Gp130‐specific Antisense Oligonucleotides Inhibit Il‐6 Signal Inducing Junb Mrna Transcription in the Human Hepatoma Cell Line, Hepg2

Abstract

The biosynthesis of interleukin-6 receptor (IL-6R) and gp130 in vitro was blocked using specific antisense oligonucleotides (ASO) in HepG2 liver cells and the efficacy of various ASOs was tested on the generation of IL-6-induced junB mRNA. We used three ASOs specific for the IL-6 receptor, three specific for gp130 and a control (nonsense) oligonucleotide specific for epsilon-chain of IgE (not expressing in HepG2 cells). Our data indicate that a gp130-specific ASO, g2, was the most effective blocker of IL-6-induced junB mRNA, whilst the IL-6 receptor ASOs alone were ineffective. The mechanism of gene inactivation by ASO treatment was partially elucidated by demonstration of the loss of gp130 mRNA from cells treated with ASOs showing functional efficacy. Our data may help to design antisense oligonucleotides that are effective in therapy (e.g. as anti-inflammatory agents) in the future.

Retinal Pigment Epithelial Cell Behavior is Modulated by Alterations in Focal Cell–Substrate Contacts

“…The mechanism by which the RPE cells undergo changes in focal contact assembly into different biochemical responses remains unclear and requires further investigation. 45,46 However, these results provided strong evidence to support the previous findings that substrate surface topography alters cell shape and . Bottom: A percentage plot of the BrdU-labeled ARPE-19 cells indicated that the cell cycles were significantly inhibited on the micropatterned surfaces.…”

Mesothelin Overexpression Promotes Autocrine IL-6/sIL-6R Trans-Signaling to Stimulate Pancreatic Cancer Cell Proliferation

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