Gradient thin-layer chromatography of coumarins and furocoumarins

Głowniak, Kazimierz; Matysik, G.; Bieganowska, M. L.; Soczewiński, Edward

doi:10.1007/bf02268780

Cited by 19 publications

(6 citation statements)

References 10 publications

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“…Analysis of these compounds in complex matrices such as crude plant extracts is, therefore, of special interest. Analytical methods described in the literature rely on TLC [3], GC [4] and HPLC [5] and on HPLC-MS coupling techniques using the moving-belt [6] and particlebeam interfaces [7]. This paper presents an improved HPLC method and an MECC method for the separation of coumarins.…”

Section: Introductionmentioning

confidence: 99%

HPLC-MS and MECC analysis of coumarins

1997

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SummaryMECC separation of 11 coumarins has been achieved by use of a running electrolyte at pH 10.4 prepared from 50 mM boric acid, 10 mM sodium tetraborate and 100 mM sodium hydroxide. The buffer solution contained 50 mM SDS and, as organic modifier, 1% n-propanol. The applied voltage was 25 kV and the temperature of the capillary was kept constant at 20 ~ HPLC baseline separation of the coumarin mixture was obtained by use of a reversed-phase column and an acetonitrile-water solvent gradient. UV detection was performed at 205 nm. Peak assignment and purity control were achieved by HPLC-mass spectrometry with either an electrospray interface or an atmospheric-pressure chemical-ionization interface. Compounds were detected in either negative-or positive-ion modes. These MECC and HPLC-MS methods are suitable for 'fingerprint' analysis of a number of coumarin-containing plants, e.g. Fr. Ammi visnagae, Rd. Scopoliae and Rd. Imperatoriae.

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Section: Introductionmentioning

confidence: 99%

HPLC-MS and MECC analysis of coumarins

1997

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“…Bergapten is phototoxic – , mutagenic , , and tumorigenic . For these reasons, different methodologies have been proposed for also determining bergapten contents in biological fluids ,– . Recently , , bergapten has been reported to be the component responsible for serious problems caused in humans by regular consumption of Earl Grey tea, an alimentary preparation containing bergamot extracts.…”

Section: Introductionmentioning

confidence: 99%

Comparison of the Volatile Constituents in Cold-Pressed Bergamot Oil and a Volatile Oil Isolated by Vacuum Distillation

Belsito

Carbone

Gioia

et al. 2007

J. Agric. Food Chem.

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The vacuum distillation of bergamot peels furnishes a high-quality essential oil that is totally bergapten-free. This oil was compared with that produced by distillation of cold-pressed oils and those commercially available. The oil obtained by vacuum distillation of the bergamot vegetable matrix shows a composition quite similar to that of the cold-pressed oil. It also displays qualitative characteristics that are superior with respect to those normally observed for essential oils isolated by distillation of cold-pressed oils. Oils isolated by the method presented here can constitute ideal candidates in producing foods, for example, Earl Grey tea, and cosmetic preparations.

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“…Furocoumarin separation by HPTLC was reported both on a normal or reversed phase, in one‐ or two‐dimensions, but recent methods were preferentially developed in a normal phase . Table shows an overview of recently published methods used to analyse or isolate furocoumarins by HPTLC.…”

Section: Introductionmentioning

confidence: 99%

“…21 The method was shown to be linear between 10 and 250 mg kg −1 with a LOD and an LOQ of 2.5 and 10 mg kg −1 , respectively. The method was only validated in a standardized essential oils matrix because of the lack of specificity of the UV Furocoumarin separation by HPTLC was reported both on a normal or reversed phase, in one- [24][25][26][27][28][29][30][31][32] or two-dimensions, [33][34][35][36][37] but recent methods were preferentially developed in a normal phase. 27,30,38 45 in the fruits of H. candicans.…”

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confidence: 99%

HPTLC methods for qualitative and quantitative analysis of selected furocoumarins in essential oils

Borgne¹,

Cicchetti²,

Bertrand³

2017

Flavour & Fragrance J

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The concentration of furocoumarins, known to induce mutagenesis, carcinogenesis and photodermatitis under UV radiation, is limited by the EU cosmetic regulation in consumer products such as cosmetics, sun protection and bronzing formulations. The bergapten concentration is also limited by the International Fragrance Association. Analytical methods have been proposed for their quantification using high‐performance liquid chromatography (HPLC) coupled with UV detection or mass spectrometry with limits of detection (LOD) and quantification (LOQ) in accordance with the regulation. These methods are time and solvent consuming for routine quality control analyses. In this context, simple and fast HPLC methods in one‐ (1‐D) and two‐ (2‐D) dimensions were developed for the simultaneous analysis of the 15 most common furocoumarins reported in Citrus essential oils: psoralen, bergapten, xanthotoxin, isopimpinellin, oxypeucedanin, oxypeucedanin hydrate, byakangelicol, byakangelicin, heraclenin, 8‐geranyloxypsoralen, bergamottin, imperatorin, isoimperatorin, phellopterin, epoxybergamottin along with five other furocoumarins known to be present in Angelica, bergamot or Heracleum species: angelicin, bergaptol, isobergapten, psora‐4 and 5‐(4‐phenoxybutoxy)psoralen. The 1‐D method was validated for the quantification of bergapten using a Camag TLC Scanner as a detector. The LOD was determined at 2 mg/kg. The method was linear from 2 to 100 mg/kg and the method range was defined using accuracy profiles at with an 80% tolerance interval, with a 30% error, to be 5–100 mg/kg in acetonitrile and 13–100 mg/kg in matrix.

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Gradient thin-layer chromatography of coumarins and furocoumarins

Cited by 19 publications

References 10 publications

HPLC-MS and MECC analysis of coumarins

HPLC-MS and MECC analysis of coumarins

Comparison of the Volatile Constituents in Cold-Pressed Bergamot Oil and a Volatile Oil Isolated by Vacuum Distillation

HPTLC methods for qualitative and quantitative analysis of selected furocoumarins in essential oils

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