Golovinomyces bolayi causes powdery mildew on Persian speedwell (Veronica persica) in Iran

Golmohammadi, Hadi; Torbati, Mohsen; Arzanlou, Mahdi

doi:10.1007/s13314-019-0351-8

Cited by 3 publications

(1 citation statement)

References 6 publications

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“…[ 10 , 32 ], according to the broad species concept for Golovinomyces [ 36 ]. However, some studies have reported G. orontii as the powdery mildew agent [ 9 , 25 ] and recognized that G. cichoracearum s. str. exclusively parasitizes plants in the tribe Cichorieae [ 37 ].…”

Section: Discussionmentioning

confidence: 99%

Taxonomy, Phylogeny, and Host Expansion of Powdery Mildew Fungi Parasitic to Veronica Species

Park,

Choi,

Shin

2024

Mycobiology

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Powdery mildew is a prevalent fungal disease that affects various Veronica spp. (Plantaginaceae), caused by species in the genera Podosphaera and Golovinomyces (Erysiphales). There have been recent taxonomic changes in these groups and ongoing disease occurrence on a range of Veronica species previously unrecognized as hosts; however, the pathogen found in Korea remains unidentified. This study aimed to identify the causative agent of powdery mildew on Veronica spp. in Korea through molecular phylogenetic analysis of internal transcribed spacer (ITS) and large subunit (LSU) sequences of rDNA and morphological examination. Our study identified Golovinomyces bolayi as the sole pathogen affecting all nine Veronica species in Korea and confirmed that G. cichoracearum s. str., which was previously identified as the pathogen, was not the causal agent. These results expand the known host range of G. bolayi , including the addition of five Veronica species in Korea: V. hederifolia , V. kiusiana var. glabrifolia , V. longifolia , V. polita , and V. serpyllifolia . Notably, V. kiusiana has been added as a new global host of powdery mildew. These findings substantially enhance our understanding of the taxonomy of powdery mildew pathogens on Veronica species and provide valuable insights into their evolutionary host expansion.

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Section: Discussionmentioning

confidence: 99%

Taxonomy, Phylogeny, and Host Expansion of Powdery Mildew Fungi Parasitic to Veronica Species

Park,

Choi,

Shin

2024

Mycobiology

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First report of powdery mildew caused by Golovinomyces bolayi on okra (Abelmoschus esculentus)

Ardestani

Khodaparast

Moghaddam

et al. 2020

Australasian Plant Dis. Notes

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First Report of Powdery Mildew Caused by Golovinomyces bolayi on Veronica persica in Central China

et al. 2023

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Veronica persica, Persian speedwell, is a flowering plant belonging to the family Plantaginaceae. Due to its showy flowers, this plant is widely planted in many home gardens, city parks and universities in China. From April to June 2021, signs and symptoms of powdery mildew were found on leaves of V. persica growing on the campus of Henan Normal University, Henan Province, China. Signs initially appeared as thin white colonies and subsequently white powdery masses were abundant on the adaxial and abaxial surfaces of leaves and covered up to 99 % of the leaf area. The infected leaves showed chlorotic, deformed or senescence features. About 150 V. persica plants were monitored and more than 90 % of the plants showed these signs and symptoms. Conidiophores (n = 20) were 108 to 220 × 10 to 13 μm and composed of foot cells, followed by short cells and conidia. Conidia were hyaline, doliiform-subcylindrical shaped, 21 to 37 × 15 to 22 μm, and showed distinct fibrosin bodies. Conidial germ tubes were produced at the perihilar position. No chasmothecia were observed. The observed morphological characteristics were consistent with those of previously documented Golovinomyces bolayi (Braun and Cook 2012). To further confirm the powdery mildew fungus, structures of the pathogen were harvested and total genomic DNA was isolated using the method previously described by Zhu et al. (2019, 2021). Using the primers ITS1/ITS4, the internal transcribed spacer (ITS) region of rDNA was amplified (White et al. 1990) and the amplicon was sequenced. The resulting sequence was deposited into GenBank under Accession No. MZ343575 and was 100 % identical (592/592 bp) to G. bolayi on Kalanchoe blossfeldiana (LC417096) (Braun et al. 2019). The additional phylogenetic analysis clearly illustrated that the identified fungus and G. bolayi were clustered in the same branch (Zhu et al. 2022a; Zhu et al. 2022b). To test pathogenicity, healthy V. persica plants were collected from the campus of Henan Normal University and leaf surfaces of three plants were inoculated by dusting fungal conidia from mildew-infested leaves using pressurized air. Three plants without inoculation served as a control. The spore-treated and non-treated plants were separately placed in two growth chambers (temperature, 18℃; humidity, 60%; light/dark, 16h/8h). Seven- to eight-days post-inoculation, pathogen signs were noticeable on inoculated plants, whereas control plants remained healthy. Similar results were obtained by conducting the pathogenicity assays twice. Therefore, based on the analysis, G. bolayi was identified and confirmed as the causal agent of the powdery mildew. This pathogen has been reported on V. persica in Iran (Golmohammadi et al. 2019). However, to our best knowledge, there is no report concerning the powdery mildew caused by G. bolayi on V. persica in China. Recently, G. bolayi was segregated from species clades of G. orontii complex (Braun et al. 2019). Our record of the molecular characterization of G. bolayi will support the further phylogeny and taxonomy analysis of the G. orontii complex. The sudden outbreak of powdery mildew caused by G. bolayi on V. persica may detract from plant health and ornamental value. The identification and confirmation of this disease expands the understanding of this causal agent and will offer support for future powdery mildew control.

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Golovinomyces bolayi causes powdery mildew on Persian speedwell (Veronica persica) in Iran

Cited by 3 publications

References 6 publications

Taxonomy, Phylogeny, and Host Expansion of Powdery Mildew Fungi Parasitic to Veronica Species

Taxonomy, Phylogeny, and Host Expansion of Powdery Mildew Fungi Parasitic to Veronica Species

First report of powdery mildew caused by Golovinomyces bolayi on okra (Abelmoschus esculentus)

First Report of Powdery Mildew Caused by Golovinomyces bolayi on Veronica persica in Central China

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