GPCRs that Rhoar the Guanine nucleotide exchange factors

Quantitative proteomics reveals CLR interactome in primary human cells

“…2 C ) interact with a larger network of proteins than it was previously described in studies using receptor overexpression models. This network includes a distinct subset of molecules that play roles in GPCR biology, such as specific guanine nucleotide exchange factors ( 55 ), chaperones ( 56 ), ubiquitin-specific proteases ( 57 ), nucleoporins ( 58 ), ER calcium ATPases ( 59 ), clathrin ( 60 ) and calcium/calmodulin-dependent protein kinases ( 61 ) ( Fig. 6 , B and C ; Table S5 ).…”

Dishevelled2 activates WGEF via its interaction with a unique internal peptide motif of the GEF

“…This switching between ON and OFF states is brought about by the regulatory proteins known as Guanine nucleotide exchange factors (GEFs) and GTPase activating proteins (GAPs), respectively 9 . Appropriate signalling cues, transducing from the membrane receptors such as G-protein coupled receptors (GPCRs) 10 , receptor tyrosine kinase 11 and integrin receptors 12 , activate their downstream GEFs, which in turn trigger Rho GTPases by exchanging their bound GDP with GTP. However, the majority of GEFs exist in an autoinhibitory state and require other signalling proteins for their activation 10 .…”

“…Appropriate signalling cues, transducing from the membrane receptors such as G-protein coupled receptors (GPCRs) 10 , receptor tyrosine kinase 11 and integrin receptors 12 , activate their downstream GEFs, which in turn trigger Rho GTPases by exchanging their bound GDP with GTP. However, the majority of GEFs exist in an autoinhibitory state and require other signalling proteins for their activation 10 . In the context of Wnt-PCP pathway WGEF (Weakly similar to RhoGEF 5/TIM), also known as Arhgef19/ Ephexin2), activates RhoA 13 , 14 .…”