Gradient of RNA distribution in the cytoplasm of Acetabularia mediterranea

Naumova, L.P.; Pressman, Etan; Sandakchiev, Lev S.

doi:10.1016/0304-4211(76)90052-3

Cited by 7 publications

(5 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our results demonstrate the presence of RNA throughout the entire cell length, and identified the highest amount of mRNA at the apical end of the cell (the cap) decreasing towards the basal end (the rhizoid), confirming earlier discoveries of an apical-basal gradient of RNA in A. acetabulum (Baltus et al, 1968; Hämmerling, 1936; Werz, 1955). However, while it has been believed that this gradient is due to different concentrations of RNA encoded by the chloroplasts, and not nuclear encoded RNAs (Naumova et al, 1976), we rather find that the apical-basal gradient is mainly caused by nuclear encoded mRNAs.…”

Section: Discussioncontrasting

confidence: 89%

“…He also showed that these substances were distributed in gradients, with the highest accumulation at the cap and rhizoid regions (Hämmerling, 1934c; Hämmerling, 1963). These morphogenetic substances were later shown to consist of mRNA (Baltus et al, 1968; Garcia et al, 1986; Kloppstech, 1977; Naumova et al, 1976), but the content of the RNA gradient, and whether it was composed of homogeneously or differentially distributed RNA transcripts was not known.…”

Section: Introductionmentioning

confidence: 99%

“…A few studies have tried to decipher the RNA gradient in A. acetabulum . Naumova et al (1976) argued that the gradient was due to differential metabolism of chloroplast ribosomal RNA along the cell rather than differential transportation of nuclear mRNA. A more recent study by Vogel et al (2002) examined the expression of 13 house-keeping genes and found differential accumulation of several gene transcripts, where some transcripts seemed to accumulate in the rhizoid, some in the cap, while others were evenly distributed along the cellular body.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Compartmentalization of mRNAs in the giant, unicellular green algaeAcetabularia acetabulum

Andresen

Orr

Shalchian-Tabrizi

et al. 2020

Preprint

View full text Add to dashboard Cite

Acetabularia acetabulum is a single-celled green alga previously used as a model species for studying the role of the nucleus in cell development and morphogenesis. The highly elongated cell, which stretches several centimeters, harbors a single nucleus located in the basal end. Although A. acetabulum historically has been an important model in cell biology, almost nothing is known about its gene content, or how gene products are distributed in the cell. To study the composition and distribution of mRNAs in A. acetabulum, we have used quantitative RNA-seq to sequence the mRNA content of four sections of adult A. acetabulum cells. We found that although mRNAs are present throughout the cell, there are large pools of distinct mRNAs localized to the different subcellular sections. Conversely, we also find that gene transcripts related to intracellular transport are evenly distributed throughout the cell. This distribution hints at post-transcriptional regulation and selective transport of mRNAs as mechanisms to achieve mRNA localization in A. acetabulum.

show abstract

Section: Discussioncontrasting

confidence: 89%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Compartmentalization of mRNAs in the giant, unicellular green algaeAcetabularia acetabulum

Andresen

Orr

Shalchian-Tabrizi

et al. 2020

Preprint

View full text Add to dashboard Cite

show abstract

“…In terms of the framework outlined in Table 1, it appears that the localization of phosphorylase activity is either the result of more template-specific RNA in the apical region (Level I) or a greater rate of translation in the apical region of equally distributed RNA template fo r phosphorylase (Level II). Although an apico-basal distribution of RNA has been reported fo r Acetabu Iorio (55,112), this most probably represents nontemplate RNA and differences in turnover rates. Before mechanisms are sought for the transport of template RNA to the apical region or the translation of cap-specific template RNA in that region, more direct evidence should be obtained to fu rther substantiate the interesting results reported on the intracellular distribution of this enzyme.…”

Section: Polarity Expressionmentioning

confidence: 94%

Development of Cell Polarity

Quatrano¹

1978

Annu. Rev. Plant. Physiol.

134

View full text Add to dashboard Cite

“…There is independent biochemical evidence for apical-basal gradients of protein synthesis (30, 66,89,90), of ribosomal and mRNA (30, 40, 46, 47, 56-58, 65,84,97), of thiol groups (110), and even of specific enzymatic activities (e.g. 116) in this alga.…”

Section: The Case For Translational Control Of Morphogenesis During Dmentioning

confidence: 97%

ELABORATION OF BODY PLAN AND PHASE CHANGE DURING DEVELOPMENT OF ACETABULARIA: How Is the Complex Architecture of a Giant Unicell Built?

Mandoli

1998

Annu. Rev. Plant. Physiol. Plant. Mol. Biol.

View full text Add to dashboard Cite

While uninucleate and unicellular, Acetabularia acetabulum establishes and maintains functionally and morphologically distinct body regions and executes phase changes like those in vascular plants. Centimeters tall at maturity, this species has allowed unusual experimental approaches. Amputations revealed fates of nucleate and enucleate portions from both wild type and mutants. Historically, graft chimeras between nucleate and enucleate portions suggested that morphological instructions were supplied by the nucleus but resided in the cytoplasm and could be expressed interspecifically. Recently, graft chimeras enabled rescue of mutants arrested in vegetative phase. Since the 1930s, when Acetabularia provided the first evidence for the existence of mRNAs, a dogma has arisen that it uses long-lived mRNAs to effect morphogenesis. While the evidence favors translational control, the postulated mRNAs have not been identified, and the mechanism of morphogenesis remains unknown. Amenable to biochemistry, physiology, and both classical and molecular genetics, Acetabularia may contribute yet new insights into plant development and morphogenesis.

show abstract

Gradient of RNA distribution in the cytoplasm of Acetabularia mediterranea

Cited by 7 publications

References 7 publications

Compartmentalization of mRNAs in the giant, unicellular green algaeAcetabularia acetabulum

Compartmentalization of mRNAs in the giant, unicellular green algaeAcetabularia acetabulum

Development of Cell Polarity

ELABORATION OF BODY PLAN AND PHASE CHANGE DURING DEVELOPMENT OF ACETABULARIA: How Is the Complex Architecture of a Giant Unicell Built?

Contact Info

Product

Resources

About