Gonococcal bacterial load in PrEP users with Mycoplasma genitalium coinfection

Dijck, Christophe Van; Baetselier, Irith De; Cuylaerts, Vicky; Buyze, Jozefien; Laumen, Jolein; Vuylsteke, Béa; Kenyon, Chris

doi:10.1177/09564624211048678

Cited by 3 publications

(3 citation statements)

References 27 publications

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“…This is, to the authors' knowledge, the largest study to assess the urogenital MG and CT load simultaneously. Contrary to other studies in NG and HIV, we did not observe a higher MG load with a CT coinfection 6,7 . The high number of patients with a coinfection is, thus, likely because of patient behavioral characteristics, more than bacterial synergistic effects.…”

Section: Discussioncontrasting

confidence: 99%

“…4 Chlamydia trachomatis has been implicated in the same clinical syndromes and sequelae as MG. 1,2 Both CT and MG are suggested to increase the transmissibility and acquisition of HIV. 5,6 Also, the small study by Van Dijck et al 7 suggested that the presence of an MG and Neisseria gonorrhoeae (NG) coinfection increased the bacterial load at the anatomical location, possibly impacting gonococcal transmissibility. A higher bacterial load might indicate a higher transmission potential.…”

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confidence: 99%

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Chlamydia trachomatis Coinfection Does Not Influence Mycoplasma genitalium Bacterial Load in Urogenital Samples

et al. 2022

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Background: Mycoplasma genitalium (MG) is associated with urethritis in men and weakly associated with pelvic inflammatory disease in women. Mycoplasma genitalium coinfections with Neisseria gonorrhoeae (NG) and Chlamydia trachomatis (CT) are commonly reported; however, little is known about their interaction. One study suggested that MG/NG coinfections might increase the bacterial load of NG, which has been shown to have a higher transmission potential. As even less is known about the impact of a simultaneous MG/CT infection, we assessed whether patients with urogenital MG/CT coinfections have a higher bacterial load than patients with a single infection.Methods: There were 1673 urogenital samples from patients from a population-based chlamydia study, and our sexually transmitted infection clinic tested for both CT and MG. When positive, the load was quantified. Nonparametric tests compared the CT and MG load, and linear regression analyses tested the association of the CT and MG load within a patient. Results:In 60 MG-positive patients, MG load ranged from 1.7 to 6.0 log10 copies/ml, similar to the CT load distribution. Only 6 patients were MG-positive and CT-negative, but the MG load distribution was similar to that of CT-positive patients (n.s.). The MG and CT load was unrelated in coinfected persons (n.s.). Conclusions:We found no correlation between the CT and MG load in urogenital samples, and the MG load distribution was similar in CT-positive and CT-negative patients. These results could have implications for the transmission risk of these infections.

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confidence: 99%

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confidence: 99%

Chlamydia trachomatis Coinfection Does Not Influence Mycoplasma genitalium Bacterial Load in Urogenital Samples

et al. 2022

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“…In a recent HIV pre-exposure prophylaxis (PrEP) demonstration trial involving 200 men, molecular testing for N. gonorrhoeae, C. trachomatis, and M. genitalium found significantly higher gonococcal bacterial loads in the presence of M. genitalium, suggesting that M. genitalium may be a cofactor for N. gonorrhoeae transmission. If this finding is confirmed in other studies, M. genitalium may be required as a cofactor for N. gonorrhoeae transmission in specific populations such as MSM (Van Dijck et al, 2022). Therefore, future studies should investigate co-infections with M. genitalium and other sexually transmitted bacteria (e.g., C. trachomatis or N. gonorrhoeae; Desdorf et al, 2021).…”

Section: Co-infection With Mycoplasma Genitaliummentioning

confidence: 82%

Mycoplasma genitalium infection in the female reproductive system: Diseases and treatment

Zhou

Luo

et al. 2023

Front. Microbiol.

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Mycoplasma genitalium is a newly emerged sexually transmitted disease pathogen and an independent risk factor for female cervicitis and pelvic inflammatory disease. The clinical symptoms caused by M. genitalium infection are mild and easily ignored. If left untreated, M. genitalium can grow along the reproductive tract and cause salpingitis, leading to infertility and ectopic pregnancy. Additionally, M. genitalium infection in late pregnancy can increase the incidence of preterm birth. M. genitalium infections are often accompanied by co-infection with other sexually transmitted pathogens (Chlamydia trachomatis, Neisseria gonorrhoeae, and Trichomonas vaginalis) and viral infections (Human Papilloma Virus and Human Immunodeficiency Virus). A recent study suggested that M. genitalium plays a role in tumor development in the female reproductive system. However, few studies endorsed this finding. In recent years, M. genitalium has evolved into a new “superbug” due to the emergence of macrolide-and fluoroquinolone-resistant strains leading to frequent therapy failures. This review summarizes the pathogenic characteristics of M. genitalium and the female reproductive diseases caused by M. genitalium (cervicitis, pelvic inflammatory disease, ectopic pregnancy, infertility, premature birth, co-infection, reproductive tumors, etc.), as well as its potential relationship with reproductive tumors and clinical treatment.

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Integrated microscale immiscible phase extraction and isothermal amplification for colorimetric detection of Neisseria gonorrhoeae

Rodriguez-Mateos,

Ngamsom,

Ameyo

et al. 2023

Anal Bioanal Chem

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Gonorrhea is the second most common sexually transmitted infection (STI) with around 87 million cases worldwide estimated in 2016 by the World Health Organization. With over half of the cases being asymptomatic, potential life-threatening complications and increasing numbers of drug-resistant strains, routine monitoring of prevalence and incidence of infections are key preventive measures. Whilst gold standard qPCR tests have excellent accuracy, they are neither affordable nor accessible in low-resource settings. In this study, we developed a lab-on-a-chip platform based on microscale immiscible filtration to extract, concentrate and purify Neisseria gonorrhoeae DNA with an integrated detection assay based on colorimetric isothermal amplification. The platform was capable of detecting as low as 500 copies/mL from spiked synthetic urine and showed no cross-reactivity when challenged with DNAs from other common STIs. The credit card–size device allows DNA extraction and purification without power or centrifuges, and the detection reaction only needs a low-tech block heater, providing a straightforward and visual positive/negative result within 1 h. These advantages offer great potential for accurate, affordable and accessible monitoring of gonorrhea infection in resource-poor settings.

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Gonococcal bacterial load in PrEP users with Mycoplasma genitalium coinfection

Cited by 3 publications

References 27 publications

Chlamydia trachomatis Coinfection Does Not Influence Mycoplasma genitalium Bacterial Load in Urogenital Samples

Chlamydia trachomatis Coinfection Does Not Influence Mycoplasma genitalium Bacterial Load in Urogenital Samples

Mycoplasma genitalium infection in the female reproductive system: Diseases and treatment

Integrated microscale immiscible phase extraction and isothermal amplification for colorimetric detection of Neisseria gonorrhoeae

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