Gonadal hormones modulate 5-hydroxytryptamine2A receptors: emphasis on the rat frontal cortex

Cyr, Michel; Bossé, Roger; Paolo, Thérèse Di

doi:10.1016/s0306-4522(97)00445-4

Cited by 96 publications

(63 citation statements)

References 32 publications

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“…Our previous binding studies (Cyr et al 1998) using [ 3 H]ketanserin showed that ovariectomy decreased 5-HT 2A receptor density compared to intact rat values in the dorsal raphe and brain regions of 5-HT projections such as striatum, nucleus accumbens, and frontal cortex, which is corrected by an estradiol treatment. The effect of estradiol to increase 5-HT 2A receptors specific binding of ovariectomized rats was also previously observed using the ligands [ 3 H]-RP62203 (Fink and Sumner 1996), [ 3 H]ketanserin (Sumner and Fink 1995) and [ 3 H]spiperone (Biegon et al 1983).…”

Section: Discussionmentioning

confidence: 97%

“…Autoradiography of 5-HT 2A receptor binding sites with [ 3 H]ketanserin (2 mM, 66.4 Ci/mmol, Amersham) was performed as previously described (Cyr et al 1998). Sections and calibrated standards (Microscales, Amersham) were exposed for 35 days to Amersham Hyperfilm 3 H. The autoradiograms were analyzed using the software package NIH Image 1.63 on a PowerMacintosh 7100 assisted videodensitometry (Sony camera XC-77), which provides values of receptor densities expressed in fmol/mg of tissue.…”

Section: -Ht 2a Receptor Binding Autoradiographymentioning

confidence: 99%

“…The in situ hybridization histochemistry and the posthybridization washes were performed as previously described (Cyr et al 1998). To generate autoradiograms, sections were exposed to Kodak BioMax MR film for two days.…”

Section: -Ht 2a Receptor In Situ Hybridization Histochemistrymentioning

confidence: 99%

“…We have recently shown that sex steroid withdrawal following ovariectomy of rats decreases 5-hydroxytryptamine-2A (5-HT 2A ) receptor density in the dorsal raphe, striatum, nucleus accumbens, and frontal cortex (Cyr et al 1998). Estradiol treatment of ovariectomized rats increases and restores to intact values the 5-HT 2A receptor density in these regions.…”

mentioning

confidence: 99%

“…Estradiol treatment of ovariectomized rats increases and restores to intact values the 5-HT 2A receptor density in these regions. In the frontal cortex, changes in 5-HT 2A receptor mRNA levels are parallel to those of the receptor density after ovariectomy and estradiol treatment (Cyr et al 1998). It is known that interaction of the estrogen receptor (ER)/ligand complex with the promoter estrogen response element regulates estrogen-sensitive gene transcription (Paige et al 1999).…”

mentioning

confidence: 99%

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Modulation by Estrogen-Receptor Directed Drugs of 5-Hydroxytryptamine-2A Receptors in Rat Brain

Cyr

Landry

Paolo

2000

Neuropsychopharmacology

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Section: Discussionmentioning

confidence: 97%

Section: -Ht 2a Receptor Binding Autoradiographymentioning

confidence: 99%

Section: -Ht 2a Receptor In Situ Hybridization Histochemistrymentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Modulation by Estrogen-Receptor Directed Drugs of 5-Hydroxytryptamine-2A Receptors in Rat Brain

Cyr

Landry

Paolo

2000

Neuropsychopharmacology

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Effects of chronic 17?-estradiol treatment on the serotonin 5-HT1A receptor mRNA and binding levels in the rat brain

Österlund

Halldin

Hurd

2000

Synapse

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Acute 17beta-estradiol treatment had been shown to downregulate the 5-HT(1A) receptor mRNA expression in limbic areas of the female rat brain. The aim of the present study was to determine the effects of chronic 17beta-estradiol treatment on 5-HT(1A) receptor mRNA expression and 5-HT(1A) receptor binding in ovariectomized female rats. Using in situ hybridization histochemistry, no alterations were found on the 5-HT(1A) receptor mRNA levels after the estradiol treatment (2 weeks). Radioligand autoradiographic studies using the selective 5-HT(1A) receptor antagonist [(3)H]WAY-100635 revealed reduced receptor binding in the amygdala, hippocampus, perirhinal cortex, and motor cortex after estradiol treatment, whereas no changes were observed in the piriform or retrosplenial cortex. Thus, the previous findings together with the present results indicate that estradiol-induced alterations in 5-HT(1A) receptor mRNA expression appears within hours, but diminishes with chronic treatment when significant changes on the receptor-protein level are apparent. The effects of estradiol treatment on the 5-HT(1A) receptor binding in the limbic areas suggest that estrogen can modulate functions such as learning, memory, cognition, emotional processing, and social behavior. Consequently, estradiol modulation of 5-HT(1A) receptor circuits might be a possible pathway for the estrogen influence in the expression of psychiatric and neurological disorders such as Alzheimer's disease, affective disorders, and schizophrenia.

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Distribution of estrogen receptor α and β in the mouse central nervous system: In vivo autoradiographic and immunocytochemical analyses

Merchenthaler¹,

Lane²,

Numan³

et al. 2004

J of Comparative Neurology

300

259

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Although the distribution of estrogen receptor beta (ERbeta) immunoreactivity in the rat central nervous has been reported, no such data are available in the mouse. The present study used in vivo autoradiography utilizing a (125)I-estrogen that has equal binding affinity for both receptors as well as immunohistochemistry for ERbeta and ERalpha, to investigate and compare the distribution of the two ERs in the mouse CNS. The use specific antisera against ERalpha and ERbeta allowed us to evaluate the contribution of these receptors to the binding detected with autoradiography. In addition, data were collected in ovariectomized wildtype and ERalpha KO (knockout) mice to examine developmental regulation of ERbeta expression by ERalpha. These studies revealed that in the mouse CNS, combining immunoreactivity for ERalpha with that for ERbeta accounted for all regions where binding was seen using autoradiography. Therefore, these data strongly suggest that the major contributors of estrogen binding in the mouse CNS are ERalpha and ERbeta. Together, these data provide an anatomical foundation for future studies and advance our understanding of estrogen action in the CNS. Moreover, since the immunocytochemical images were similar in wildtype and ERalpha KO mice, these studies suggest that the lack of ERalpha does not influence the expression of ERbeta in the central nervous system.

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Gonadal hormones modulate 5-hydroxytryptamine2A receptors: emphasis on the rat frontal cortex

Cited by 96 publications

References 32 publications

Modulation by Estrogen-Receptor Directed Drugs of 5-Hydroxytryptamine-2A Receptors in Rat Brain

Modulation by Estrogen-Receptor Directed Drugs of 5-Hydroxytryptamine-2A Receptors in Rat Brain

Effects of chronic 17?-estradiol treatment on the serotonin 5-HT1A receptor mRNA and binding levels in the rat brain

Distribution of estrogen receptor α and β in the mouse central nervous system: In vivo autoradiographic and immunocytochemical analyses

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