Gradient RP-HPLC method development and validation for simultaneous estimation of paclitaxel and albendazole

Abstract

The goal of this research is to provide an accurate and exact technique for estimating Albendazole and Paclitaxel simultaneously utilizing gradient RP-HPLC (high performance liquid chromatography). A UV detector fixed at 230 nm was used to estimate albendazole and paclitaxel simultaneously. As the solvent system, acetonitrile and water in the ratio of 50:50 was used in the presence of 0.1 % trifluoroacetic acid. Mobile phase was run on a Inertsil ODS 3V 150 4.6 mm or equivalent column in Gradient mode at a flow rate of 1.0 mL/min. By this method albendazole and paclitaxel showed 6.199 and 9.684 retention time (Rt) respectively with continuous run upto 25 min. In the prescribed concentration range, the calibration curves for each analyte were determined to be linear (r2 > 0.994). The percentage of recovery was found to be within the limit at each level (98.0 %to 102.0 %). All validation parameters were determined to be acceptable, including system precision, method precision, linearity, range, accuracy, ruggedness, robustness, solution, and mobile phase stability findings. This suggests that the HPLC simultaneous approach for determining albendazole and paclitaxel assays is accurate. S

QBD approach to develop stability indicating RP-HPLC method development for Levosulpiride and Ilaprazole

“…Literature survey revealed that UV, HPLC and HPTLC methods are reported for the estimation of Ilaprazole and levosulpiride either in alone or in combination with other drugs. However no method has been reported for simultaneous estimation of these drugs in combination using QbD based 3 3…”