Gomisin N inhibits adipogenesis and prevents high-fat diet-induced obesity

Jang, Min-Kyung; Yun, Ye‐Rang; Kim, Jihyun; Park, Mi-Hee; Jung, Myeong Ho

doi:10.1038/srep40345

Cited by 43 publications

(37 citation statements)

References 42 publications

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“…In addition, ESEE reduced HFd-induced serum markers of fatty liver, including AST and ALT. These findings were similar to those reported for the anti-obesity effect of Gomisin N in HFd-induced obese mice (26). ESEE reduced HFD-induced increases in serum lipid profiles, such as TC, TG and LdLc, and increased serum levels of HdLc, which were in accordance with its observed suppressive effect on HFd-induced fatty liver and hyperlipidemia in HFd-fed mice.…”

Section: Discussionsupporting

confidence: 90%

Euphorbia supina extract results in inhibition of high‑fat‑diet‑induced obesity in mice

et al. 2018

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The present study was undertaken to investigate the anti‑obesity effect of a 50% ethanol extract of Euphorbia supina (ESEE) in high‑fat‑diet (HFD)‑induced obese C57BL/6J mice. Mice were fed a HFD with or without ESEE (2, 10, or 50 mg/kg) or with Garcinia cambogia (positive control) for 6 weeks. ESEE supplementation significantly reduced body, epididymal white adipose tissue (eWAT), and organ weights (P<0.05). ESEE also reduced hepatic steatosis and improved serum lipid profiles. In addition, ESEE significantly reduced serum leptin levels and increased adiponectin levels, and significantly downregulated the mRNA and protein levels of proliferator‑activated receptor γ (PPARγ) and CCAAT/enhancer‑binding protein alpha (C/EPBα) in eWAT and liver tissues (all P<0.05). These results suggested that ESEE supplementation protects against HFD‑induced obesity by downregulating PPARγ and C/EPBα, and that ESEE may be beneficial for the prevention and treatment of obesity and associated diseases.

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Section: Discussionsupporting

confidence: 90%

Euphorbia supina extract results in inhibition of high‑fat‑diet‑induced obesity in mice

et al. 2018

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“…Another approach would be to limit adipogenesis by inhibiting both the proliferation and differentiation of adipocytes. Plant extracts have been screened to identify compounds that can suppress adipogenesis [8]. One such plant extract that has attracted much interest is Oroxylum indicum.…”

Section: Introductionmentioning

confidence: 99%

Antiadipogenesis of Oroxylum indicum (L.) Kurz Extract via PPARγ2 in 3T3‐L1 Adipocytes

Hengpratom

Ngernsoungnern

et al. 2020

Evidence-Based Complementary and Alternative Medicine

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Oroxylum indicum is regarded as a traditional food with medicinal properties and is used widely throughout Asia. It has previously been demonstrated that O. indicum extract (OIE) was able to suppress the differentiation of 3T3-L1 preadipocytes to adipocytes. However, the mechanism underlying the antiadipogenesis of this plant has not been fully investigated. The present study aimed to explore the impact of OIE at 50 to 200 μg mL−1 on the molecular mechanism involved in the antiadipogenic activity in 3T3-L1 cells at day 0 of their differentiation to adipocytes. The morphology and biochemistry of the cells on day 12 were investigated and compared to the relevant controls. Adiponectin was measured using enzyme-linked immunosorbent assay (ELISA). The mRNA expression of peroxisome proliferator-activated receptor-gamma 2 (PPARγ2), sterol regulatory element-binding proteins 1c (SREBP-1c), fatty acid synthetase (FAS), glucose transporter (GLUT4), and leptin in adipocytes was determined by real-time PCR. The results demonstrated that the OIE at 200 μg mL−1 exhibited strongest suppression on intracellular lipid accumulation. The levels of adiponectin were dramatically increased in the untreated adipocytes, whereas significantly decreased in the 200 μg mL−1 OIE-treated adipocytes (P<0.05). Expression of the mRNAs revealed that OIE-treated adipocytes at 200 μg mL−1 significantly inhibited the expression of PPARγ2 and SREBP-1c and lowered the level of expression of GLUT4, FAS, and leptin compared to the control (P<0.05). These findings suggest that OIE inhibits adipocyte differentiation along with the downregulation of PPARγ2, SREBP-1c, and GLUT4, leading to the decrease in the expression of FAS and adipokine (leptin and adiponectin). Thus, OIE might be developed for hyperlipidemia and obesity prevention.

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“…Among them, 3T3-L1 cells were presented by Howard Green in 1974 and are commonly used as a cell model to study differentiation in adipocytes [7]. Differentiation from preadipocytes to adipocytes consists of three stages: growth arrest, increase by somatic cell replication, and differentiation [8]. The cell cycle of preadipocytes, which have ceased growth by cell conjugation, is activated by cell differentiation-inducing media containing IBMX (3-isobutyl-1-methylxanthine), dexamethasone, and insulin.…”

Section: Introductionmentioning

confidence: 99%

Ergosterol Peroxide from the Medicinal Mushroom Ganoderma lucidum Inhibits Differentiation and Lipid Accumulation of 3T3-L1 Adipocytes

Jeong

Park

2020

IJMS

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Ergosterol peroxide is a natural compound of the steroid family found in many fungi, and it possesses antioxidant, anti-inflammatory, anticancer and antiviral activities. The anti-obesity activity of several edible and medicinal mushrooms has been reported, but the effect of mushroom-derived ergosterol peroxide on obesity has not been studied. Therefore, we analyzed the effect of ergosterol peroxide on the inhibition of triglyceride synthesis at protein and mRNA levels and differentiation of 3T3-L1 adipocytes. Ergosterol peroxide inhibited lipid droplet synthesis of differentiated 3T3-L1 cells, expression of peroxisome proliferator-activated receptor gamma (PPARγ) and CCAT/enhancer-binding protein alpha (C/EBPα), the major transcription factors of differentiation, and also the expression of sterol regulatory element-binding protein-1c (SREBP-1c), which promotes the activity of PPARγ, resulting in inhibition of differentiation. It further inhibited the expression of fatty acid synthase (FAS), fatty acid translocase (FAT), and acetyl-coenzyme A carboxylase (ACC), which are lipogenic factors. In addition, it inhibited the phosphorylation of mitogen-activated protein kinases (MAPKs) involved in cell proliferation and activation of early differentiation transcription factors in the mitotic clonal expansion (MCE) stage. As a result, ergosterol peroxide significantly inhibited the synthesis of triglycerides and differentiation of 3T3-L1 cells, and is, therefore, a possibile prophylactic and therapeutic agent for obesity and related metabolic diseases.

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Gomisin N inhibits adipogenesis and prevents high-fat diet-induced obesity

Cited by 43 publications

References 42 publications

Euphorbia supina extract results in inhibition of high‑fat‑diet‑induced obesity in mice

Euphorbia supina extract results in inhibition of high‑fat‑diet‑induced obesity in mice

Antiadipogenesis of Oroxylum indicum (L.) Kurz Extract via PPARγ2 in 3T3‐L1 Adipocytes

Ergosterol Peroxide from the Medicinal Mushroom Ganoderma lucidum Inhibits Differentiation and Lipid Accumulation of 3T3-L1 Adipocytes

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