Gonadotropin-Releasing Hormone and Gonadal Steroids Regulate Transcription Factor mRNA Expression in Primary Pituitary and Immortalized Gonadotrope Cells

Zheng, Weiming; Grafer, Constance M.; Kim, Jonathan; Halvorson, Lisa M.

doi:10.1177/1933719114565031

Cited by 5 publications

(2 citation statements)

References 86 publications

(126 reference statements)

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“…For example, transcripts of sf1 decreased along with several other steroidogenic genes in developing female trout exposed to androgen 11β-hydroxyandrostenedione (10 mg per kg of food; Oncorhynchus mykiss; Baron et al, 2008). Furthermore, Zheng et al (2015) observed that 5α-DHT significantly decreased sf1 expression in rat primary pituitary cell cultures. The 5α-DHT-mediated decrease in sf1 expression may have influenced subsequent dax-1 and sox9 transcription in NF stage 46 S. tropicalis as studies have reported that Sf1 initiates transcription of dax-1 (Hoyle et al, 2002) and sox9 (Sekido et al, 2008(Sekido et al, , 2004.…”

Section: Discussionmentioning

confidence: 94%

Expression of sf1 and dax-1 are regulated by thyroid hormones and androgens during Silurana tropicalis early development

Campbell

Langlois

2018

General and Comparative Endocrinology

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Thyroid hormones (THs) and androgens have been shown to be extensively involved in sexual development; however, relatively little is known with regard to TH-related and androgenic actions in sex determination. We first established expression profiles of three sex-determining genes (sf1, dax-1, and sox9) during the embryonic development of Western clawed frogs (Silurana tropicalis). Transcripts of sf1 and sox9 were detected in embryos before the period in which embryonic transcription commences indicating maternal transfer, whereas dax-1 transcripts were not detected until later in development. To examine whether TH status affects sex-determining gene expression in embryonic S. tropicalis, embryos were exposed to co-treatments of iopanoic acid (IOP), thyroxine (T4), or triiodothyronine (T3) for 96 h. Expression profiles of TH receptors and deiodinases reflect inhibition of peripheral deiodinase activity by IOP and recovery by T3. Relevantly, elevated TH levels significantly increased the expression of sf1 and dax-1 in embryonic S. tropicalis. Further supporting TH-mediated regulation, examination of the presence and frequency of transcription factor binding sites in the putative promoter regions of sex-determining genes in S. tropicalis and rodent and fish models using in silico analysis also identified TH motifs in the putative promoter regions of sf1 and dax-1. Together these findings advocate that TH actions as early as the period of embryogenesis may affect gonadal fate in frogs. Mechanisms of TH and androgenic crosstalk in relation to the regulation of steroid-related gene expression were also investigated.

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Section: Discussionmentioning

confidence: 94%

Expression of sf1 and dax-1 are regulated by thyroid hormones and androgens during Silurana tropicalis early development

Campbell

Langlois

2018

General and Comparative Endocrinology

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“…The bioinformatics analysis described in this study showed that the 13-bp indel sequence might alter GATA2 TF binding. GATA2 is essential for the differentiation of endometrial stromal cells (ESCs) (Kin et al, 2015), and both GATA2 and GATA4 are involved in transcriptional regulation of gonadotropin genes (Zheng et al, 2015). In summary, IGF2BP2 may bind GATA2 through the P4-Ins-13bp insertion sequence, reducing the mRNA expression of IGF2BP2 in the multi-lamb group.…”

Section: Discussionmentioning

confidence: 99%

Insertion/deletion variants within the IGF2BP2 gene identified in reported genome-wide selective sweep analysis reveal a correlation with goat litter size

Xin

Bai

et al. 2021

J. Zhejiang Univ. Sci. B

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Insulin-like growth factor 2 mRNA-binding protein 2 (IGF2BP2, also called IMP2) plays an essential role in the development and maturation of germ cells and embryos and is a candidate gene for goat litter size, based on a previous genomewide selective sweep analysis. In this study, the mRNA expression level of IGF2BP2 was found to be significantly higher in a single-lamb group than in a multi-lamb group. Insertions/deletions (indels) within the goat IGF2BP2 gene, including P4-Ins-13bp and P5-Del-12bp, were verified in 918 Shaanbei White Cashmere (SBWC) female goats. The minor allelic frequencies (MAFs) of P4-Ins-13bp and P5-Del-12bp loci were 0.349 and 0.295, respectively. Analysis using the Chi-square (χ 2 ) test showed that the genotype (χ 2 =14.479, P=0.006) distribution of P4-Ins-13bp was significantly different between the single-lamb and multi-lamb groups. Correlation analysis demonstrated that P4-Ins-13bp was significantly associated with goat litter size (P= 0.022), and individual goats with the homozygous deletion/deletion (DD) genotype produced more litters than other goats. Therefore, considered as a potential molecular marker significantly related to lambing traits, the P4-Ins-13bp mutation of the goat IGF2BP2 gene can be used in goat breeding with practical molecular marker-assisted selection (MAS) to optimize female reproduction and improve economic efficiency in the goat industry.

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A novel inducible lentiviral system for multi-gene expression with human HSP70 promoter and tetracycline-induced promoter

et al. 2017

Appl Microbiol Biotechnol

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Despite lentiviral system's predominance, its ultimate potential for gene therapy has not been fully exploited. Currently, most lentivirus vectors are non-inducible expression system or single-gene-induced system, which limits the extensive application in gene therapy. In this study, we designed a novel lentiviral vector containing HSP70 promoter and TRE promoter. Compared to traditional lentiviral vectors and inducible vectors, our controllable system has many advantages. Firstly, it contains multiple gene or shRNA targets. Secondly, genes expression is on/off in response to heat shock and DOX induction in time of need respectively with high effectivity and sensitivity. Thirdly, TRE promoter and HSP70 promoter can work with no interference from each other in the same inducible lentiviral vector. In addition, our study also shows that our novel vector has a higher downstream gene expression efficiency than co-transfection method and can co-position multi-genes in single cell effectively. Finally, we propose four derived models based on our vector at the end, which may be useful in biological research and clinical research in the future. Therefore, we believe that this novel lentiviral system could be promising in gene therapy for tumor.

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Gonadotropin-Releasing Hormone and Gonadal Steroids Regulate Transcription Factor mRNA Expression in Primary Pituitary and Immortalized Gonadotrope Cells

Cited by 5 publications

References 86 publications

Expression of sf1 and dax-1 are regulated by thyroid hormones and androgens during Silurana tropicalis early development

Expression of sf1 and dax-1 are regulated by thyroid hormones and androgens during Silurana tropicalis early development

Insertion/deletion variants within the IGF2BP2 gene identified in reported genome-wide selective sweep analysis reveal a correlation with goat litter size

A novel inducible lentiviral system for multi-gene expression with human HSP70 promoter and tetracycline-induced promoter

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