Gradient for<scp>d</scp>‐glucose and linoleic acid uptake along the crypt‐villus axis of rabbit jejunal brush border membrane vesicles

Fingerote, R. J.; Doring, K.; Thomson, A. B. R.

doi:10.1007/bf02537151

Cited by 17 publications

(9 citation statements)

References 59 publications

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“…To address this question, we used an experimental approach aimed at isolating in parallel the villus tip and crypt cells from the rabbit small intestine. The present results indicate that both the activity and expression of SGLT1 were much higher in villi than in crypt cell BBM, confirming this symporter to reflect the degree of enterocyte development along the small intestinal crypt-villus axis [29][30][31][32][33]. On the other hand, Cl -/H + symport activity appeared to be unchanged along the crypt-villus axis (Fig.…”

Section: Discussionsupporting

confidence: 60%

“…As expected in these conditions [29][30][31][32][33], D-glucose uptake by both villus and crypt cell BBM exhibited the characteristic overshoot phenomenon as demonstrated by the 60 s / equilibrium D-glucose concentration ratios, r60, that are greater than unity (Fig. 1).…”

Section: Resultsmentioning

confidence: 74%

“…On the other hand, Na + -D-glucose symport activity has been demonstrated to occur in both villus and crypt cell BBM of rabbit and rat [29][30][31][32][33]. SGLT1 activity strongly diminished from the villus to the crypt region, as did sucrase activity, reflecting the degree of enterocyte development [29,32,33].…”

Section: Resultsmentioning

confidence: 99%

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Mechanisms of Net Chloride Secretion During Rotavirus Diarrhea in Young Rabbits : Do Intestinal Villi Secrete Chloride?

Lorrot

Benhamadouche-Casari²,

Vasseur³

2006

Cell Physiol Biochem

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Rotaviral diarrheal illness is one of the most common infectious diseases in children worldwide, but our understanding of its pathophysiology is limited. This study examines whether the enhanced net chloride secretion during rotavirus infection in young rabbits may occur as a result of hypersecretion in crypt cells that would exceed the substantial Cl^- reabsorption observed in villi. By using a rapid filtration technique, we evaluated transport of ³⁶Cl and D-¹⁴C glucose across brush border membrane (BBM) vesicles purified from villus tip and crypt cells isolated in parallel from the entire small intestine. Rotavirus infection impaired SGLT1-mediated Na⁺-D-glucose symport activity in both villus and crypt cell BBM, hence contributing to the massive water loss along the cryptvillus axis. In the same BBM preparations, rotavirus failed to stimulate the Cl^- transport activities (Cl^-/H⁺ symport, Cl^-/anion exchange and voltage-activated Cl^- conductance) at the crypt level, but not at the villus level, questioning, therefore, the origin of net chloride secretion. We propose that the chloride carrier might function in both normal (absorption) and reversed (secretion) modes in villi, depending on the direction of the chloride electrochemical gradient resulting from rotavirus infection, agreeing with our results that rotavirus accelerated both Cl^- influx and Cl^- efflux rates across villi BBM.

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Section: Discussionsupporting

confidence: 60%

Section: Resultsmentioning

confidence: 74%

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Mechanisms of Net Chloride Secretion During Rotavirus Diarrhea in Young Rabbits : Do Intestinal Villi Secrete Chloride?

Lorrot

Benhamadouche-Casari²,

Vasseur³

2006

Cell Physiol Biochem

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“…The maximal activities of disaccharidases are localized near the top of intestinal villi, with minimal values near the crypts [25]. A similar distribution along the crypt-apical axis has been observed for glucose transporters SGLT1 [26,27].…”

Section: Mathematical Modelingsupporting

confidence: 67%

Kinetics and mechanisms of glucose absorption in the rat small intestine under physiological conditions

Груздков¹,

Громова²,

Grefner³

et al. 2012

JBPC

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Previous studies have shown two components of glucose absorption in the small intestine: a secondary active transport through SGLT1, and unsaturated component, recently attributed mainly to the facilitated diffusion through GLUT2, but the relationship between these two components under physiological conditions remains controversial. In chronic experiments on nonanesthetized rats we investigated for the first time the kinetics of maltose hydrolysis and glucose absorption in the isolated loop of the small intestine in a wide range of maltose and glucose concentrations (25 -200 mmol/l glucose). The processes were simulated on mathematical models which took into account the current views about mechanisms of hydrolysis and transport of nutrients and geometric characteristics of the intestinal surface. The results of chronic experiments and mathematical simulation have shown that under the close to physiological conditions the glucose transport mediated by SGLT1 is the main mechanism of its absorption in comparison with the unsaturated component. This was demonstrated not only at low, but also at high substrate concentrations. We conclude that correct evaluation of the relative contribution of different mechanisms in glucose transport through the intestinal epithelium requires taking into account the geometric specificities of its surface.

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“…As variance increased with the size of the y-axis variable (rate of uptake of glucose), data points were weighted in proportion to the reciprocal of the within-concentration estimates of variance (14).…”

Section: Methodsmentioning

confidence: 99%

Variablity of the intestinal uptake of lipids is genetically determined in mice

Keelan

Hui

Wild

et al. 2000

Lipids

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The response of the plasma cholesterol concentration to changes in dietary lipids varies widely in humans and animals. There are variations in the in vivo absorption of cholesterol between different strains of mice. This study was undertaken in three strains of inbred mice to test the hypotheses that: (i) there are strain differences in the in vitro uptake of fatty acids and cholesterol and (ii) the adaptability of the intestine to respond to variations in dietary lipids is genetically determined. An in vitro intestinal ring technique was used to assess the uptake of medium- and long-chain fatty acids and cholesterol into jejunum and ileum of adult DBA/2, C57BL6, and C57L/J mice. The jejunal uptake of cholesterol was similar in C57L/J, DBA/2, or C57BL6 fed ad libitum a low-fat (5.7% fat, no cholesterol) chow diet. This is in contrast to a previous demonstration that in vivo cholesterol absorption was lower in C57L/J than in the other murine strains. The jejunal uptake of several long-chain fatty acids was greater in DBA/2 fed for 4 wk the high-fat (15.8% fat and 1.25% cholesterol) as compared with the low-fat diet. Furthermore, on the high-fat diet, the uptake of many long-chain fatty acids was higher in DBA/2 than in C57BL6 or C57L/J. The differences in cholesterol and fatty acid uptake were not explained by variations in food uptake, body weight gain, or the weight of the intestine. In summary: (i) there are strain differences in the in vitro intestinal uptake of fatty acids but not of cholesterol; (ii) a high-fat diet enhances the uptake of long-chain fatty acids in only one of the three strains examined in this study; and (iii) the pattern of strain- and diet-associated alterations in the in vivo absorption of cholesterol differs from the pattern of changes observed in vitro. We speculate that genetic differences in cholesterol and fatty acid uptake are explained by variations in the expression of protein-mediated components of lipid uptake.

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Gradient ford‐glucose and linoleic acid uptake along the crypt‐villus axis of rabbit jejunal brush border membrane vesicles

Cited by 17 publications

References 59 publications

Mechanisms of Net Chloride Secretion During Rotavirus Diarrhea in Young Rabbits : Do Intestinal Villi Secrete Chloride?

Mechanisms of Net Chloride Secretion During Rotavirus Diarrhea in Young Rabbits : Do Intestinal Villi Secrete Chloride?

Kinetics and mechanisms of glucose absorption in the rat small intestine under physiological conditions

Variablity of the intestinal uptake of lipids is genetically determined in mice

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