Gracilibacillus gen. nov., with description of Gracilibacillus halotolerans gen. nov., sp. nov.; transfer of Bacillus dipsosauri to Gracilibacillus dipsosauri comb. nov., and Bacillus salexigens to the genus Salibacillus gen. nov., as Salibacillus salexigens comb. nov.

Wainø, Michael; Tindall, Brian J.; Schümann, Peter; Ingvorsen, Kjeld

doi:10.1099/00207713-49-2-821

Cited by 242 publications

(223 citation statements)

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“…Investigation of 16S and 23S rRNA gene sequences has revealed a high heterogeneity in the genus Bacillus [7] and following extensive phylogenetic analysis, the genus has undergone major taxonomic revision leading to the establishment of new genera [8]. The genera, Geobacillus [9], Ureibacillus [10], and Aneurinibacillus [11] are outcome of this classification.…”

Section: Introductionmentioning

confidence: 99%

Identification of Thermophilic Bacterial Strains Producing Thermotolerant Hydrolytic Enzymes from Manure Compost

et al. 2011

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Ten thermophilic bacterial strains were isolated from manure compost. Phylogenetic analysis based on 16S rRNA genes and biochemical characterization allowed identification of four different species belonging to four genera: Geobacillus thermodenitrificans, Bacillus smithii, Ureibacillus suwonensis and Aneurinibacillus thermoaerophilus. PCR-RFLP profiles of the 16S-ITS-23S rRNA region allowed us to distinguish two subgroups among the G. thermodenitrificans isolates. Isolates were screened for thermotolerant hydrolytic activities (60-65°C). Thermotolerant lipolytic activities were detected for G. thermodenitrificans, A. thermoaerophilus and B. smithii. Thermotolerant protease, a-amylase and xylanase activities were also observed in the G. thermodenitrificans group. These species represent a source of potential novel thermostable enzymes for industrial applications.

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Section: Introductionmentioning

confidence: 99%

Identification of Thermophilic Bacterial Strains Producing Thermotolerant Hydrolytic Enzymes from Manure Compost

et al. 2011

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“…Cells are resistant to gentamicin (10 mg), kanamycin (30 mg), nalidixic acid (20 mg) and streptomycin (10 mg), Table 1. Differential characteristics between strain YIM kkny16 T and recognized species of the genus Virgibacillus Taxa: 1, strain YIM kkny16 T (data from the present study); 2, V. marismortui (Arahal et al, 1999(Arahal et al, , 2000Heyrman et al, 2003); 3, V. halodenitrificans (Yoon et al, 2004); 4, V. olivae (Quesada et al, 2007); 5, V. carmonensis (Heyrman et al, 2003); 6, V. proomii (Heyndrickx et al, 1999;Heyrman et al, 2003); 7, V. pantothenticus (Heyndrickx et al, 1999;Heyrman et al, 2003); 8, V. dokdonensis (Yoon et al, 2005); 9, V. necropolis (Heyrman et al, 2003); 10, V. salexigens (Garabito et al, 1997;Wainø et al, 1999;Heyrman et al, 2003); 11, V. koreensis (Lee et al, 2006); 12, V. halophilus . +, Positive; 2, negative; V, variable; W, weakly positive; pr, predominant amount; tr, trace; ND, no data available.…”

Section: Description Of Virgibacillus Kekensis Sp Novmentioning

confidence: 99%

Virgibacillus kekensis sp. nov., a moderately halophilic bacterium isolated from a salt lake in China

Chen

Cui

Fritze

et al. 2008

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLO

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A Gram-positive, moderately halophilic, motile, strictly aerobic, endospore-forming, oxidase-and catalase-positive, rod-shaped bacterium, strain YIM kkny16 T , was isolated from a saline mud sample collected from the Keke salt lake in the Qaidam Basin, north-west China. This isolate grew in the presence of 0-25 % (w/v) NaCl and at pH 6.0-10.0 and 10-50 6C; optimum growth was observed with 10 % (w/v) NaCl and at pH 7.0 and 37 6C. Strain YIM kkny16 T had meso-diaminopimelic acid as the diagnostic diamino acid, MK-7 as the predominant respiratory quinone, with a significant amount of MK-6, and anteiso-C 15 : 0 , iso-C 14 : 0 and C 16 : 1 v7c alcohol as major fatty acids. Major polar lipids were diphosphatidylglycerol and phosphatidylglycerol. The DNA G+C content was 41.8 mol%. Phylogenetic analysis based on 16S rRNA gene sequences confirmed that strain YIM kkny16 T was a member of the genus Virgibacillus, exhibiting sequence similarities of 94.9-97.3 % to the type strains of recognized Virgibacillus species. Strain YIM kkny16 T could be differentiated from recognized Virgibacillus species based on phenotypic characteristics, chemotaxonomic differences, phylogenetic analysis and DNA-DNA hybridization data. On the basis of evidence from this polyphasic study, strain YIM kkny16 T is considered to represent a novel species of the genus Virgibacillus, for which the name Virgibacillus kekensis sp. nov. is proposed. The type strain is YIM kkny16 T (5DSM 17056 T 5CGMCC 1.6298 T ).At the time of writing, the genus Virgibacillus comprises 11 recognized species, with Virgibacillus olivae (Quesada et al., 2007) and Virgibacillus halophilus (An et al., 2007) being the most recently described. Virgibacillus pantothenticus (Heyndrickx et al., 1998) is the type species. During a recent study of the microbial diversity of the Keke salt lake in the Qaidam Basin of Qinghai Province, north-west China, a moderately halophilic rod-shaped bacterium, designated strain YIM kkny16 T , was isolated from a saline mud sample. Phenotypic and chemotaxonomic characterization, together with phylogenetic analysis based on 16S rRNA gene sequences, showed that strain YIM kkny16 T was related to members of the genus Virgibacillus, but represented a novel species.Strain YIM kkny16 T was isolated from a saline mud sample by plating 1 : 10 serial dilutions of the sample on Difco marine agar 2216 (MA; pH 7.2) at 28 u C for 2 weeks. The strain was maintained both in Difco marine broth (MB) supplemented with 20 % (v/v) glycerol at 280 u C and as lyophilized cultures at 4 u C. Unless otherwise indicated, morphological and physiological studies were performed with cells grown on MA supplemented with 10 % (w/v) NaCl, and on ISP medium 2 agar (Shirling & Gottlieb, 1966), nutrient agar and trypticase soy agar (BBL) as controls. Cells for chemotaxonomic and molecular systematic studies were grown in MB supplemented with 10 % (w/v) NaCl in shake flasks (with shaking at aboutThe GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain YIM kkny1...

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“…The genus Virgibacillus was created by the reclassification of Bacillus pantothenticus (Heyndrickx et al, 1998;Wainø et al, 1999) as Virgibacillus pantothenticus, and the genus description was subsequently emended by Heyrman et al (2003). At the time of writing, the genus comprised 26 species with validly published names, including the recently described Virgibacillus soli (Kämpfer et al, 2011), Virgibacillus alimentarius (Kim et al, 2011), Virgibacillus campisalis , Virgibacillus halotolerans (Seiler and Wenning., 2013) and Virgibacillus oceani (Yin et al, 2015).…”

mentioning

confidence: 99%

Virgibacillus flavescens sp. nov., isolated from marine sediment

Zhang

Schümann

et al. 2016

International Journal of Systematic and Evolutionary Microbiology

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A Gram-stain-variable, rod-shaped, motile bacterial strain, designated S1-20 T , was isolated from marine sediment of the Yellow Sea in China. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain S1-20 T was related to the genus Virgibacillus and had highest 16S rRNA gene sequence similarities with the type strains of Virgibacillus necropolis (98.1 %), Virgibacillus arcticus (97.7 %) and Virgibacillus carmonensis (97.3 %). The predominant cellular fatty acids of strain S1-20 T were anteiso-C 15 : 0 and anteiso-C 17 : 0 . The main menaquinone was MK-7. The genomic DNA G+C content of strain S1-20 T was 38.3 mol%. The polar lipids profile contained diphosphatidylglycerol, phosphatidylglycerol, five unidentified phospholipids, one unknown aminophospholipid and an aminolipid. Combined data from phenotypic, phylogenetic and DNA-DNA relatedness studies demonstrated that strain S1-20 T is a representative of a novel species of the genus Virgibacillus, for which the name Virgibacillus flavescens sp. nov. is proposed. The type strain is S1-20 T (5LMG 28381 T 5DSM 29015 T ).

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Gracilibacillus gen. nov., with description of Gracilibacillus halotolerans gen. nov., sp. nov.; transfer of Bacillus dipsosauri to Gracilibacillus dipsosauri comb. nov., and Bacillus salexigens to the genus Salibacillus gen. nov., as Salibacillus salexigens comb. nov.

Cited by 242 publications

References 28 publications

Identification of Thermophilic Bacterial Strains Producing Thermotolerant Hydrolytic Enzymes from Manure Compost

Identification of Thermophilic Bacterial Strains Producing Thermotolerant Hydrolytic Enzymes from Manure Compost

Virgibacillus kekensis sp. nov., a moderately halophilic bacterium isolated from a salt lake in China

Virgibacillus flavescens sp. nov., isolated from marine sediment

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