Gossypin inhibits gastric cancer growth by direct targeting of <scp>AURKA</scp> and <scp>RSK2</scp>

Wang, Li; Wang, Xiangyu; Chen, Hanyong; Zu, Xueyin; Ma, Fayang; Liu, Kangdong; Bode, Ann M.; Dong, Zigang; Kim, Dong Joon

doi:10.1002/ptr.6253

Cited by 25 publications

(23 citation statements)

References 49 publications

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“…AURKA is overexpressed in a variety of human cancers, including gastric cancer, breast cancer, bladder cancer, head and neck squamous cell carcinoma and HCC [39][40][41]. And it plays an important role in the mitotic cell cycle by participating in centrosome replication, isolation and maturation [42][43][44].…”

Section: Discussionmentioning

confidence: 99%

WITHDRAWN: A Bioinformatics-Based Screening and Analysis of Key Genes in Hepatocellular Carcinoma

Wei

Wang

Chen

et al. 2020

Preprint

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Hepatocellular carcinoma (HCC) is considered as the leading killer disease in the world. So far most of the diagnosis of HCC is mainly established on imaging and biopsy. As sequencing technology is developing quite fast, and it has already been widely applied in the medical area, such as cancer diagnosis. In this article, GSE121248, GSE76427 and GSE60502 datasets were chosen to analyze and screen key genes which could affect the development of liver cancer through the bioinformatics method. The results showed up regulated genes mainly reside in cell division, nucleus, protein binding pathway, and down regulated genes are mostly located in the Oxidation-reduction process, Extracellular region, Heme binding, Metabolic pathway. Secondly, hub gene analysis indicated there were twelve critical hub genes found: RFC4, RACGAP1, CCNB2, CDC20, UBE2C, PTTG1, AURKA, PRC1, NCAPG, CDKN3, TOP2A, KIF20A, AURKA and CDKN3. By applying bioinformatic measures , the genes associated with hepatocellular carcinoma can be efficiently analyzed, that would provide invaluable information for translational studies.

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Section: Discussionmentioning

confidence: 99%

WITHDRAWN: A Bioinformatics-Based Screening and Analysis of Key Genes in Hepatocellular Carcinoma

Wei

Wang

Chen

et al. 2020

Preprint

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“…Flavonoid could protect human sperm against the damaging effects of FeSO 4 /H 2 O 2 (Huang et al 2014). Recently, it has been found that flavone works against cancer growth by direct targeting (Ganai 2017;Sajjadi et al 2018;Wang et al 2018b), and inhibits migration through DLC1/RhoA pathway by decreasing ROS generation in cancer cells (Zhu et al 2016).…”

Section: Introductionmentioning

confidence: 99%

Improved Preimplantation Development of Porcine Cloned Embryos by Flavone Supplement as Antioxidant

Fang

Qamar

Yoon

et al. 2018

J Anim Reprod Biotechnol

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This experiment was conducted to analyse the effects of flavone supplementation on the preimplantation development of in-vitro produced porcine embryos. During in-vitro development, immature oocytes and early embryos were exposed to different concentrations of flavone (0, 1μM, 25μM, 50 μM, and 100 μM respectively). Results showed that 100 µM of flavone significantly reduced the intracellular ROS levels of oocytes accompanied with a significant rise in GSH level. In parthenogenesis, no significant change was observed in the cleavage rates whether flavone was supplemented in IVM or IVC media. In IVM supplemented group, the blastocyst development rate was significantly enhanced by 1 µM concentration than other groups (51.5% vs. 41.3%, 44.0%, 36.3%, 31.7%; P<0.05) respectively. However, in IVC group 1 µM concentration significantly improved the blastocysts production than 50 µM and control groups (50.0% vs. 40.5%, 38.0%; P<0.05) respectively. Following nuclear transfer, the cleavage rate of IVM group was significantly more in 1 µM than 50 µM and 100 µM groups (92.9% vs. 89.7%, 87.8%; P<0.05), followed by similar pattern of cloned blastocysts production being significantly higher in 1 µM group than 50 µM, 100 µM and control groups (16.8% vs. 9.0%, 7.1%, 12.8%; P<0.05) respectively. In IVC group, 1 µM concentration resulted in significantly higher cleavage rate than 25 µM and 50 µM groups (91.7% vs. 87.8%, 88.8%; P<0.05) respectively. However, the blastocysts production was significantly higher in 100 µM group than others (26.2% vs. 13.6%, 14.0%, 18.2%; P<0.05) respectively. The optimal concentrations of flavone significantly enhanced the percentages of ICM:TE than control group (43.8% vs. 37.6%; P<0.05) accompanied with significantly higher expression levels of reprogramming related genes. In conclusion, the optimal concentrations of 1 µM during IVM and 100 µM during IVC can significantly improve the production of porcine in-vitro embryos.

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“…Lapachol is a 1,4‐naphthoquinone compound (Figure a). Previously, JB6 mouse epithelial cells have been used to identify potential molecular targets of selected compounds, and MAPK and AKT signaling are strongly activated by epidermal growth factor (EGF) stimulation (Wang et al, ). Therefore, to identify potential molecular targets of lapachol, we first investigated whether lapachol could affect EGF‐induced various signaling molecules in JB6 mouse epithelial cells.…”

Section: Resultsmentioning

confidence: 99%

“…Therefore, we suggest that lapachol can inhibit the activity of RSK2 and lead to reduced expression of cell cycle marker proteins. Additionally, inhibition of phosphorylated RSK could induce apoptosis by upregulating the expression levels of cleaved caspases, PARP, and cytochrome c (Wang et al, ). Therefore, we next investigated whether lapachol could induce apoptosis of ESCC cells.…”

Section: Discussionmentioning

confidence: 99%

Lapachol is a novel ribosomal protein S6 kinase 2 inhibitor that suppresses growth and induces intrinsic apoptosis in esophageal squamous cell carcinoma cells

Xie

Zhang

et al. 2019

Phytotherapy Research

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Lapachol is a 1,4‐naphthoquinone that is isolated from the Bignoniaceae family. It has been reported to exert anti‐inflammatory, antibacterial, and anticancer activities. However, the anticancer activity of lapachol and its molecular mechanisms against esophageal squamous cell carcinoma (ESCC) cells have not been fully investigated. Herein, we report that lapachol is a novel ribosomal protein S6 kinase 2 (RSK2) inhibitor that suppresses growth and induces intrinsic apoptosis in ESCC cells. We found that lapachol strongly attenuates downstream signaling molecules of RSK2 in ESCC cells and also directly inhibits RSK2 activity in vitro. The RSK protein is highly activated in ESCC cells and knockdown of RSK2 significantly suppresses anchorage‐dependent and anchorage‐independent growth of ESCC cells. Additionally, lapachol inhibits anchorage‐dependent and anchorage‐independent growth of ESCC cells, and the inhibition of cell growth by lapachol is dependent on the expression of RSK2. We also found that lapachol induces mitochondria‐mediated cellular apoptosis by activating caspases‐3, ‐7, and PARP, inducing the expression of cytochrome c and BAX by inhibiting downstream molecules of RSK2. Overall, lapachol is a potent RSK2 inhibitor that might be used for chemotherapy against ESCC.

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Gossypin inhibits gastric cancer growth by direct targeting of AURKA and RSK2

Cited by 25 publications

References 49 publications

WITHDRAWN: A Bioinformatics-Based Screening and Analysis of Key Genes in Hepatocellular Carcinoma

WITHDRAWN: A Bioinformatics-Based Screening and Analysis of Key Genes in Hepatocellular Carcinoma

Improved Preimplantation Development of Porcine Cloned Embryos by Flavone Supplement as Antioxidant

Lapachol is a novel ribosomal protein S6 kinase 2 inhibitor that suppresses growth and induces intrinsic apoptosis in esophageal squamous cell carcinoma cells

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