gp13 (EHV-gC): a complement receptor induced by equine herpesviruses

Huemer, Hartwig P.; Nowotny, Norbert; Crabb, Brendan S.; Meyer, Hermann; Hübert, P.

doi:10.1016/0168-1702(95)00027-n

Cited by 33 publications

(15 citation statements)

References 34 publications

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“…PRV, BHV-1, EHV-1 and EHV-4 gC orthologues are known to bind C3b of the complement cascade (with highest affinity to C3 of the natural host), presumably resulting in inhibition of further downstream events (Huemer et al, 1992(Huemer et al, , 1993(Huemer et al, , 1995.…”

Section: Herpesviridaementioning

confidence: 99%

Virus complement evasion strategies

Favoreel

Walle

Nauwynck

et al. 2003

Journal of General Virology

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The immune system has a variety of tools at its disposal to combat virus infections. These can be subdivided roughly into two categories: 'first line defence', consisting of the non-specific, innate immune system, and 'adaptive immune response', acquired over time following virus infection or vaccination. During evolution, viruses have developed numerous, and often very ingenious, strategies to counteract efficient recognition of virions or virus-infected cells by both innate and adaptive immunity. This review will focus on the different strategies that viruses use to avoid recognition by one of the components of the immune system: the complement system. Complement evasion is of particular importance for viruses, since complement activation is a crucial component of innate immunity (alternative and mannan-binding lectin activation pathway) as well as of adaptive immunity (classical, anti body-dependent complement activation)

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Section: Herpesviridaementioning

confidence: 99%

Virus complement evasion strategies

Favoreel

Walle

Nauwynck

et al. 2003

Journal of General Virology

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“…For comparison, similar expression plasmids were constructed, inserting the full-length gC genes from EHV-1 (Huemer et al, 1995) and Marek's disease virus (MDV, kindly provided by K. Osterrrieder, IMB/BFAV, Insel Riems, Germany) into pCR3.1.…”

Section: Methodsmentioning

confidence: 99%

“…Production of antisera and monoclonal antibodies against gC HSV-1 has been described previously (Huemer et al, 1989). Monoclonal and polyclonal antibodies against gC homologues from PRV, bovine herpesvirus type 1 (BHV-1) and EHV-1 have been used as described earlier (Huemer et al, 1992b(Huemer et al, , 1993a(Huemer et al, , 1995. Antisera were tested by immunofluoresence using 12-well microscopic slides coated with acetone/methanol-fixed SHBV-infected Vero or RK13 cells stably transfected with gC SHBV or control constructs.…”

Section: Methodsmentioning

confidence: 99%

“…Restriction digests were separated on 1 % agarose, blotted onto nylon membranes and hybridized with gC HSV-1 -and gC EHV-1 -specific probes under conditions of low stringency (50˚C, 0?5 % SSC wash). As probes, restriction fragments were excised from plasmids containing the coding sequences of HSV-1 strain KOS (Huemer et al, 1989) and EHV-1 strain Piber (Huemer et al, 1995) and labelled by random priming using Klenow polymerase and digoxigenin-labelled UTP. Blots were developed using the anti-digoxigenin system from Boehringer Mannheim.…”

Section: Methodsmentioning

confidence: 99%

“…This has been found previously to be useful for detection of complement receptors expressed by HSV-1, EHV-1, EHV-4 and a guinea pig herpesvirus, but not for HSV-2, BHV-1 or PRV (Huemer et al, 1992a(Huemer et al, , 1993a(Huemer et al, , 1995. Inhibition of SRBC binding of SHBV-infected cells was performed using heparin sulphate, dextran sulphate and other polysulphates under conditions identical to those described for HSV-1 (Huemer et al, 1992a).…”

Section: Radioimmunoprecipitation Of Vaccinia Virus Lysates Was Perfomentioning

confidence: 99%

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Cloning and expression of the complement receptor glycoprotein C from Herpesvirus simiae (herpes B virus): protection from complement-mediated cell lysis

Huemer

Wechselberger

Bennett

et al. 2003

Journal of General Virology

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Simian herpes B virus (SHBV) is the herpes simplex virus (HSV) homologue for the speciesMacaca. Unlike in its natural host, and unlike other animal herpesviruses, SHBV causes high mortality in accidentally infected humans. SHBV-infected cells, like those infected with HSV-1 and equine herpesvirus types 1 and 4, express complement C3 receptor activity. To study immunoregulatory functions involved in susceptibility/resistance against interspecies transmission, the SHBV glycoprotein C (gC SHBV ) gene (encoding 467 aa) was isolated. Sequence analysis revealed amino acid identity with gC proteins from HSV-2 (46?9 %), HSV-1 (44?5 %) and pseudorabies virus (21?2 %). Highly conserved cysteine residues were also noted. Similar to gC HSV-2 , gC SHBV is less glycosylated than gC HSV-1 , resulting in a molecular mass of 65 kDa if expressed in replication-deficient vaccinia virus Ankara. Stable transfectants expressing full-length gC SHBV on the cell surface induced C3 receptor activity and were substantially protected from complement-mediated lysis; no protection was observed with control constructs. This suggests that expression of the gC homologues on infected cell surfaces might also contribute to the survival of infected cells in addition to decreased virion inactivation. Interestingly, soluble gC SHBV isolated from protein-free culture supernatants did not interfere with the binding of the alternative complement pathway activator properdin to C3b, which is similar to our findings with gC HSV-2 and could be attributed to major differences in the amino-terminal portion of the protein with extended deletions in both gC SHBV and gC HSV-2 . Binding of recombinant gC SHBV to polysulphates was observed. This, together with the heparin-sensitivity of the gC SHBV -C3 interaction on the infected cell surface, suggests a role in adherence to heparan sulphate, similar to the gC proteins of other herpesviruses.

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Viral Heparin-Binding Complement Inhibitors – A Recurring Theme

Blom

Mark

Spiller

Advances in Experimental Medicine and Biology

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gp13 (EHV-gC): a complement receptor induced by equine herpesviruses

Cited by 33 publications

References 34 publications

Virus complement evasion strategies

Virus complement evasion strategies

Cloning and expression of the complement receptor glycoprotein C from Herpesvirus simiae (herpes B virus): protection from complement-mediated cell lysis

Viral Heparin-Binding Complement Inhibitors – A Recurring Theme

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