Good agreement of conventional and gel-based direct agglutination test in immune-mediated haemolytic anaemia

Piek, Christine J.; Teske, Erik; Leeuwen, M.W. van; Day, Michael

doi:10.1186/1751-0147-54-10

Cited by 12 publications

(17 citation statements)

References 21 publications

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“…A number of other papers yielded a negligible level of evidence for neoplasia as a cause of IMHA. These documented the presence of carcinomas, malignant histiocytosis, and other hematopoietic tumors, myeloid neoplasia, multiple myeloma, sarcomas, and miscellaneous undefined tumors in dogs with IMHA.…”

Section: Cancermentioning

confidence: 96%

“…Overall, 66 manuscripts were reviewed . The IME values were calculated for 27 infectious agents or types of infection (Figures ), but could not be calculated for many infectious agents either because of the way data were summarized (eg, the number of individual patients with IMHA and an infection could not be discerned) or because an individual patient with IMHA had >1 comorbidity (Supporting Information S2).…”

Section: Putative Trigger Factorsmentioning

confidence: 99%

“…Seventeen studies documented 103 cases of IMHA in Babesia ‐infected dogs . The IME values for Babesia species as a whole ranged from 0.00 to 6.99, with a median of 4.55.…”

Section: Putative Trigger Factorsmentioning

confidence: 99%

“…One study documented IMHA in 2 chronically infected splenectomized mixed breed dogs experimentally infected with what was thought to be B. gibsoni , but later characterized as Babesia conradae , yielding an IME value of 6.25. Five studies documented 13 cases of IMHA in dogs infected with Babesia canis , with a median IME value of 3.20 and range of 0‐4.32 . Babesia vogeli was documented in 2 studies of 5 dogs with IMHA, with IME values of 5.73 and 4.14.…”

Section: Putative Trigger Factorsmentioning

confidence: 99%

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ACVIM consensus statement on the diagnosis of immune‐mediated hemolytic anemia in dogs and cats

Garden

Kidd

Mexas

et al. 2019

Veterinary Internal Medicne

132

196

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Immune‐mediated hemolytic anemia (IMHA) is an important cause of morbidity and mortality in dogs. IMHA also occurs in cats, although less commonly. IMHA is considered secondary when it can be attributed to an underlying disease, and as primary (idiopathic) if no cause is found. Eliminating diseases that cause IMHA may attenuate or stop immune‐mediated erythrocyte destruction, and adverse consequences of long‐term immunosuppressive treatment can be avoided. Infections, cancer, drugs, vaccines, and inflammatory processes may be underlying causes of IMHA. Evidence for these comorbidities has not been systematically evaluated, rendering evidence‐based decisions difficult. We identified and extracted data from studies published in the veterinary literature and developed a novel tool for evaluation of evidence quality, using it to assess study design, diagnostic criteria for IMHA, comorbidities, and causality. Succinct evidence summary statements were written, along with screening recommendations. Statements were refined by conducting 3 iterations of Delphi review with panel and task force members. Commentary was solicited from several professional bodies to maximize clinical applicability before the recommendations were submitted. The resulting document is intended to provide clinical guidelines for diagnosis of, and underlying disease screening for, IMHA in dogs and cats. These should be implemented with consideration of animal, owner, and geographical factors.

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Section: Cancermentioning

confidence: 96%

Section: Putative Trigger Factorsmentioning

confidence: 99%

“…Seventeen studies documented 103 cases of IMHA in Babesia ‐infected dogs . The IME values for Babesia species as a whole ranged from 0.00 to 6.99, with a median of 4.55.…”

Section: Putative Trigger Factorsmentioning

confidence: 99%

Section: Putative Trigger Factorsmentioning

confidence: 99%

See 2 more Smart Citations

ACVIM consensus statement on the diagnosis of immune‐mediated hemolytic anemia in dogs and cats

Garden

Kidd

Mexas

et al. 2019

Veterinary Internal Medicne

132

196

View full text Add to dashboard Cite

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“…This improper test antibody to anti‐RBC Ig ratio leads to false‐negative results if there are not enough serial serum dilutions tested. A prozone effect was observed in 17 of 126 (13%) samples tested by Piek et al…”

Section: Diagnosis Of Imhamentioning

confidence: 92%

The utility of diagnostic tests for immune‐mediated hemolytic anemia

MacNeill

Dandrieux

Lubas

et al. 2019

Veterinary Clinical Pathol

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Background:A definitive diagnosis of immune-mediated hemolytic anemia (IMHA) can be difficult to make. However, it is critical to differentiate IMHA from other causes of anemia due to the impact on prognosis and outcome for IMHA patients. Recently published American College of Veterinary Internal Medicine recommendations for the diagnosis of IMHA should be followed to concurrently confirm ongoing anemia, verify in vivo hemolysis, and detect anti-erythrocyte antibodies. The reliability of immunologic IMHA tests varies depending on which test is used and how it is performed.Objectives: Our aims were to determine which tests are currently used in veterinary medicine to diagnose IMHA and review the utility of assays that have historically been used to diagnose IMHA. Methods:A short survey was designed to see which diagnostic tests for IMHA were currently being used by veterinary practices. The survey was distributed via listserves to veterinarians and veterinary technologists. A literature review was performed to report the utility of diagnostic tests for the diagnosis of IMHA.Results: Survey respondents indicated a variability in test protocols used to diagnose IMHA. Most respondents perform saline agglutination or Coombs' tests to detect anti-erythrocyte antibodies. Additional tests that can be used to support a diagnosis of IMHA are discussed in this review. Conclusions:A standardized diagnostic approach should be followed to differentiate IMHA from other causes of anemia. Test methodology can vary from one laboratory to another, and clinicians should be familiar with the procedures used by their laboratory.

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