GPX8 as a Novel Prognostic Factor and Potential Therapeutic Target in Primary Glioma

Yang, Zhaoshou; Qin, Y.; Sun, Xiaoxiao; Xiong, Ke; Zhu, Xiaoting; Wang, Yichen; Ren, Qian-yao; Wu, Guohui; Shi-min, Wang; Cao, Xiaoling; Yang, Xiao-rong; Jiang, Wengong

doi:10.1155/2022/8025055

Cited by 6 publications

(4 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In fact, GPx-8 is similar to GPx-7 in that both have low glutathione peroxidase activity while having a function related to promoting the oxidative folding of endoplasmic reticulum proteins and reducing oxidative stress [ 76 ]. It contains an N-terminal signal peptide and a C-terminal endoplasmic reticulum membrane localization signal and functions in regulating calcium homeostasis [ 77 , 78 ]. Importantly, the overexpression of GPx-8 reduces histamine-induced Ca 2+ storage and release in the endoplasmic reticulum [ 77 ]; thus, GPx-8 overexpression results in decreased Ca 2+ levels in the endoplasmic reticulum while its silencing causes Ca 2+ release from the endoplasmic reticulum to the mitochondria and cytoplasm [ 77 ].…”

Section: Discussionmentioning

confidence: 99%

Glutathione Peroxidase gpx1 to gpx8 Genes Expression in Experimental Brain Tumors Reveals Gender-Dependent Patterns

Cueto-Ureña,

Ramírez-Expósito,

Mayas

et al. 2023

Genes

View full text Add to dashboard Cite

Extensive research efforts in the field of brain tumor studies have led to the reclassification of tumors by the World Health Organization (WHO) and the identification of various molecular subtypes, aimed at enhancing diagnosis and treatment strategies. However, the quest for biomarkers that can provide a deeper understanding of tumor development mechanisms, particularly in the case of gliomas, remains imperative due to their persistently incurable nature. Oxidative stress has been widely recognized as a key mechanism contributing to the formation and progression of malignant tumors, with imbalances in antioxidant defense systems being one of the underlying causes for the excess production of reactive oxygen species (ROS) implicated in tumor initiation. In this study, we investigated the gene expression patterns of the eight known isoforms of glutathione peroxidase (GPx) in brain tissue obtained from male and female control rats, as well as rats with transplacental ethyl nitrosourea (ENU)-induced brain tumors. Employing the delta-delta Ct method for RT-PCR, we observed minimal expression levels of gpx2, gpx5, gpx6, and gpx7 in the brain tissue from the healthy control animals, while gpx3 and gpx8 exhibited moderate expression levels. Notably, gpx1 and gpx4 displayed the highest expression levels. Gender differences were not observed in the expression profiles of these isoforms in the control animals. Conversely, the tumor tissue exhibited elevated relative expression levels in all isoforms, except for gpx4, which remained unchanged, and gpx5, which exhibited alterations solely in female animals. Moreover, except for gpx1, which displayed no gender differences, the relative expression values of gpx2, gpx3, gpx6, gpx7, and gpx8 were significantly higher in the male animals compared to their female counterparts. Hence, the analysis of glutathione peroxidase isoforms may serve as a valuable approach for discerning the behavior of brain tumors in clinical settings.

show abstract

Section: Discussionmentioning

confidence: 99%

Glutathione Peroxidase gpx1 to gpx8 Genes Expression in Experimental Brain Tumors Reveals Gender-Dependent Patterns

Cueto-Ureña,

Ramírez-Expósito,

Mayas

et al. 2023

Genes

View full text Add to dashboard Cite

show abstract

“…Recent studies have shown that GPX8 can maintain the invasive mesenchymal-like phenotype of breast cancer cells through the IL-6/STAT3 axis [23]. GPX8 has been recognized as a prognostic marker for cancers such as gastric cancer and primary glioma [24,25]. In clear cell renal cell carcinoma, GPX8 silencing inhibits tumorigenesis by regulating nicotinamide N-methyltransferase (NNMT) [26].…”

Section: Introductionmentioning

confidence: 99%

GPX8+ cancer-associated fibroblast, as a cancer-promoting factor in lung adenocarcinoma, is related to the immunosuppressive microenvironment

Bai,

Han,

Dong

et al. 2024

BMC Med Genomics

View full text Add to dashboard Cite

Background Cancer-associated fibroblasts (CAFs) play a crucial role in the tumor microenvironment of lung adenocarcinoma (LUAD) and are often associated with poorer clinical outcomes. This study aimed to screen for CAF-specific genes that could serve as promising therapeutic targets for LUAD. Methods We established a single-cell transcriptional profile of LUAD, focusing on genetic changes in fibroblasts. Next, we identified key genes associated with fibroblasts through weighted gene co-expression network analysis (WGCNA) and univariate Cox analysis. Then, we evaluated the relationship between glutathione peroxidase 8 (GPX8) and clinical features in multiple independent LUAD cohorts. Furthermore, we analyzed immune infiltration to shed light on the relationship between GPX8 immune microenvironment remodeling. For clinical treatment, we used the tumor immune dysfunction and exclusion (TIDE) algorithm to assess the immunotherapy prediction efficiency of GPX8. After that, we screened potential therapeutic drugs for LUAD by the connectivity map (cMAP). Finally, we conducted a cell trajectory analysis of GPX8+ CAFs to show their unique function. Results Fibroblasts were found to be enriched in tumor tissues. Then we identified GPX8 as a key gene associated with CAFs through comprehensive bioinformatics analysis. Further analysis across multiple LUAD cohorts demonstrated the relationship between GPX8 and poor prognosis. Additionally, we found that GPX8 played a role in inducing the formation of an immunosuppressive microenvironment. The TIDE method indicated that patients with low GPX8 expression were more likely to be responsive to immunotherapy. Using the cMAP, we identified beta-CCP as a potential drug-related to GPX8. Finally, cell trajectory analysis provided insights into the dynamic process of GPX8+ CAFs formation. Conclusions This study elucidates the association between GPX8+ CAFs and poor prognosis, as well as the induction of immunosuppressive formation in LUAD. These findings suggest that targeting GPX8+ CAFs could potentially serve as a therapeutic strategy for the treatment of LUAD.

show abstract

“…The prevention of accidental falls are critical events that demand attention in the care of patients with long-term CKD, as neglecting them may lead to severe consequences [1][2][3] . Chronic kidney disease (CKD) is a kidney disease due to pathological changes or functional reduction of the kidney that lasts for more than 3 months 4,5 . Without timely treatment, CKD may gradually progress to end-stage kidney disease (ESKD), which is difficult to treat 6 .…”

Section: Introductionmentioning

confidence: 99%

Characteristics of Accidental Falls in the Patients with Chronic Kidney Diseases: A 14-Year Retrospective Study and Review

Liao,

Zhang,

Peng

et al. 2023

Preprint

View full text Add to dashboard Cite

BackgroundAccidental falls pose a high-risk that should not be overlooked in patients with chronic kidney disease (CKD), as they can result in significant injury or even fatality. This study aimed to investigate the characteristics of CKD patients with fall injuries during hospitalization, discuss potential mechanisms, and to provide an overview of existing prevention methods.MethodsFalls of all patients in our Nephrology ward from 2009 to 2022 were recorded and counted. 48 patients were enrolled. Patient characteristics, injury distribution, cause of fall injury, relevant blood biochemical indicators, and recovery conditions were counted.ResultsThere were 22,053 hospitalized patients during the study period, with a fall rate of approximately 0.218%. Patients are prone to involuntary falls due to muscle weakness and confusion during nighttime and early morning activities. Injuries are mainly to the head and there is a risk of serious injury and fracture. CKD is associated with anemia, hypertension, water-electrolytes imbalance and secondary hyperparathyroidism. Blood tests showed that patients commonly had anemia, malnutrition, low immunity, as well as abnormal muscle and neuromodulatory ion levels, such as low calcium, low potassium and high phosphorus. Moreover, Patients usually have low blood pressure control ability.ConclusionLong-term CKD may lead to subjective dysfunction and motor dysfunction by inducing anemia, malnutrition, water-electrolytes imbalance, and blood pressure control ability, thus making patients prone to falls. This study has important implications for hospital ward safety management and fall prevention in CKD patients.

show abstract

GPX8 as a Novel Prognostic Factor and Potential Therapeutic Target in Primary Glioma

Cited by 6 publications

References 38 publications

Glutathione Peroxidase gpx1 to gpx8 Genes Expression in Experimental Brain Tumors Reveals Gender-Dependent Patterns

Glutathione Peroxidase gpx1 to gpx8 Genes Expression in Experimental Brain Tumors Reveals Gender-Dependent Patterns

GPX8+ cancer-associated fibroblast, as a cancer-promoting factor in lung adenocarcinoma, is related to the immunosuppressive microenvironment

Characteristics of Accidental Falls in the Patients with Chronic Kidney Diseases: A 14-Year Retrospective Study and Review

Contact Info

Product

Resources

About