In modern society, humans spend most of their time in the indoor environment (home, work, school...). This indoor lifestyle constantly poses challenges to our physical and mental health by affecting our hormone levels and circadian rhythm. This article deals with the possible connection between human and working environment factors and the emergence of health problems in connection with work. Using statistical methods, the dependence between the six characteristics (age, length of employment, visual demand of the work task, satisfaction with lighting conditions, and shift work) and occurrence of health-related problems and discomfort (i.e., eye discomfort, headache, eye fatigue, and seasonal affective disorder symptoms) was examined. The paper also deals with the evaluation of lighting conditions in the workshop using the lighting design software DIALux evo 9.2. Using this software, two lighting variants were modeled. The first simulated variant included lighting parameters according to the currently used luminaries and the second variant contained more efficient LED luminaries.