Grafting as a strategy to increase flowering of cassava

Souza, Leonardo Silva; Diniz, Rafael Parreira; Neves, Reizaluamar de Jesus; Alves, Alfredo Augusto Cunha; Oliveira, E. J. de

doi:10.1016/j.scienta.2018.06.070

Cited by 41 publications

(19 citation statements)

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“…Moreover, the effect of grafting on the precious floral induction has been reported, up to now, in apple, where the M9 rootstock (a spontaneous mutant overexpressing FT genes) induces early flowering in juvenile apple scion (Foster et al, 2014). Similar results have been reported in Arabidopsis, tomato, and cassava (Lifschitz et al, 2006;Corbesier et al, 2007;Notaguchi et al, 2008;Silva Souza et al, 2018). In blueberry, the phenomenon was artificially reproduced using an FT transgenic rootstock and a non-transgenic scion (Song et al, 2019b).…”

Section: Inducing Early Flowering As a Strategy To Shorten The Long Jsupporting

confidence: 57%

New Plant Breeding Techniques in Citrus for the Improvement of Important Agronomic Traits. A Review

et al. 2020

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New plant breeding techniques (NPBTs) aim to overcome traditional breeding limits for fruit tree species, in order to obtain new varieties with improved organoleptic traits and resistance to biotic and abiotic stress, and to maintain fruit quality achieved over centuries by (clonal) selection. Knowledge on the gene(s) controlling a specific trait is essential for the use of NPBTs, such as genome editing and cisgenesis. In the framework of the international scientific community working on fruit tree species, including citrus, NPBTs have mainly been applied to address pathogen threats. Citrus could take advantage of NPBTs because of its complex species biology (seedlessness, apomixis, high heterozygosity, and long juvenility phase) and aptitude for in vitro manipulation. To our knowledge, genome editing in citrus via transgenesis has successful for induced resistance to Citrus bacterial canker in sweet orange and grapefruit using the resistance gene CsLOB1. In the future, NPBTs will also be used to improve fruit traits, making them healthier. The regeneration of plants following the application of NPBTs is a bottleneck, making it necessary to optimize the efficiency of current protocols. The strengths and weaknesses of using explants from young in vitro plantlets, and from mature plants, will be discussed. Other major issues addressed in this review are related to the requirement for marker-free systems and shortening the long juvenility phase. This review aims to summarize methods and approaches available in the literature that are suitable to citrus, focusing on the principles observed before the use of NPBTs.

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Section: Inducing Early Flowering As a Strategy To Shorten The Long Jsupporting

confidence: 57%

New Plant Breeding Techniques in Citrus for the Improvement of Important Agronomic Traits. A Review

et al. 2020

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“…Scions obtained from non-transgenic hardened plants of cultivars 60444 and TME 204 were cleft grafted onto transgenic rootstocks according to Souza et al [7]. Briefly, a 'V' shaped incision aligned to the cambial region was made in the stem of 28-days old FT overexpressing transgenic rootstocks from tissue culture.…”

Section: Plant Establishment In the Greenhouse And Graftingmentioning

confidence: 99%

“…Crossing blocks can be established in geographical regions that favour flowering, while varied planting times can help synchronize flowering of parental genotypes [5]. Foliar application of plant growth regulators [6] and grafting onto rootstocks obtained from high flowering varieties are also techniques employed to improve flowering and allow controlled breeding programs [4,7]. Temperature, day length (photoperiod), levels of sugar, nitrates and plant growth regulators have been identified as triggers for flowering in Arabidopsis thaliana and other plant species [8][9][10].…”

Section: Introductionmentioning

confidence: 99%

“…Functions of native FT genes in cassava remain largely unknown. Advances in genomics and biotechnology techniques such as transgenic and gene editing provide promise to address this challenge [7]. The objective of this study was to investigate the role of native FT-like gene MeFT1 in regulation of cassava flowering and storage root development, thereby contributing towards an avenue for shortened breeding cycle and increased yield for this important staple crop.…”

Section: Introductionmentioning

confidence: 99%

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Transgenic overexpression of endogenous FLOWERING LOCUS T-like gene MeFT1 produces early flowering in cassava

et al. 2020

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Endogenous FLOWERING LOCUS T homolog MeFT1 was transgenically overexpressed under control of a strong constitutive promoter in cassava cultivar 60444 to determine its role in regulation of flowering and as a potential tool to accelerate cassava breeding. Early profuse flowering was recorded in-vitro in all ten transgenic plant lines recovered, causing eight lines to die within 21 days of culture. The two surviving transgenic plant lines flowered early and profusely commencing as soon as 14 days after establishment in soil in the greenhouse. Both transgenic lines sustained early flowering across the vegetative propagation cycle, with first flowering recorded 30-50 days after planting stakes compared to 90 days for non-transgenic controls. Transgenic plant lines completed five flowering cycles within 200 days in the greenhouse as opposed to twice flowering event in the controls. Constitutive overexpression of MeFT1 generated fully mature male and female flowers and produced a bushy phenotype due to significantly increased flowering-induced branching. Flower induction by MeFT1 overexpression was not graft-transmissible and negatively affected storage root development. Accelerated flowering in transgenic plants was associated with significantly increased mRNA levels of MeFT1 and the three floral meristem identity genes MeAP1, MeLFY and MeSOC1 in shoot apical tissues. These findings imply that MeFT1 encodes flower induction and triggers flowering by recruiting downstream floral meristem identity genes. OPEN ACCESS Citation: Odipio J, Getu B, Chauhan RD, Alicai T, Bart R, Nusinow DA, et al. (2020) Transgenic overexpression of endogenous FLOWERING LOCUS T-like gene MeFT1 produces early flowering in cassava. PLoS ONE 15(1): e0227199.

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“…Pina et al, 2012 Furthermore, incompatibility between scion rootstock species may induce a decrease in shoot growth and water transport, which may lead to the death of the plant ( ). Graft in-Davis and Perkins-Veazie, 2008 compatibility generally occurs at the early stages of graft development when vascular connections are forming; however, symptoms may manifest at later growth stages such as low plant development related to physiological di erences in stem diameter, which impairs the normal ff fl fi ow of photoassimilates and the ligni cation of grafted tissues (Souza et al, 2018), thereby decreasing the hydraulic conductivity of the graft union ( ; ; Solari et al, 2006Gascó et al, 2007Tworkoski and Fazio, 2015. These symptoms appear during the plant fruiting period, when the plant is subject to a high demand for water transport (Martínez-Ballesta et al, 2010).…”

Section: Vascular Alignment Of Anatomical Tissues In Grafted Plantsmentioning

confidence: 99%

An overview of grafting re-establishment in woody fruit species

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Amaro

Pina

et al. 2019

Scientia Horticulturae

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Grafting as a strategy to increase flowering of cassava

Cited by 41 publications

References 30 publications

New Plant Breeding Techniques in Citrus for the Improvement of Important Agronomic Traits. A Review

New Plant Breeding Techniques in Citrus for the Improvement of Important Agronomic Traits. A Review

Transgenic overexpression of endogenous FLOWERING LOCUS T-like gene MeFT1 produces early flowering in cassava

An overview of grafting re-establishment in woody fruit species

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