Grafted twig rot on Citrus sinensis caused by a member of the Fusarium solani species complex

Liu, Hai Feng; Zhou, Jun; Liao, Jing; Yi, Ji Ping; Fang, Dong; Deng, Jun

doi:10.1080/07060661.2019.1633412

Cited by 11 publications

(12 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…A modified version of the CTAB (cetyltriethylammnonium bromide) method was used to extract the DNA of the endophyte isolates [15]. Additionally, the extracted DNA was used as template for polymerase chain reaction (PCR) amplification.…”

Section: Classification Of Endophyte Isolatesmentioning

confidence: 99%

Screening, Identification, and Optimization of Fermentation Conditions of an Antagonistic Endophyte to Wheat Head Blight

Zhang

Zhu

et al. 2019

Agronomy

View full text Add to dashboard Cite

Fusarium Head Blight (FHB, scab) is a destructive fungal disease that causes extensive yield and quality losses in wheat and other small cereals. Biological control of FHB is considered to be an alternative disease management strategy that is environmentally benign, durable, and compatible with other control measures. In this study, to screen antagonistic bacteria with the potential to manage FHB, 113 endophytes were isolated from the stems, leaves, panicles, and roots of wheat. Among them, six strains appeared to effectively inhibit Fusarium graminearum growth and one isolate, XS-2, showed a highly antagonistic effect against FHB. An in vitro antagonistic test of XS-2 on wheat heads confirmed that XS-2 could suppress the disease severity of FHB. The 16S rDNA sequence analysis revealed that XS-2 is a strain of Bacillus amyloliquefaciens. Antagonistic spectrum analyses showed that XS-2 had antagonistic effects against two and four types of cotton and fruit tree pathogens, respectively. The fermentation condition assays showed that glucose and peptone are the most suitable nutrient sources for XS-2, and that the optimal pH value and temperature for fermentation were 7.4 and 28 °C, respectively. Our study indicates that XS-2 has a good antagonistic effect on FHB and lays a theoretical foundation for the application of the strain as a biological agent in the field to control FHB.

show abstract

Section: Classification Of Endophyte Isolatesmentioning

confidence: 99%

Screening, Identification, and Optimization of Fermentation Conditions of an Antagonistic Endophyte to Wheat Head Blight

Zhang

Zhu

et al. 2019

Agronomy

View full text Add to dashboard Cite

show abstract

“…To construct the phylogenetic tree and identify the clade where the consensus sequence is grouped (Fusarium sp. Isolated Veracruz), the sequences of the translation elongation factor 1 alpha (TEF-1ɑ) of the species complex comprising Fusarium solani, F. oxysporum, and F. citricola, which affect citrus plants, were downloaded from GenBank (Liu et al, 2020;Sandoval-Denis et al, 2018;Yaseen and D'Onghia, 2012), as well as the sequences that were most similar to those of the species under study (Table 1). The species Mini Kit (Qiagen), de acuerdo con las instrucciones del fabricante.…”

Section: Aislamiento Y Purificaciónmentioning

confidence: 99%

“…Para realizar el árbol filogenético e identificar el clado donde se agrupa la secuencia consenso (Fusarium sp. aislado Veracruz), se descargaron del GenBank las secuencias del factor de elongación de traducción 1 alfa (TEF-1ɑ) del complejo de especies de Fusarium solani, F. oxysporum y F. citricola que afectan al cultivo de cítricos (Liu et al, 2020;Sandoval-Denis et al, 2018;Yaseen y D'Onghia, 2012), así como las secuencias que tuvieron mayor similitud con la especie en estudio Mexican Journal of PHYTOPATHOLOGY Revista Mexicana de FITOPATOLOGÍA Advance online publication, 2021…”

Section: Aislamiento Y Purificaciónunclassified

“…GQ425229). The phylogenetic tree yielded four highly contrasting lineages, three of which corresponded to the species complex formed by F. oxysporum, F. citricola, and F. solani, currently known as citrus pathogens (Liu et al, 2020;Sandoval-Denis et al, 2018;Yaseen and D'Onghia, 2012). The fourth lineage grouped Fusarium sp.…”

Section: Molecular Identification and Phylogenetic Treementioning

confidence: 99%

See 1 more Smart Citation

Fusarium sp., causal agent of vascular wilt in citrus and its sensitivity to fungicides

Robles-Yerena¹,

Leyva-Mir²,

Camacho-Tapia³

et al. 2021

RMF

View full text Add to dashboard Cite

Citrus wilt is a disease of recent appearance in the northern area of Veracruz that causes economic losses to producers in the region. The present work aimed to identify the causative agent of this disease and evaluate different fungicides to determine its in vitro sensitivity. A fungus was consistently isolated in plants with wilt symptoms; it was morphologically identified indifferent culture media and molecularly identified by PCR using the EF1-728F/EF1-986R primers. The fungus was inoculated in three varieties of citrus under greenhouse conditions. The sensitivity test was carried out with the fungicides chlorothalonil, benomyl thiabendazole, prochloraz, and a biological agent (Bacillus subtilis) at different concentrations, plus a negative control. Fusarium sp. (Accession No. MW438335) was morphologically and molecularly identified as the causal agent of vascular wilt in citrus fruits, causing growth retardation, decreased number of roots, wilting of the apical bud, and necrosis in the vascular system of the three varieties inoculated. The most effective fungicides in inhibiting mycelial growth were thiabendazole, prochloraz, and the biological agent Bacillus subtilis.

show abstract

“…However, citrus plantations are threatened by several pathogens of which the Fusarium species, mainly F. solani , is causing citrus dry root rot (DRR) disease, which is responsible for significant economic loss [ 1 , 3 , 4 ]. In addition, Fusarium species can cause other serious diseases on citrus plantation such as twig rot, decline dieback, twig blight, and vascular wilt, thereby are a major threat to citrus production worldwide [ 1 , 5 , 6 ]. Fusarium sensu latu was recently segregated into several Fusarium -like genera (i.e., Bisifusarium [ Fusarium dimerum specie complex (SC)], Neocosmospora [ Fusarium solani SC] and Rectifusarium [ Fusarium ventricosum SC] [ 7 ].…”

Section: Introductionmentioning

confidence: 99%

Potential Role of Rhizobacteria Isolated from Citrus Rhizosphere for Biological Control of Citrus Dry Root Rot

Ezrari

Mhidra

Radouane

et al. 2021

Plants

View full text Add to dashboard Cite

Citrus trees face threats from several diseases that affect its production, in particular dry root rot (DRR). DRR is a multifactorial disease mainly attributed to Neocosmospora (Fusarium) solani and other several species of Neocosmospora and Fusarium spp. Nowadays, biological control holds a promising control strategy that showed its great potential as a reliable eco-friendly method for managing DRR disease. In the present study, antagonist rhizobacteria isolates were screened based on in vitro dual culture bioassay with N. solani. Out of 210 bacterial isolates collected from citrus rhizosphere, twenty isolates were selected and identified to the species level based on the 16S rRNA gene. Molecular identification based on 16S rRNA gene revealed nine species belonging to Bacillus, Stenotrophomonas, and Sphingobacterium genus. In addition, their possible mechanisms involved in biocontrol and plant growth promoting traits were also investigated. Results showed that pectinase, cellulose, and chitinase were produced by eighteen, sixteen, and eight bacterial isolates, respectively. All twenty isolates were able to produce amylase and protease, only four isolates produced hydrogen cyanide, fourteen isolates have solubilized tricalcium phosphate, and ten had the ability to produce indole-3-acetic acid (IAA). Surprisingly, antagonist bacteria differed substantially in their ability to produce antimicrobial substances such as bacillomycin (five isolates), iturin (ten isolates), fengycin (six isolates), surfactin (fourteen isolates), and bacteriocin (subtilosin A (six isolates)). Regarding the PGPR capabilities, an increase in the growth of the bacterial treated canola plants, used as a model plant, was observed. Interestingly, both bacterial isolates Bacillus subtilis K4-4 and GH3-8 appear to be more promising as biocontrol agents, since they completely suppressed the disease in greenhouse trials. Moreover, these antagonist bacteria could be used as bio-fertilizer for sustainable agriculture.

show abstract

Grafted twig rot on Citrus sinensis caused by a member of the Fusarium solani species complex

Cited by 11 publications

References 21 publications

Screening, Identification, and Optimization of Fermentation Conditions of an Antagonistic Endophyte to Wheat Head Blight

Screening, Identification, and Optimization of Fermentation Conditions of an Antagonistic Endophyte to Wheat Head Blight

Fusarium sp., causal agent of vascular wilt in citrus and its sensitivity to fungicides

Potential Role of Rhizobacteria Isolated from Citrus Rhizosphere for Biological Control of Citrus Dry Root Rot

Contact Info

Product

Resources

About