Göttingen Minipigs as Large Animal Model in Toxicology

Pedersen, Henrik D.; Mikkelsen, Lars Friis

doi:10.1016/b978-0-12-814655-2.00003-7

Cited by 9 publications

(6 citation statements)

References 73 publications

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“…Because of their similarities to humans in terms of genetics, anatomy, physiology, and biochemistry, minipigs are valuable animal models for drug testing and are being increasingly employed as non-rodent models for toxicity testing and safety pharmacology, 67 , 68 adding to the utility of our Göttingen minipig SORL1 het model in pharmacological research targeting AD.…”

Section: Discussionmentioning

confidence: 99%

A genetically modified minipig model for Alzheimer’s disease with SORL1 haploinsufficiency

Andersen¹,

Bøgh²,

Landau³

et al. 2022

Cell Reports Medicine

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Section: Discussionmentioning

confidence: 99%

A genetically modified minipig model for Alzheimer’s disease with SORL1 haploinsufficiency

Andersen¹,

Bøgh²,

Landau³

et al. 2022

Cell Reports Medicine

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“…The importance of swine and especially Ellegaard Göttingen Minipigs (EGMs) as a model in translational research and experimental medicine is steadily growing (1)(2)(3)(4)(5)(6)(7)(8)(9). In particular, they are increasingly recognized as valuable models for toxicity studies and xenograft research (10-13).…”

Section: Introductionmentioning

confidence: 99%

Characterization of the immune system of Ellegaard Göttingen Minipigs - An important large animal model in experimental medicine

et al. 2022

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Interest in Ellegaard Göttingen Minipigs (EGMs) as a model in experimental medicine is continuously growing. The aim of this project is to increase the knowledge of the immune system of EGMs as information is still scarce. Therefore, we studied the postnatal maturation of their immune system from birth until 126 weeks of age. For the first 26 weeks of the study, animals were kept under pathogen-reduced conditions (SPF) and afterwards under conventional housing conditions. The development of the immune system was analyzed by monitoring changes in total numbers of leukocytes and lymphocytes of ten individuals and the composition of leukocyte populations by multi-color flow cytometry (FCM). We followed the presence of monocytes using monoclonal antibodies (mAbs) against CD172a+ and CD163+ and B cells based on the expression of CD79a. NK cells were distinguished as CD3-CD16+CD8α+/dim cells and further subdivided using NKp46 (CD335) expression into NKp46-, NKp46+, and NKp46high NK cells. T-cell receptor (TCR) γδ T cells were defined by the expression of TCR-γδ and different subsets were determined by their CD2 and perforin expression. TCR-αβ T cells were classified by their CD8β+ or CD4 expression. For monitoring their differentiation, expression of CD27 and perforin was investigated for CD8β++ T cells and CD8α together with CD27 for CD4+ T cells. We clearly detected a postnatal development of immune cell composition and identified phenotypes indicative of differentiation within the respective leukocyte subsets. Examination of the development of the antigen-specific immune system after transfer to different distinct housing conditions and after vaccination against common porcine pathogens such as porcine circovirus 2 (PCV2) revealed a markedly increased presence of more differentiated CD8+ and CD4+ T cells with central and effector memory T-cell phenotypes. To complement the findings, a PCV2 vaccine-specific antigen was used for in vitro restimulation experiments. We demonstrated antigen-specific proliferation of CD4+CD8α+CD27+ central and CD4+CD8α+CD27- effector memory T cells as well as antigen-specific production of TNF-α and IFN-γ. This study of postnatal immune development defines basic cellular immune parameters of EGMs and represents an important milestone for the use of EGMs for immunological questions in experimental medicine.

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“…Also, genetically altered Göttingen Minipigs and humanised models have the potential to bridge the gap from in vivo to clinical studies in humans. 78,79…”

Section: Zebrafish Counterparts For Classical Non-clinical Safety Ass...mentioning

confidence: 99%

Advances in Animal Models and Cutting-Edge Research in Alternatives: Proceedings of the Second International Conference on 3Rs Research and Progress, Hyderabad, 2021

et al. 2022

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The fact that animal models fail to replicate human disease faithfully is now being widely accepted by researchers across the globe. As a result, they are exploring the use of alternatives to animal models. The time has come to refine our experimental practices, reduce the numbers and eventually replace the animals used in research with human-derived and human-relevant 3-D disease models. Oncoseek Bio-Acasta Health, which is an innovative biotechnology start-up company based in Hyderabad and Vishakhapatnam, India, organises an annual International Conference on 3Rs Research and Progress. In 2021, this conference was on ‘Advances in Research Animal Models and Cutting-Edge Research in Alternatives’. This annual conference is a platform that brings together eminent scientists and researchers from various parts of the world, to share recent advances from their research in the field of alternatives to animals including new approach methodologies, and to promote practices to help refine animal experiments where alternatives are not available. This report presents the proceedings of the conference, which was held in hybrid mode (i.e. virtual and in-person) in November 2021.

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Göttingen Minipigs as Large Animal Model in Toxicology

Cited by 9 publications

References 73 publications

A genetically modified minipig model for Alzheimer’s disease with SORL1 haploinsufficiency

A genetically modified minipig model for Alzheimer’s disease with SORL1 haploinsufficiency

Characterization of the immune system of Ellegaard Göttingen Minipigs - An important large animal model in experimental medicine

Advances in Animal Models and Cutting-Edge Research in Alternatives: Proceedings of the Second International Conference on 3Rs Research and Progress, Hyderabad, 2021

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