Gp66, a calcineurin family phosphatase encoded by mycobacteriophage D29, is a 2′, 3′ cyclic nucleotide phosphodiesterase that negatively regulates phage growth

Dutta, Soumita; Bhawsinghka, Niketa; Gupta, S. K.

doi:10.1111/1574-6968.12625

Cited by 4 publications

(3 citation statements)

References 23 publications

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“…A set of AMGs were identified from group I and II genomes, including genes encoding MPEs, amidoligase, GATase, 2OG-Fe(II) oxygenase, SufA, and Sulfotransferse. MPEs exhibit hydrolase activity against a wide range of phosphorylated substrates and are common in bacterial and archaeal genomes ( 54 – 56 ). Phage-encoded MPEs have been identified in many phage genomes ( 56 , 57 ).…”

Section: Discussionmentioning

confidence: 99%

“…MPEs exhibit hydrolase activity against a wide range of phosphorylated substrates and are common in bacterial and archaeal genomes ( 54 – 56 ). Phage-encoded MPEs have been identified in many phage genomes ( 56 , 57 ). A previous study has suggested that Mycobacterium virus D29-encoded MPEs can negatively regulate the growth of bacteriophages and bacteria ( 56 ).…”

Section: Discussionmentioning

confidence: 99%

“…Phage-encoded MPEs have been identified in many phage genomes ( 56 , 57 ). A previous study has suggested that Mycobacterium virus D29-encoded MPEs can negatively regulate the growth of bacteriophages and bacteria ( 56 ). The function of phage-encoded MPEs in OM43 phages requires further investigation.…”

Section: Discussionmentioning

confidence: 99%

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Genomic diversity and biogeographic distributions of a novel lineage of bacteriophages that infect marine OM43 bacteria

Yang,

Du,

Zhang

et al. 2023

Microbiol Spectr

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The marine methylotrophic OM43 clade is considered an important bacterial group in coastal microbial communities. OM43 bacteria, which are closely related to phytoplankton blooms, have small cell sizes and streamlined genomes. Bacteriophages profoundly shape the evolutionary trajectories, population dynamics, and physiology of microbes. The prevalence and diversity of several phages that infect OM43 bacteria have been reported. In this study, we isolated and sequenced two novel OM43 phages, MEP401 and MEP402. These phages share 90% of their open reading frames (ORFs) and are distinct from other known phage isolates. Furthermore, a total of 99 metagenomic viral genomes (MVGs) closely related to MEP401 and MEP402 were identified. Phylogenomic analyses suggest that MEP401, MEP402, and these identified MVGs belong to a novel subfamily in the family Zobellviridae and that they can be separated into two groups. Group I MVGs show conserved whole-genome synteny with MEP401, while group II MVGs possess the MEP401-type DNA replication module and a distinct type of morphogenesis and packaging module, suggesting that genomic recombination occurred between phages. Most members in these two groups were predicted to infect OM43 bacteria. Metagenomic read-mapping analysis revealed that the phages in these two groups are globally ubiquitous and display distinct biogeographic distributions, with some phages being predominant in cold regions, some exclusively detected in estuarine stations, and others displaying wider distributions. This study expands our knowledge of the diversity and ecology of a novel phage lineage that infects OM43 bacteria by describing their genomic diversity and global distribution patterns. IMPORTANCE OM43 phages that infect marine OM43 bacteria are important for host mortality, community structure, and physiological functions. In this study, two OM43 phages were isolated and characterized. Metagenomic viral genome (MVG) retrieval using these two OM43 phages as baits led to the identification of two phage groups of a new subfamily in the family Zobellviridae . We found that group I MVGs share similar genomic content and arrangement with MEP401 and MEP402, whereas group II MVGs only possess the MEP401-type DNA replication module. Metagenomic mapping analysis suggests that members in these two groups are globally ubiquitous with distinct distribution patterns. This study provides important insights into the genomic diversity and biogeography of the OM43 phages in the global ocean.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Genomic diversity and biogeographic distributions of a novel lineage of bacteriophages that infect marine OM43 bacteria

Yang,

Du,

Zhang

et al. 2023

Microbiol Spectr

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Biological and molecular characterization of fEg-Eco19, a lytic bacteriophage active against an antibiotic-resistant clinical Escherichia coli isolate

et al. 2022

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Characterization of bacteriophages facilitates better understanding of their biology, host specificity, genomic diversity, and adaptation to their bacterial hosts. This, in turn, is important for the exploitation of phages for therapeutic purposes, as the use of uncharacterized phages may lead to treatment failure. The present study describes the isolation and characterization of a bacteriophage effective against the important clinical pathogen Escherichia coli, which shows increasing accumulation of antibiotic resistance. Phage fEg-Eco19, which is specific for a clinical E. coli strain, was isolated from an Egyptian sewage sample. Phage fEg-Eco19 formed clear, sharp-edged, round plaques. Electron microscopy showed that the isolated phage is tailed and therefore belongs to the order Caudovirales, and morphologically, it resembles siphoviruses. The diameter of the icosahedral head of fEg-Eco19 is 68 ± 2 nm, and the non-contractile tail length and diameter are 118 ± 0.2 and 13 ± 0.6 nm, respectively. The host range of the phage was found to be narrow, as it infected only two out of 137 clinical E. coli strains tested. The phage genome is 45,805 bp in length with a GC content of 50.3% and contains 76 predicted genes. Comparison of predicted and experimental restriction digestion patterns allowed rough mapping of the physical ends of the phage genome, which was confirmed using the PhageTerm tool. Annotation of the predicted genes revealed gene products belonging to several functional groups, including regulatory proteins, DNA packaging and phage structural proteins, host lysis proteins, and proteins involved in DNA/RNA metabolism and replication.

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Application of a novel phage ZPAH7 for controlling multidrug-resistant Aeromonas hydrophila on lettuce and reducing biofilms

et al. 2021

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Gp66, a calcineurin family phosphatase encoded by mycobacteriophage D29, is a 2′, 3′ cyclic nucleotide phosphodiesterase that negatively regulates phage growth

Cited by 4 publications

References 23 publications

Genomic diversity and biogeographic distributions of a novel lineage of bacteriophages that infect marine OM43 bacteria

Genomic diversity and biogeographic distributions of a novel lineage of bacteriophages that infect marine OM43 bacteria

Biological and molecular characterization of fEg-Eco19, a lytic bacteriophage active against an antibiotic-resistant clinical Escherichia coli isolate

Application of a novel phage ZPAH7 for controlling multidrug-resistant Aeromonas hydrophila on lettuce and reducing biofilms

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