2016
DOI: 10.1073/pnas.1609981113
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Grad-seq guides the discovery of ProQ as a major small RNA-binding protein

Abstract: The functional annotation of transcriptomes and identification of noncoding RNA (ncRNA) classes has been greatly facilitated by the advent of next-generation RNA sequencing which, by reading the nucleotide order of transcripts, theoretically allows the rapid profiling of all transcripts in a cell. However, primary sequence per se is a poor predictor of function, as ncRNAs dramatically vary in length and structure and often lack identifiable motifs. Therefore, to visualize an informative RNA landscape of organi… Show more

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Cited by 272 publications
(516 citation statements)
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“…To date, a small number of proteins have been identified that specifically bind to sRNA molecules in numerous bacterial species, including Hfq, CsrA, and more recently FinO-like proteins (Romeo et al 2013;Wagner and Romby 2015;Attaiech et al 2016;Smirnov et al 2016Smirnov et al , 2017. The protein ProQ, a FinO family member, was originally identified as an osmoregulatory factor required for optimal expression of the proline channel protein ProP (Kunte et al 1999;Chaulk et al 2011;Kerr et al 2014).…”
Section: Introductionmentioning
confidence: 99%
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“…To date, a small number of proteins have been identified that specifically bind to sRNA molecules in numerous bacterial species, including Hfq, CsrA, and more recently FinO-like proteins (Romeo et al 2013;Wagner and Romby 2015;Attaiech et al 2016;Smirnov et al 2016Smirnov et al , 2017. The protein ProQ, a FinO family member, was originally identified as an osmoregulatory factor required for optimal expression of the proline channel protein ProP (Kunte et al 1999;Chaulk et al 2011;Kerr et al 2014).…”
Section: Introductionmentioning
confidence: 99%
“…The protein ProQ, a FinO family member, was originally identified as an osmoregulatory factor required for optimal expression of the proline channel protein ProP (Kunte et al 1999;Chaulk et al 2011;Kerr et al 2014). However, ProQ has now emerged to function more broadly as a global sRNA binding protein in Salmonella, and a similar role is predicted for proteins containing ProQ/FinO domains found in divergent bacterial species (Smirnov et al 2016). In Legionella pneumophila, a ProQ/FinO domain protein has been shown to be required for regulation of an sRNA controlling natural competence (Attaiech et al 2016).…”
Section: Introductionmentioning
confidence: 99%
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“…Recently, novel RNA-binding proteins such as CsrA and ProQ have also been proposed to function as sRNAs chaperones in bacteria [21].…”
Section: Introductionmentioning
confidence: 99%