Grafted megaporous materials as ion‐exchangers for bioproduct adsorption

Abstract

Megaporous chromatographic materials were manufactured by a three-step procedure, including backbone synthesis, chemical grafting, and introduction of ion-exchange functionality. The backbone of the adsorbent cylindrical bodies was prepared by polymerization of methacrylic acid and poly(ethylene glycol) diacrylate at sub-zero temperatures. Grafting was performed employing glycidyl methacrylate and a chemical initiator, cerium ammonium nitrate. The degree of grafting was adjusted by modifying the concentration of the initiator in the reaction mixture to a range of values (23, 39, 62, 89, and 105%). Further, the pendant epoxy-groups generated by the previous step were reacted to cation- and anion-exchanging moieties utilizing known chemical routes. Infrared spectroscopy studies confirmed the incorporation of epoxy and ion-exchanger groups to the backbone material. Optimized materials were tested for chromatography applications with model proteins; the dynamic binding capacity, as recorded at 10% breakthrough and 2.0 × 10(-4) m/s superficial velocity, were 350 and 58 mg/g for the cation-exchanger and the anion-exchanger material, respectively. These results may indicate that long tentacle-type polymer brushes were formed during grafting therefore increasing the ability of the megaporous body to efficiently capture macromolecules.

A megaporous material harbouring a peptide ligand for affinity IgG purification

“…The material was dried to constant weight in a vacuum oven to estimate its dried weight, which was required in the next steps for physical characterization. It was proved in our previous study that the material was physically stable under the conditions described in this paper and possessed the property to re‐swell in water or buffer solutions . The later property helped to obtain a homogenous packing of the material in chromatography columns.…”

“…The methods we used in this work are known to promote the formation of “tentacles” or “polymer brushes” . Furthermore, we recently published a paper using the same MP‐EP used in this work and was further reacted to cation‐ and anion‐exchanging moieties utilizing known chemical routes . IR spectroscopy studies confirmed the incorporation of epoxy and ion‐exchanger groups to the backbone material.…”

“…In this work, a megaporous material was synthesised by copolymerization, using methacrylic acid (MAA) as functional monomer and poly(ethylene glycol) diacrylate as cross‐linker to produce the so‐called MA‐CM. This expanded material showed excellent mechanical stability and good convective flow characteristics . Subsequently, MA‐CM was grafted using glycidyl methacrylate (GMA) to incorporate epoxy pendant groups within the structure.…”

Porous Polymers Derived from Octavinylsilsesquioxane by Cationic Polymerization

“…The past decade has seen a large number of significant breakthroughs in the design and synthesis of novel porous materials, driven by the rapid growth of emerging applications . Especially in the chemical field, the application of porous materials is more extensive, such as catalysis, ion exchange, adsorption and separation, sensing, chromatography, and energy storage . The emergence of such new technological applications requires a higher level of control over the properties of porous materials and the need to create simple and efficient ways to prepare porous materials has steadily increased over recent years.…”