Graded effects of dry-feed added sodium benzoate/ascorbic acid combination on neurobehaviour, brain markers of oxidative stress, and markers of inflammation in mice

Olofinnade, Anthony Tope; Onaolapo, Adejoke Yetunde; Onaolapo, Olakunle James

doi:10.21203/rs.3.rs-2480579/v1

Cited by 2 publications

(1 citation statement)

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“…Spontaneous alternation behaviour assesses the propensity of rodents to alternate conventionally non-reinforced choices of the Y or radial arm maze on successive chances. The sequence of arm entries is assessed and scored as previously described [41,42,45].…”

Section: Memory Tests (Y-and Radial Arm-maze)mentioning

confidence: 99%

Dietary zinc protects against methotrexate-induced neurotoxicity in rats via modulation of oxidative stress, inflammation and neuronal energy metabolism

Onaolapo

Okunola

Onaolapo

2023

Preprint

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Background: Increasing incidence of cancers and cancer chemotherapy-induced neurotoxicities makes it imperative to research compounds with neuroprotective potential that can do not impede therapy. Objective: To examine the effect of dietary zinc supplementation on methotrexate-induced changes in neurobehaviour and neurochemistry and hippocampal morphology in rats. Methods: Adult male rats were assigned into five groups of twelve animals each. Group were normal control and methotrexate control fed standard rodent chow and three groups of rats fed zinc supplemented diet at 25, 50 and 100 mg/kg of feed respectively. Standard and zinc supplemented diet were administered daily for 21 days. Animals in the normal control were administered intraperitoneal injection (i.p) of normal saline at 2ml/kg, while those in the methotrexate and zinc groups were administered i.p methotrexate at 20 mg/kg/day on days 19-21. On day 22, animals were exposed to behavioural paradigm (open field, Y-maze, radial arm maze, elevated plus maze and behavioural despair test). After the last behavioural test, animals were sacrificed and blood taken for the assessment of Tumour necrosis factor-α, interleukin 1β and interleukin 10), malondialdehyde levels and total antioxidant capacity. The hippocampus was either homogenised for the assessment of dopamine, serotonin, acetylcholine and brain derived neurotropic factor levels or processed for histological study. Results: Dietary zinc protected against methotrexate-induced changes in weight, food intake, cognition, hippocampal histomorphology and neuron specific enolase immunohistochemistry. Conclusion: Dietary zinc supplementation protects against methotrexate induced neurotoxicities by modulating oxidative stress, inflammatory markers brain neurotransmitters and metabolism in rats.

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Section: Memory Tests (Y-and Radial Arm-maze)mentioning

confidence: 99%

Dietary zinc protects against methotrexate-induced neurotoxicity in rats via modulation of oxidative stress, inflammation and neuronal energy metabolism

Onaolapo

Okunola

Onaolapo

2023

Preprint

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Induced-hyperandrogenism in female rats: assessing possible modulation of neurobehavioural and neurotransmitter changes following clomiphene/letrozole intervention

Aworinde

Olofinnade

Onaolapo

2023

Preprint

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Hyperandrogenism is the excessive production of androgenic hormones resulting in infertility in a number of women. While letrozole and clomiphene citrate have been used to increase chances of achieving pregnancy, their effects on the brain has been scarcely studied. This study examined the effects of clomiphene and letrozole alone or in combination on neurobehavioural and neurochemical changes in female rats exposed to testosterone. Weaned rats were assigned into eight groups of ten each. Animals were grouped as normal control administered vehicle (normal saline) orally at 10 ml/kg or subcutaneously at 2 ml/kg, three groups administered clomiphene (CLOM) at 100 microgramme/kg, letrozole (LETR) at 5 mg/kg and or a combination of clomiphene and letrozole (CLOM/LETR) orally and saline subcutaneously. There were also four groups Testosterone (Test), Test/CLOM, Test/LETR or Test/CLOM+LETR administered testosterone enantate subcutaneously at 1 mg/100 g. Testosterone or saline was administered from day 1-35, while beginning on day 36, clomiphene, letrozole or saline was administered daily for 10 days. At the end of the dosing period, animals were exposed to different behavioural paradigms. After the behavioural tests, animals were sacrificed, the cerebral cortex was homogenised for the assessment of biochemical assays. The result showed an increase in body weight, food intake, locomotor activity, rearing and self grooming with CLOM, LETR and CLOM/LETR in all treated groups. Decreased spatial working memory and anxiolysis was observed with letrozole and/or clomiphene. Increased oxidative stress, decreased total antioxidant capacity, altered inflammatory cytokines and brain neurotransmitter were observed with letrozole and /or clomiphene. In conclusion, the administration of clomiphene and/or letrozole was associated with significant alterations in brain function, oxidative stress, inflammatory markers and brain neurotransmitter levels.

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Graded effects of dry-feed added sodium benzoate/ascorbic acid combination on neurobehaviour, brain markers of oxidative stress, and markers of inflammation in mice

Cited by 2 publications

References 44 publications

Dietary zinc protects against methotrexate-induced neurotoxicity in rats via modulation of oxidative stress, inflammation and neuronal energy metabolism

Dietary zinc protects against methotrexate-induced neurotoxicity in rats via modulation of oxidative stress, inflammation and neuronal energy metabolism

Induced-hyperandrogenism in female rats: assessing possible modulation of neurobehavioural and neurotransmitter changes following clomiphene/letrozole intervention

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