, total of 361 bean samples (275 leaf and 86 seed) were collected from several localities in the Lakes Region. According to the results of DAS-ELISA (Double Antibody Sandwich Enzyme Linked Immunusorbent Assay) test, percentages of the infections of BCMV and BCMNV in the leaf samples were 26.18% and 15.27%, respectively. In seed samples, BCMV and BCMNV infection rates were 2.32% and 6.97%, respectively. Percentages of the mixed infections of the viruses in the leaf samples were 25.45%, while that in the seed samples were. 55.81%. In the mechanically inoculation studies where two isolates (BCMV-I and BCMN-BT) were used, typical symptoms of the viruses were observed. In molecular studies, a 850 bp fragment of the CP (Coat Protein) gene was amplified by IC-RT-PCR (Immunocapture Reverse Transcription Polimerase Chain Reaction) using BCMV specific primer pairs, but amplification of the CP region of RNA genome of BCMNV could not be succeeded. This is first report of biological and serological methods in Lakes region.