Graft gd T-cell receptor sequencing identifies public clonotypes associated to HSCT efficacy in AML patients and unravels CMV impact on repertoire distribution

The Roles of γδ T Cells in Hematopoietic Stem Cell Transplantation

“…Other studies found that Vd1 T cells recovery was correlated with CMV reactivation in HSCT recipients by using multiplex PCR system to sequence TRG and TCR delta locus (TRD) CDR3 regions or flow cytometry analysis 9,38 . Several CMV-related gd T cell clones proliferation in grafts from CMV þ donors were also observed 54 . Therefore, reconstitution of gd T cells might be involved in the outcome of CMV reactivation after transplantation.…”

“…Recently, Arruda et al used a high-throughput analysis of TCR Vg repertoire in AML patients after accepted grafts, which suggested that TCR gamma locus (TRG) clonal distribution were not associated with occurrence or absence of aGVHD 54 . However, our previous study suggested that grafts with higher proportion of CD27 þ Vd1 Tregs was associated with a lower incidence of aGVHD in G-CSF-mobilized allogeneic peripheral blood stem cell transplantation recipients 7 .…”