Gossypol and related compounds are produced and accumulate in the aboveground parts of the cotton plant, independent of roots as the source

Pandeya, Devendra; Campbell, LeAnne M.; Puckhaber, Lorraine S.; Suh, Charles P.-C.; Rathore, Keerti S.

doi:10.1007/s00425-022-04049-0

Cited by 3 publications

(7 citation statements)

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“…The biosynthetic steps of gossypol have been extensively studied (Wagner et al, 2012;Tian et al, 2018;Lin et al, 2023); however, the synthetic site of gossypol remains controversial. Researchers have reported that the gland-contained terpenoids are either 'produced in the roots and transported and stored in New Phytologist the pigment gland of the aboveground tissues' or 'produced in a localized manner and retained therein' (Cai et al, 2010;Pandeya et al, 2022).…”

Section: Discussionmentioning

confidence: 99%

“…The recent efforts to study the relationship between the pigment gland and gland-contained terpenoids have progressed much, yielding an interesting novel theory that the root is not the source of gossypol and the related terpenoids in the aerial tissues of cotton. Pandeya et al (2022) performed grafted transgenic cotton with ultra-low gossypol cottonseed as the scion on a WT rootstock. The study found that gossypol produced in WT roots failed to replenish the gossypol content of the ultra-low gossypol cottonseed, implying that the gossypol accumulation in the aboveground parts is unrelated to the roots.…”

Section: Discussionmentioning

confidence: 99%

“…Pandeya et al . (2022) performed grafted transgenic cotton with ultra‐low gossypol cottonseed as the scion on a WT rootstock. The study found that gossypol produced in WT roots failed to replenish the gossypol content of the ultra‐low gossypol cottonseed, implying that the gossypol accumulation in the aboveground parts is unrelated to the roots.…”

Section: Discussionmentioning

confidence: 99%

“…This observation suggests that the gossypol biosynthetic pathway is highly enriched in gland cells, thereby increasing the metabolic flow for gossypol and related terpenoid production. These findings and previous publications confirmed that gossypol and related terpenoids stored in the gland cavity are produced in the localized pigment gland cells (Pandeya et al ., 2022; Long et al ., 2023; Sun et al ., 2023). Studies on other plant species also supported the notion that glandular trichomes are typically the synthetic sites of the secondary metabolites they store or secrete (Glas et al ., 2012; Xu et al ., 2018).…”

Section: Discussionmentioning

confidence: 99%

“…Cotton roots produced a different set of terpenoids from other tissues. The green tissues produced gossypol, hemigossypolone and heliocides, while the roots produced gossypol, hemigossypol, desoxyhemigossypol, gossypol‐6‐methyl ether, gossypol‐6,6‐dimethyl ether, hemigossypol‐6‐methyl ether and desoxyhemigossypol‐6,6‐methyl ether (Veech & McClure, 1977; Sunilkumar et al ., 2006; Pandeya et al ., 2022). These different compositions of terpenoids reflects the metabolic flux variation in terpenoids biosynthesis, implying different regulation mechanism of the gossypol biosynthetic pathway among the different cotton tissues.…”

Section: Discussionmentioning

confidence: 99%

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The dual role of GoPGF reveals that the pigment glands are synthetic sites of gossypol in aerial parts of cotton

Zhang,

Long,

Zhao

et al. 2023

New Phytologist

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Summary Gossypol and the related terpenoids are stored in the pigment gland to protect cotton plants from biotic stresses, but little is known about the synthetic sites of these metabolites. Here, we showed that GoPGF, a key gene regulating gland formation, was expressed in gland cells and roots. The chromatin immunoprecipitation sequencing (ChIP‐seq) analysis demonstrated that GoPGF targets GhJUB1 to regulate gland morphogenesis. RNA‐sequencing (RNA‐seq) showed high accumulation of gossypol biosynthetic genes in gland cells. Moreover, integrated analysis of the ChIP‐seq and RNA‐seq data revealed that GoPGF binds to the promoter of several gossypol biosynthetic genes. The cotton callus overexpressing GoPGF had dramatically increased the gossypol levels, indicating that GoPGF can directly activate the biosynthesis of gossypol. In addition, the gopgf mutant analysis revealed the existence of both GoPGF‐dependent and ‐independent regulation of gossypol production in cotton roots. Our study revealed that the pigment glands are synthetic sites of gossypol in aerial parts of cotton and that GoPGF plays a dual role in regulating gland morphogenesis and gossypol biosynthesis. The study provides new insights for exploring the complex relationship between glands and the metabolites they store in cotton and other plant species.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 3 more Smart Citations

The dual role of GoPGF reveals that the pigment glands are synthetic sites of gossypol in aerial parts of cotton

Zhang,

Long,

Zhao

et al. 2023

New Phytologist

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Heavy Metals, Their Phytotoxicity, and the Role of Phenolic Antioxidants in Plant Stress Responses with Focus on Cadmium: Review

Goncharuk

Загоскина

2023

Molecules

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The current state of heavy metal (HM) environmental pollution problems was considered in the review: the effects of HMs on the vital activity of plants and the functioning of their antioxidant system, including phenolic antioxidants. The latter performs an important function in the distribution and binding of metals, as well as HM detoxification in the plant organism. Much attention was focused on cadmium (Cd) ions as one of the most toxic elements for plants. The data on the accumulation of HMs, including Cd in the soil, the entry into plants, and the effect on their various physiological and biochemical processes (photosynthesis, respiration, transpiration, and water regime) were analyzed. Some aspects of HMs, including Cd, inactivation in plant tissues, and cell compartments, are considered, as well as the functioning of various metabolic pathways at the stage of the stress reaction of plant cells under the action of pollutants. The data on the effect of HMs on the antioxidant system of plants, the accumulation of low molecular weight phenolic bioantioxidants, and their role as ligand inactivators were summarized. The issues of polyphenol biosynthesis regulation under cadmium stress were considered. Understanding the physiological and biochemical role of low molecular antioxidants of phenolic nature under metal-induced stress is important in assessing the effect/aftereffect of Cd on various plant objects—the producers of these secondary metabolites are widely used for the health saving of the world’s population. This review reflects the latest achievements in the field of studying the influence of HMs, including Cd, on various physiological and biochemical processes of the plant organism and enriches our knowledge about the multifunctional role of polyphenols, as one of the most common secondary metabolites, in the formation of plant resistance and adaptation.

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A Grafting Procedure for Gossypium hirsutum Based on In Vitro Grown Seedlings

Pandeya,

Campbell,

Rathore

2023

JCS

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Intervarietal grafting, and in some cases interspecific grafting, practiced mainly in horticultural crops has many practical applications. Grafting can also be a useful tool to perform basic physiological, biochemical, and molecular studies to better understand communication between shoot and root in a row crop, such as cotton. We have established a grafting protocol for upland cotton (Gossypium hirsutum L.) genotypes. Although less efficient, it can be used to create grafts between upland and Pima (G. barbadense) or Old-world cottons (G. arboreum or G. herbaceum). The procedure used 8-day-old seedlings grown in culture under sterile conditions to obtain the scion and rootstock. The scion consisted of the shoot apical meristem with a few leaf primordia, epicotyl, and a small portion of the hypocotyl with the cotyledons removed. The rootstock retained 2 to 3 cm of basal hypocotyl portion with the roots trimmed. Cleft grafts were assembled under aseptic conditions followed by their culture in vitro. Once established, as indicated by the healthy growth of three to four true leaves and roots, the grafts were moved to soil in small pots. After hardening the grafts on a laboratory bench for few weeks, they were transferred to larger pots and grown to maturity in a greenhouse to obtain seeds. The success rate using our protocol ranged from 90 to 100% for Coker 312 genotype and its derivative transgenic and gene edited lines. The method was also applicable to generate intervarietal and interspecific graft unions among various types of cotton plants.

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Gossypol and related compounds are produced and accumulate in the aboveground parts of the cotton plant, independent of roots as the source

Cited by 3 publications

References 61 publications

The dual role of GoPGF reveals that the pigment glands are synthetic sites of gossypol in aerial parts of cotton

The dual role of GoPGF reveals that the pigment glands are synthetic sites of gossypol in aerial parts of cotton

Heavy Metals, Their Phytotoxicity, and the Role of Phenolic Antioxidants in Plant Stress Responses with Focus on Cadmium: Review

A Grafting Procedure for Gossypium hirsutum Based on In Vitro Grown Seedlings

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