Gonadotropins induce accumulation of insulin-like growth factor I mRNA in pig granulosa cells in vitro

Hatey, François; Langlois, Isabelle; Mulsant, Philippe; Bonnet, Agnès; Benne, Francis; Gasser, F.

doi:10.1016/0303-7207(92)90145-v

Cited by 35 publications

(17 citation statements)

References 28 publications

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“…These results directly show that the ovarian follicle of red seabream can produce IGF-I. Moreover, similar to the mammals (Hatey et al 1992), the stimulation of IGF-I gene transcription is required for the production of IGF-I in the ovarian follicles of red seabream. Although endocrine factors involved in IGF-I production in the ovarian follicle of red seabream have not yet been identified, FSH, LH, E 2 , GH, and other growth factors are thought to be candidates for IGF-I production, as suggested in mammalian species (Hsu and Hammond 1987;Mondschein and Hammond 1988).…”

Section: -5 Sites Of Igf-i Production and Its Physiologi-mentioning

confidence: 54%

Oogenesis in Teleost Fish

Kagawa¹

2013

Aqua-BioSci. Monogr. (ABSM)

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Section: -5 Sites Of Igf-i Production and Its Physiologi-mentioning

confidence: 54%

Oogenesis in Teleost Fish

Kagawa¹

2013

Aqua-BioSci. Monogr. (ABSM)

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“…Up-regulation of IGF-1 by gonadotropins has also been reported in rat preovulatory follicles 25 and pig granulosa cells. 26 IGF-1-mediated inhibition of apoptosis without effect on the expression of bcl-2, bcl-x or bax has been reported in COS cells. 27 IGF-1 has been shown to activate serine-threonine kinase Akt, which subsequently inactivates anti-apoptotic protein Bad and inhibits apoptosis.…”

Section: Discussionmentioning

confidence: 99%

Human ovarian surface epithelial (OSE) cells express LH/hCG receptors, and HCG inhibits apoptosis of OSE cells via up‐regulation of insulin‐like growth factor‐1

et al. 2001

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“…Several in vitro and in vitro studies have established that the pig ovary is a site of production and action of insulinlike growth factors (IGFs) as well as their binding proteins (IGFBPs) [1][2][3][4][5][6]. IGFs play an important role in follicular growth by stimulating granulosa cell proliferation and synergizing with FSH in granulosa cell differentiation [7,8].…”

Section: Introductionmentioning

confidence: 99%

Proteolytic Activity Degrading Insulin-like Growth Factor-Binding Protein−2, −3, −4, and −5 in Healthy Growing and Atretic Follicles in the Pig Ovary1

Besnard

Pisselet

Monniaux

et al. 1997

Biology of Reproduction

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In the pig, ovarian follicular growth is characterized by an increase in intrafollicular levels of insulin-like growth factor-binding protein (IGFBP)-3 and a decrease in the levels of IGFBPs < 40 kDa (IGFBP-2, -4 and, to a lesser extent, a 30-kDa IGFBP likely corresponding to IGFBP-5). In contrast, atresia is primarily associated with a strong increase in intrafollicular levels of IGFBP-2 and -4, with intrafollicular levels of IGFBP-3 and -5 varying slightly or not at all. The purpose of the present study was to determine whether intrafollicular proteases are involved in such changes. Porcine follicular development was synchronized with a progestin, and individual follicles were isolated 12 h and 96 h after progestin withdrawal. Follicular fluid from follicles of various sizes and qualities was collected and incubated alone or with a source of exogenous bovine IGFBP-2 or human IGFBP-3, -4, or -5 for 20 h at 37 degrees C. Samples were then analyzed by Western ligand blotting and by immunoblotting using specific antisera. Porcine follicular fluid from various classes of follicles contained proteolytic activity degrading IGFBP-2, -4, and -5. In contrast, intrafollicular IGFBP-3 proteolytic activity was very low or nondetectable. In preovulatory follicles, degradation of IGFBPs < 40 kDa was 1) accompanied by the generation of small proteolytic fragments visualized by immunoblotting, 2) strongly inhibited by EDTA and 1,10-phenanthroline, and 3) dependent on the presence of zinc and calcium chloride. PMSF (1 mM, serine protease inhibitor) inhibited degradation of IGFBP-2 and to a lesser extent IGFBP-4, but not IGFBP-5. Other serine and cysteine protease inhibitors as well as TIMP-2 and BB-2116 (natural tissue inhibitor-2 and synthetic inhibitor of matrix metalloproteinases [MMPs], respectively) were ineffective. Gelatin-substrate zymography revealed the presence of two major intrafollicular gelatinase MMPs at 60 kDa and 76-85 kDa (likely MMPs 2 and 9, respectively), the levels of which decreased (76-85 kDa) or strongly increased (60 kDa) during follicular atresia. Follicular growth at diameters between 2 and 6-7 mm was characterized by a dramatic increase in proteolytic activity degrading IGFBP-2, -5 and, to a lesser extent, IGFBP-4. Atresia, in contrast, was associated with a marked decrease in proteolytic activity degrading IGFBP-2, -4, and -5. These results suggest that 1) changes in proteolytic activity of intrafollicular IGFBPs < 40 kDa are at least partly responsible for the changes in intrafollicular IGFBP levels during follicular growth and atresia in the pig and 2) calcium- and zinc-dependent metalloprotease(s) as well as serine protease(s) are involved in degradation of intrafollicular IGFBPs < 40 kDa.

show abstract

Gonadotropins induce accumulation of insulin-like growth factor I mRNA in pig granulosa cells in vitro

Cited by 35 publications

References 28 publications

Oogenesis in Teleost Fish

Oogenesis in Teleost Fish

Human ovarian surface epithelial (OSE) cells express LH/hCG receptors, and HCG inhibits apoptosis of OSE cells via up‐regulation of insulin‐like growth factor‐1

Proteolytic Activity Degrading Insulin-like Growth Factor-Binding Protein−2, −3, −4, and −5 in Healthy Growing and Atretic Follicles in the Pig Ovary1

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