2010
DOI: 10.1111/j.1476-5381.2010.00744.x
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GPR55‐dependent and ‐independent ion signalling in response to lysophosphatidylinositol in endothelial cells

Abstract: Background and purposeThe glycerol-based lysophospholipid lysophosphatidylinositol (LPI) is an endogenous agonist of the G-protein-coupled receptor 55 (GPR55) exhibiting cannabinoid receptor-like properties in endothelial cells. To estimate the contribution of GPR55 to the physiological effects of LPI, the GPR55-dependent and -independent electrical responses in this cell type were investigated.Experimental approachApplying small interference RNA-mediated knock-down and transient overexpression, GPR55-dependen… Show more

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Cited by 62 publications
(69 citation statements)
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References 55 publications
(96 reference statements)
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“…Our observation that LPI causes a nonspecific Ca 2ϩ response in HEK293aeq cells in the absence of GPR55 and the GPR55-independent effects of LPI on ion channels (Bondarenko et al, 2010) emphasize the importance of non-LPI, noncannabinoid tools, to study GPR55. To begin to identify such tools, we investigated the structure-activity relationship of CP55,940, testing several close structural analogs previously described as CB 1 agonists (Little et al, 1988).…”
Section: Discussionmentioning
confidence: 99%
See 1 more Smart Citation
“…Our observation that LPI causes a nonspecific Ca 2ϩ response in HEK293aeq cells in the absence of GPR55 and the GPR55-independent effects of LPI on ion channels (Bondarenko et al, 2010) emphasize the importance of non-LPI, noncannabinoid tools, to study GPR55. To begin to identify such tools, we investigated the structure-activity relationship of CP55,940, testing several close structural analogs previously described as CB 1 agonists (Little et al, 1988).…”
Section: Discussionmentioning
confidence: 99%
“…LPI also activates ERK in microglial-derived BV-2 cells (Pietr et al, 2009) and induces intracellular Ca 2ϩ release in endothelial-derived EA.hy926 cells and in primary murine dorsal root ganglion cells (Lauckner et al, 2008;Waldeck-Weiermair et al, 2008). Recently, GPR55-independent effects of LPI have also been characterized, including activation of large conductance, Ca 2ϩ -activated K ϩ channels (BK Ca ), and other ion channels (Bondarenko et al, 2010).…”
Section: Discussionmentioning
confidence: 99%
“…4028) as an endogenous agonist (Oka et al, 2007). The activation of GPR55 by LPI has been described by numerous groups (Lauckner et al, 2008;Waldeck-Weiermair et al, 2008;Henstridge et al, 2009Henstridge et al, , 2010Kapur et al, 2009;Whyte et al, 2009;Yin et al, 2009;Bondarenko et al, 2010;Ford et al, 2010;Oka et al, 2010a;Ishiguro et al, 2011;Pineiro et al, 2011;Southern et al, 2013). Commercially available LPI is primarily plant-derived, containing mixtures of fatty acid substituents with a majority of mediumchain saturated and monounsaturated fatty acids at the 1-position of LPI (Oka et al, 2009).…”
Section: -(S)-hydroxyeicosatetraenoic Acid [12-(s)-hete;mentioning
confidence: 99%
“…The biologic activity of 2-arachidonoyl LPI was markedly higher than those of other molecular species of LPI tested as activators of extracellular signal-regulated kinase (ERK) or elevation of intracellular calcium ions in heterologous expression studies of GPR55 (Oka et al, 2009). It should be noted, however, that LPI has actions independent of GPR55, in inhibiting the plasma membrane Na + /K + -ATPase (Bondarenko et al, 2010) and regulating endothelial cell BK Ca channels, allowing an enhancement of activity at times when the channel activity was low and reducing channel activity at higher levels of channel stimulation (Bondarenko et al, 2010). The nomenclature of GPR55 is currently under consideration by the cannabinoid receptor subcommittee of NC-IUPHAR.…”
Section: -(S)-hydroxyeicosatetraenoic Acid [12-(s)-hete;mentioning
confidence: 99%
“…Studies in mice lacking GPR55 have reported a reduction in inflammatory and neuropathic pain (1), and these mice have increased bone mass (2). Subsequently, studies have established that the endogenous lysophospholipid LPI 2 activates GPR55 (3)(4)(5)(6)(7)(8)(9)(10). In certain cancer cell lines, GPR55 is highly expressed, and LPI mediates increased cell migration, invasion, and proliferation (3,8,11).…”
mentioning
confidence: 99%