Gradients in intact polar diacylglycerolipids across the Mediterranean Sea are related to phosphate availability

Popendorf, Kimberly J.; Tanaka, Tsuneo; Pujo‐Pay, Mireille; Lagaria, Anna; Courties, Claude; Conan, Pascal; Oriol, L.; Sofen, L. E.; Moutin, Thierry; Mooy, Benjamin A. S. Van

doi:10.5194/bg-8-3733-2011

Cited by 37 publications

(44 citation statements)

References 42 publications

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“…This conclusion is consistent with the interpretation of increased BL:PC ratios following N addition to oligotrophic seawater microcosms (Popendorf et al, 2011b), where nutrient additions were higher than the current study by one to two orders of magnitude and yielded in only small changes in community structure. In all treatment mesocosms (n = 6), phosphate concentrations dropped to below 8 nM,τ PO4 decreased to <6 min., and the balance of P uptake shifted toward larger organisms by day 6 (Figure 9); however, no significant increases in PC were observed in the A treatment.…”

Section: Ipl Signatures Of Nutrient Turnoversupporting

confidence: 80%

“…While no GSL has been reported by previous IPL surveys of surface seawater (Van Mooy and Fredricks, 2010;Popendorf et al, 2011b;Brandsma et al, 2012;Kharbush et al, 2016), GSLs have been associated with anaerobic production in subsurface FIGURE 9 | Correlated changes in IPL relative abundance with τ PO4 and fractional phosphate uptake by plankton >2 µm in control mesocosms (circles) and those amended with Saharan dust (squares) or mixed aerosol (triangles) and collected on day −1 (light gray), day 3 (dark gray), or day 6 (black).…”

Section: Hierarchical Clustering Analysis Of Ipl Alkyl Chainsmentioning

confidence: 76%

“…Section IPL Concentrations; Figure 6) may be similarly explained. Further indication of surplus P in the SD treatment stems from the ratio of BL to PC, for which increased values have been interpreted as a signal of P-limitation Popendorf et al, 2011b). Conversely, the significantly lower BL:PC value in SD mesocosms on day 6 (2.0 ± 0.3) suggest that P was in excess, relative to control (5.2 ± 1.7) and A (3.7 ± 0.7) mesocosms as well as day −1 of the SD treatment (3.8 ± 0.6; p < 0.04).…”

Section: Ipl Signatures Of Nutrient Turnovermentioning

confidence: 99%

“…In addition to these bulk biogeochemical measures of food web dynamics, the diversity of algal and bacterial cell membrane lipids provides a moderate level of taxonomic and physiological information (e.g., Sato, 1992;Guschina and Harwood, 2006;Sohlenkamp and Geiger, 2015) that can be applied to assess organic matter reactivity (Harvey and Macko, 1997;Wakeham et al, 1997), plankton communities in the ocean (e.g., Van Mooy and Fredricks, 2010), and carbon and nutrient fluxes in marine food webs (Dalsgaard et al, 2003;Sebastián et al, 2016). For example, phytoplankton populations are able to reduce their cellular P quota by substituting phospholipids with sulfolipids or betaine lipids when P is limiting ) and nutrient gradients across the Mediterranean appear to drive the lipid distribution of plankton communities (Popendorf et al, 2011b). Recent advances in lipid analysis via tandem ultra high pressure liquid chromatography mass spectrometry (UHPLC-MS) now allow rapid and enhanced detection of intact polar lipids (IPLs) as well as structural elucidation of their polar headgroups and alkyl chains (e.g., Wörmer et al, 2013).…”

Section: Abstract: Diacylglycerol Lipids Fatty Acids Oligotrophic Fmentioning

confidence: 99%

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Planktonic Lipidome Responses to Aeolian Dust Input in Low-Biomass Oligotrophic Marine Mesocosms

et al. 2017

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The effect and fate of dry atmospheric deposition on nutrient-starved plankton in the Eastern Mediterranean Sea (EMS; Crete, 2012) was tested by spiking oligotrophic surface seawater mesocosms (3 m 3 ) with Saharan dust (SD; 1.6 g L −1 ; 23 nmol NO x mg −1 ; 2.4 nmol PO 4 mg −1 ) or mixed aerosols (A; 1.0 g L −1 ; 54 nmol NO x mg −1 ; 3.0 nmol PO 4 mg −1 ) collected from natural and anthropogenic sources. Using high resolution liquid chromatography-mass spectrometry, the concentrations of over 350 individual lipids were measured in suspended particles to track variations in the lipidome associated with dust fertilization. Bacterial and eukaryotic intact polar lipid (IPL) biomarkers were categorized into 15 lipid classes based on headgroup identity, including four novel IPL headgroups. Bulk IPL concentrations and archaeal tetraether lipids were uncoupled with the doubling of chlorophyll concentrations that defined the stimulation response of oligotrophic plankton to SD or A amendment. However, molecular level analysis revealed the dynamics of the IPL pool, with significant additions or losses of specific IPLs following dust spikes that were consistent among treatment mesocosms. Multivariate redundancy analysis further demonstrated that the distribution of IPL headgroups and molecular modifications within their alkyl chains were strongly correlated with the temporal evolution of the plankton community and cycling of phosphate. IPLs with phosphatidylcholine, betaine, and an alkylamine-like headgroup increased in the post-stimulated period, when phosphate turnover time had decreased by an order of magnitude and phosphorus uptake was dominated by plankton >2 µm. For most IPL classes, spiking with SD or A yielded significant increases in the length and unsaturation of alkyl chains. A lack of corresponding shifts in the plankton community suggests that the biosynthesis of nitrogenous and phosphatidyl lipids may respond to physiological controls during episodic additions of dust to the EMS. Furthermore, alkyl chain distributions of IPLs containing N, P, and S invoked a bacterial source, suggesting that bacterioplankton are able to modulate these lipids in response to nutrient stress.

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Section: Ipl Signatures Of Nutrient Turnoversupporting

confidence: 80%

Section: Hierarchical Clustering Analysis Of Ipl Alkyl Chainsmentioning

confidence: 76%

Section: Ipl Signatures Of Nutrient Turnovermentioning

confidence: 99%

Section: Abstract: Diacylglycerol Lipids Fatty Acids Oligotrophic Fmentioning

confidence: 99%

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Planktonic Lipidome Responses to Aeolian Dust Input in Low-Biomass Oligotrophic Marine Mesocosms

et al. 2017

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“…DGCC was quantified based on a purified extract from cultured Thalassiosira Pseudonana (Popendorf et al, 2013). DGTS was quantified based upon the DGCC calibration subject to a scaling factor (Popendorf et al, 2011b). The internal standard mixture was run after every seven samples as a control upon instrument variability.…”

Section: Targeted Lipid Analysismentioning

confidence: 99%

Targeted and untargeted lipidomics of Emiliania huxleyi viral infection and life cycle phases highlights molecular biomarkers of infection, susceptibility, and ploidy

Hunter¹,

Frada²,

Fredricks³

et al. 2015

Front. Mar. Sci.

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Marine viruses that infect phytoplankton strongly influence the ecology and evolution of their hosts. Emiliania huxleyi is characterized by a biphasic life cycle composed of a diploid (2N) and haploid (1N) phase; diploid cells are susceptible to infection by specific coccolithoviruses, yet haploid cells are resistant. Glycosphingolipids (GSLs) play a role during infection, but their molecular distribution in haploid cells is unknown. We present mass spectrometric analyses of lipids from cultures of uninfected diploid, infected diploid, and uninfected haploid E. huxleyi. Known viral GSLs were present in the infected diploid cultures as expected, but surprisingly, trace amounts of viral GSLs were also detected in the uninfected haploid cells. Sialic-acid GSLs have been linked to viral susceptibility in diploid cells, but were found to be absent in the haploid cultures, suggesting a mechanism of haploid resistance to infection. Additional untargeted high-resolution mass spectrometry data processed via multivariate analysis unveiled a number of novel biomarkers of infected, non-infected, and haploid cells. These data expand our understanding on the dynamics of lipid metabolism during E. huxleyi host/virus interactions and highlight potential novel biomarkers for infection, susceptibility, and ploidy.

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Successful acclimation of marine diatoms Chaetoceros curvisetus/pseudocurvisetus to climate change

Vrana

Gašparović

Geček

et al. 2023

Limnology & Oceanography

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Two main parameters that structure the marine ecosystem by affecting species distribution, abundance, community structure, timing of major life cycle events, and trophic state of the ecosystem are temperature and nutrient availability. Faced with climate change, eukaryotic plankton cope at multiple levels through physiological accommodation, adaptive evolution, shift in time and/or space of habitat, and/or community responses. Thirty‐two years of our phytoplankton research indicate that Chaetoceros curvisetus/pseudocurvisetus adjust to climate change more successfully than the majority of the accompanying phytoplankton taxa in the mesotrophic part of the NW Adriatic Sea, the Mediterranean. While the abundance of the entire accompanying phytoplankton community has decreased significantly since 2003 (the period of the northern Adriatic warming and oligotrophication) compared to the previous period (1986–2003), the abundance of C. curvisetus/pseudocurvisetus remained unchanged, while its contribution to the community increased significantly. Accommodation strategies include a change in the timing of high abundance and blooms in the surface layer and successful blooming in the deeper layers during warm months. Apart from the observed in situ accommodation, physiological acclimation to warming may involve changes in photosynthesis, respiration, growth, and cell biochemistry. Here, we conducted laboratory experiments with C. pseudocurvisetus to investigate how warming affects its biochemical response through the fatty acid remodeling of phospholipid classes. Long‐term field observations and short‐term laboratory experiments suggest that marine diatoms C. curvisetus/pseudocurvisetus are potential global winners with the ability to acclimate/adapt to climate change.

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Gradients in intact polar diacylglycerolipids across the Mediterranean Sea are related to phosphate availability

Cited by 37 publications

References 42 publications

Planktonic Lipidome Responses to Aeolian Dust Input in Low-Biomass Oligotrophic Marine Mesocosms

Planktonic Lipidome Responses to Aeolian Dust Input in Low-Biomass Oligotrophic Marine Mesocosms

Targeted and untargeted lipidomics of Emiliania huxleyi viral infection and life cycle phases highlights molecular biomarkers of infection, susceptibility, and ploidy

Successful acclimation of marine diatoms Chaetoceros curvisetus/pseudocurvisetus to climate change

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