GPI-AP release in cellular, developmental, and reproductive biology

Fujihara, Yoshitaka; Ikawa, Masahito

doi:10.1194/jlr.r063032

Cited by 62 publications

(65 citation statements)

References 111 publications

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“…odr-2 encodes a novel protein distantly related to murine Ly6 lymphocyte antigen protein that is tethered to membrane by a GPI (glycosylphosphatidylinositol) anchor [19]. In vertebrates, GPI anchored proteins have roles in development and neurogenesis by regulating several signaling pathways including Notch, Wnt or TGFβ [33]. Moreover, a set of Ly6 related genes including Lynx1 and Lynx2 have been shown to be expressed in the nervous system and appear to be accessory components of nicotinic acetylcholine receptors, thus modulating their functions [34, 35].…”

Section: Discussionmentioning

confidence: 99%

Early Pheromone Experience Modifies a Synaptic Activity to Influence Adult Pheromone Responses of C. elegans

Hong

Ryu

et al. 2017

Current Biology

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SUMMARY Experiences during early development can influence neuronal functions and modulate adult behaviors [1, 2]. However, the molecular mechanisms underlying the long-term behavioral effects of these early experiences are not fully understood. The C. elegans ascr#3 (asc-0394C9, C9) pheromone triggers avoidance behavior in adult hermaphrodites [3–7]. Here, we show that hermaphrodites that are briefly exposed to ascr#3 immediately after birth exhibit increased ascr#3-specific avoidance as adults indicating that ascr#3-experienced animals form a long lasting memory or imprint of this early ascr#3 exposure [8]. Ascr#3 imprinting is mediated by increased synaptic activity between the ascr#3-sensing ADL neurons and their post-synaptic SMB motor neuron partners via increased expression of the odr-2 GPI-linked signaling gene in the SMB neurons. Our study suggests that the memory for early ascr#3 experience is imprinted via alteration of activity of a single synaptic connection, that in turn shapes experience-dependent plasticity in adult ascr#3 responses.

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Section: Discussionmentioning

confidence: 99%

Early Pheromone Experience Modifies a Synaptic Activity to Influence Adult Pheromone Responses of C. elegans

Hong

Ryu

et al. 2017

Current Biology

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“…As mentioned above, CEACAM6 is bound to the plasma membrane through a GPI anchor. Such anchors are synthesized in the endoplasmic reticulum . The eukaryotic GPI anchor consists of a backbone of N‐acetyl‐glucosamine, 3 mannose residues, and ethanolamine bound to a phosphoinositol molecule covalently linked to a glycerolipid moiety located within the outer leaflet of the plasma membrane.…”

Section: Structure Of Carcinoembryonic Antigen/carcinoembryonic Antigmentioning

confidence: 99%

Towards understanding the mechanisms of actions of carcinoembryonic antigen‐related cell adhesion molecule 6 in cancer progression

2018

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Human carcinoembryonic antigen (CEA) is the prototypic member of a family of highly related cell surface glycoproteins that includes carcinoembryonic antigen‐related cell adhesion molecule 6 (CEACAM6) and others. CEACAM6 (formerly NCA), which belongs to the immunoglobulin superfamily, is a cell adhesion protein of the CEA family. It is normally expressed on the epithelial surfaces and on the surface of myeloid cells (CD66c). CEACAM6 is a multi‐functional glycoprotein that mediates homotypic binding with other CEA family members and heterotypic binding with integrin receptors. It functions by organizing tissue architecture and regulating different signal transduction, while aberrant expression leads to the development of human malignancies. It was first discovered in proliferating cells of adenomas and hyperplastic polyps in comparison to benign colonic tissue when overexpressed on the surface of various cell types in model systems. CEACAM6 functions as a pan‐inhibitor of cell differentiation and cell polarization, and it also causes distortion of tissue architecture. Moreover, overexpression of CEACAM6 modulates cancer progression through aberrant cell differentiation, anti‐apoptosis, cell growth and resistance to therapeutic agents. In addition, CEACAM6 overexpression in multiple malignancies promotes cell invasion and metastasis, thereby representing an acquired advantage of tumor cells directly responsible for an invasive phenotype. This review focuses on the findings supporting the mechanisms of actions linking the oncogenic potential of CEACAM6 to the onset of cancer progression and pathogenesis, especially in breast cancer, and to validating CEACAM6 as a target to pave the way towards the design of efficient therapeutic strategies against breast cancer.

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“…Sequences analysis indicated that Tm GP50 has high similarity to Ts GP50 at the DNA and amino acid levels. Immunofluorescence showed that the Tm GP50 protein was highly localized to the microthrix and parenchymatous zone of both the adult parasite and the coenurus, as well as being widely distributed in the cystic wall of the coenurus, which is in agreement with the characteristics of GPI-APs [9–11]. …”

Section: Discussionmentioning

confidence: 71%

“…Glycosylphosphatidylinositol-anchored proteins (GPI-APs) are attached to the plasma membrane by a glycosylphosphatidylinositol anchor [9–11]. GPI-APs are structurally and functionally diverse and play vital roles in numerous biological processes [9].…”

Section: Introductionmentioning

confidence: 99%

GP50 as a promising early diagnostic antigen for Taenia multiceps infection in goats by indirect ELISA

Huang

Wang

et al. 2016

Parasites Vectors

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BackgroundCoenurosis is caused by coenurus, the metacestode of Taenia multiceps, which mainly parasitizes the brain and spinal cord of cattle, sheep and goats. To date, no widely-approved methods are available to identify early coenurus infection.MethodsIn this study, we identified a full-length cDNA that encodes GP50 (TmGP50) from the transcriptome of T. multiceps, and then cloned and expressed in E. coli. The native proteins in adult stage and coenurus were located via immunofluorescence assays, while the potential of recombinant TmGP50 protein (rTmGP50) for indirect ELISA-based serodiagnostics was assessed using native goat sera. In addition, we orally infected 20 goats with mature T. multiceps eggs. Praziquantel (10%) was given to 10 of the goats 45 days post-infection (p.i.). Blood samples were collected for 17 weeks p.i. from the 20 goats and anti-rTmGP50 antibodies were evaluated using the indirect ELISA established here.ResultsThe TmGP50 contains an 897 bp open reading frame, in which signal sequence resides in 1 ~ 48 sites and mature polypeptide consists of 282 amino acid residues. Immunofluorescence staining showed that native TmGP50 was localized to the microthrix and parenchymatous zone of the adult parasite and coenurus, and the coenurus cystic wall. The indirect ELISA based on rTmGP50 exhibited a sensitivity of 95.0% and a specificity of 92.6% when detecting GP50 antibodies in sera of naturally infected goats and sheep. In goats experimentally infected with T. multiceps, anti-TmGP50 antibody was detectable from 2 to 17 weeks p.i. in the control group, while the antibody fell below the cut-off value about 3 weeks after praziquantel treatment.ConclusionOur results indicate that recombinant TmGP50 is a suitable early diagnostic antigen for coenurus infection in goats.

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GPI-AP release in cellular, developmental, and reproductive biology

Cited by 62 publications

References 111 publications

Early Pheromone Experience Modifies a Synaptic Activity to Influence Adult Pheromone Responses of C. elegans

Early Pheromone Experience Modifies a Synaptic Activity to Influence Adult Pheromone Responses of C. elegans

Towards understanding the mechanisms of actions of carcinoembryonic antigen‐related cell adhesion molecule 6 in cancer progression

GP50 as a promising early diagnostic antigen for Taenia multiceps infection in goats by indirect ELISA

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