Uganda’s aquaculture sub-sector is rapidly developing, contributing up to 22% of all fish produced in the country. However, the sub-sector still faces challenges related to limited access to extension services, production inputs, post-harvest handling facilities, disease incidences, value-addition skills and weak market linkages. Although there have been isolated interventions at different aquaculture value-chain nodes, the registered challenges continue to recur. To address prioritized issues in Uganda’s aquaculture sub-sector, nine zonal aquaculture innovation platforms were established based on the country’s nine agro-ecological zones. These platforms brought together different stakeholders to identify solutions to common problems in each aquaculture value-chain node. Each innovation platform consisted of fish farmers, hatchery operators, traders, extension workers, researchers, input dealers, local leaders, and financial institutions. During a series of zonal workshops, participants identified constraints to the aquaculture sub-sector in their respective zones. From the findings, there are technical knowledge gaps across the entire aquaculture value chain mainly affecting hatchery operators, input suppliers, and farmers. Therefore, there is need for coordinated interventions to enhance access to production technologies, innovations, knowledge, and skill transfer to promote gender-centered aquaculture development.