Grafting of Biocompatible Polymers on DLC Thin Films by Plasma Irradiation-Post Polymerization Technique for Application of Biomedical Devices and Cell Microarrays

Solution Properties and Potential Biological Applications of Zwitterionic Poly(ε-N-methacryloyl-l-lysine)

“…Zwitterionic polymers are known for their biocompatibility, , antifouling properties, , and low protein adsorption from human blood. ,− Therefore, poly(ε- N -methacryloyl- l -lysine) might be a suitable candidate as a “nano carrier” for biomedical applications like gene transfection or targeted delivery of drugs. The requirements for such a nano carrier are low toxicity and no severe interactions with biological media like blood serum, which in the worst case could lead to the formation of large aggregates.…”

“…73 Potential Application in Biological Systems. Zwitterionic polymers are known for their biocompatibility, 47,74 antifouling properties, 56,57 and low protein adsorption from human blood. 48,53−55 Therefore, poly(ε-N-methacryloyl-Llysine) might be a suitable candidate as a "nano carrier" for biomedical applications like gene transfection or targeted delivery of drugs.…”

“…Although polymers with various amino acids in the side chains are frequently reported, − zwitterionic polymers incorporating amino acids in the side chains − have not received much attention although such polymers may be expected to show a good biocompatibility and low protein adsorption − as known for other polybetaines. − Long ago, Morawetz and Sammak have described the synthesis of poly(ε- N -methacryloyl- l -lysine) (PMALys) and investigated the Cu 2+ chelate formation. Later, chelates with other transition metal ions such as Ni 2+ and Zn 2+ were investigated. , …”

Preparation of zwitterionic random and block poly(N-α-acrylamide-l-lysine-co-2-methacryloyloxyethyl phosphorylcholine) copolymers and their effect on fibrinolytic activity

“…Furthermore, we reported that poly(LysAA) and poly(LysMA) enhanced fibrinolytic activity based on strong interactions with the lysine binding sites (LBS) of Plg and t-PA via L-lysine in the polymer side chain. [7][8][9][10] Poly(2methacryloyloxyethyl phosphorylcholine) (MPC) is a strong, bioinert polymer that has been used in a wide range of applications in nanomedicine and bioengineering, in medical devices and sensors, and as an antifouling material. 11,12 The zwitterionic structures of LysAA and LysMA resemble that of the side chain of MPC and suppress the adsorption of serum protein and enhance the fibrinolytic activity by Plg and t-PA binding moieties without the need to pre-coat with fibrinolytic factors.…”

“…The binding of Plg to a cell surface stimulates plasmin (Plm) generation by plasminogen activator, in contrast to Plg in solution. 14,15 In this study, prior to immobilizing proteins on a material surface and evaluating the biocompatibility of the surface, 8,9 we investigated the effect of MPC segments containing a lipid bilayer component on the fibrinolytic promoting effect of poly(LysAA). The materials prepared in this study were intended for practical use in medical applications and were prepared by random radical polymerization to avoid the use of initiators of toxicological concern.…”