Göttingen Minipigs in Ocular Research

Shrader, Stephanie; Greentree, William F

doi:10.1177/0192623318770379

Cited by 30 publications

(24 citation statements)

References 15 publications

(14 reference statements)

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“…When designing ocular studies and analyzing the resultant data, it is, therefore, imperative to understand the basic normative ocular anatomy of the animal model being considered. Although Göttingen minipigs are increasingly utilized for ocular toxicology and biocompatibility research (previously reviewed), to the authors’ knowledge, basic anatomic histomorphometric data for Göttingen minipig eyes has not previously been published.…”

Section: Discussionmentioning

confidence: 99%

Histomorphometric evaluation of the Göttingen minipig eye

Shrader

Mowry

2019

Veterinary Ophthalmology

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Objective:The Göttingen minipig is becoming a popular nonrodent animal model in ocular research; however, there is a paucity of literature regarding normative ocular reference data in this breed. We, therefore, investigated the characteristics of the cornea, retina, and sclera in order to establish baseline histomorphometric data in male and female Göttingen minipigs. Procedures: This study utilized paraffin-embedded, Davidson's-fixed, control Göttingen minipig eyes (six males and eight females). Hematoxylin and eosin stained slides of the eyes were scanned via an Aperio slide scanner and analyzed using Aperio ImageScope™. Linear measurements were made of the cornea, retina, and sclera. Results: There were no statistically significant differences between males and females in corneal or scleral measurements or total retinal thickness. There were, however, statistically significant differences between the sexes in the thickness of the ventral peripheral ganglion cell layer [13.15 µm (±3.65) in males; 10.68 µm (±3.37) in females; P = 0.03], ventral peripheral inner plexiform layer [23.47 µm (±2.85) in males; 21.16 µm (±3.62) in females; P = 0.03], ventral central outer plexiform layer [7.97 µm (±2.43) in males; 6.63 µm (±1.73) in females; P = 0.02], and ventral peripheral outer plexiform layer [8.79 µm (±1.82) in males; 11.23 µm (±3.11) in females; P = 0.01]. Conclusions: This study provides normative histomorphometric reference data for the Göttingen minipig eye. These data will aid researchers in study design and interpretation of findings in Göttingen minipig ocular studies. K E Y W O R D S eye, eye pathology, Göttingen minipig, morphometry, ocular histomorphometry, ocular toxicology How to cite this article: Shrader SM, Mowry RN. Histomorphometric evaluation of the Göttingen minipig eye. Vet Ophthalmol. 2019;22:872-878. https://doi.

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Section: Discussionmentioning

confidence: 99%

Histomorphometric evaluation of the Göttingen minipig eye

Shrader

Mowry

2019

Veterinary Ophthalmology

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“…32 Gottingen, Yucatan, Hanford, and Sinclair minipigs have been used in research settings, and the smallest of these minipig models is the Gottingen, which has rapidly gained popularity among researchers for ocular studies, including those investigating ocular diseases, novel therapeutics, surgical techniques, and implantable materials/devices. 32 Other strains have successfully been used for ocular toxicity studies, which may be CRO or institutional preference. The author is not aware of advantages or disadvantages of particular strains other than the size advantage and greater amount of literature available on Göttingen minpigs.…”

Section: Minipig Species Selection Considerationsmentioning

confidence: 99%

Selected Aspects of Ocular Toxicity Studies With a Focus on High-Quality Pathology Reports: A Pathology/Toxicology Consultant’s Perspective

Short¹

2020

Toxicol Pathol

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Ocular toxicity studies are the bedrock of nonclinical ocular drug and drug–device development, and there has been an evolution in experience, technologies, and challenges to address that ensures safe clinical trials and marketing authorization. The expectations of a well-designed ocular toxicity study and the generation of a coherent, integrative ocular toxicology report and subreports are high, and this article provides a pathology/toxicology consultant’s perspective on achieving that goal. The first objective is to cover selected aspects of study designs for ocular toxicity studies including considerations for contract research organization selection, minipig species selection, unilateral versus bilateral dosing, and in-life parameters based on fit-for-purpose study objectives. The main objective is a focus on a high-quality ocular pathology report that includes ocular histology procedures to meet regulatory expectations and a report narrative and tables that correlate microscopic findings with key ophthalmic findings and presents a clear interpretation of test article-, vehicle-, and procedure-related ocular and extraocular findings with identification of adversity and a pathology peer review. The last objective covers considerations for a high-quality ophthalmology report, which in concert with a high-quality pathology report, will pave the way for a best quality toxicology report for an ocular toxicity study.

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“…However, the porcine species (either domestic pigs or minipigs) are increasingly used in view of the closer resemblance with the human eye. Useful reviews list the different animal models for ocular studies and help in choosing the most appropriate species (Shrader and Greentree 2018; Tsonis 2008).…”

Section: Animal Models For the Evaluation Of Ocular Mdsmentioning

confidence: 99%

Ocular Medical Devices: Histologic Technique and Histopathologic Evaluation of the Biocompatibility and Performance

Drevon-Gaillot

2018

Toxicol Pathol

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Ocular medical devices (MDs) represent a very wide and promising field of human ophthalmology. In preclinical studies evaluating the safety and/or performance of these ocular MDs, the choice of histologic technique and the focus of the histopathologic evaluation method should take into consideration the following aspects: the specific guidelines possibly associated with the MD or combination product, the ocular compartment in contact with the MD and its specificities, and last the nature of the biomaterial used in the MD. Following a brief overview of animal models, this short review will present the different types of ocular MDs and will present the specificities of the histologic technique and the histopathologic evaluation related to ocular MDs.

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Göttingen Minipigs in Ocular Research

Cited by 30 publications

References 15 publications

Histomorphometric evaluation of the Göttingen minipig eye

Histomorphometric evaluation of the Göttingen minipig eye

Selected Aspects of Ocular Toxicity Studies With a Focus on High-Quality Pathology Reports: A Pathology/Toxicology Consultant’s Perspective

Ocular Medical Devices: Histologic Technique and Histopathologic Evaluation of the Biocompatibility and Performance

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