Grafting of Genetically Engineered Plants

Song, Guo Qing; Walworth, Aaron E.; Loescher, Wayne H.

doi:10.21273/jashs.140.3.203

Cited by 24 publications

(24 citation statements)

References 119 publications

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“…For example, in blueberry, graft transmissible flowering was reported following grafting of non-transgenic scions onto transgenic rootstocks overexpressing FT. This success was attributed to graft transmission of VcFT plus phytohormones ( [44]). Further investigation is required to assess if a similar process is functioning in cassava.…”

Section: Discussionmentioning

confidence: 99%

Transgenic overexpression of endogenous FLOWERING LOCUS T-like gene MeFT1 produces early flowering in cassava

et al. 2020

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Endogenous FLOWERING LOCUS T homolog MeFT1 was transgenically overexpressed under control of a strong constitutive promoter in cassava cultivar 60444 to determine its role in regulation of flowering and as a potential tool to accelerate cassava breeding. Early profuse flowering was recorded in-vitro in all ten transgenic plant lines recovered, causing eight lines to die within 21 days of culture. The two surviving transgenic plant lines flowered early and profusely commencing as soon as 14 days after establishment in soil in the greenhouse. Both transgenic lines sustained early flowering across the vegetative propagation cycle, with first flowering recorded 30-50 days after planting stakes compared to 90 days for non-transgenic controls. Transgenic plant lines completed five flowering cycles within 200 days in the greenhouse as opposed to twice flowering event in the controls. Constitutive overexpression of MeFT1 generated fully mature male and female flowers and produced a bushy phenotype due to significantly increased flowering-induced branching. Flower induction by MeFT1 overexpression was not graft-transmissible and negatively affected storage root development. Accelerated flowering in transgenic plants was associated with significantly increased mRNA levels of MeFT1 and the three floral meristem identity genes MeAP1, MeLFY and MeSOC1 in shoot apical tissues. These findings imply that MeFT1 encodes flower induction and triggers flowering by recruiting downstream floral meristem identity genes. OPEN ACCESS Citation: Odipio J, Getu B, Chauhan RD, Alicai T, Bart R, Nusinow DA, et al. (2020) Transgenic overexpression of endogenous FLOWERING LOCUS T-like gene MeFT1 produces early flowering in cassava. PLoS ONE 15(1): e0227199.

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Section: Discussionmentioning

confidence: 99%

Transgenic overexpression of endogenous FLOWERING LOCUS T-like gene MeFT1 produces early flowering in cassava

et al. 2020

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“…The underlying mechanisms for producing a successful graft have been studied for decades, of which how rootstocks and scions communicate with each other remains an unresolved research problem. Thus far, many metabolites, protein, mRNA, and small RNAs, including microRNAs (miRNAs) and small interfering RNAs (siR-NAs) are all suggested to be potential molecular signals facilitating rootstock-scion communications [4][5][6][7][8][9][10][11][12][13].…”

Section: Introductionmentioning

confidence: 99%

Transfer of endogenous small RNAs between branches of scions and rootstocks in grafted sweet cherry trees

Zhao

Zhong²,

Song

2020

PLoS ONE

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Grafting is a well-established agricultural practice in cherry production for clonal propagation, altered plant vigor and architecture, increased tolerance to biotic and abiotic stresses, precocity, and higher yield. Mobile molecules, such as water, hormones, nutrients, DNAs, RNAs, and proteins play essential roles in rootstock-scion interactions. Small RNAs (sRNAs) are 19 to 30-nucleotides (nt) RNA molecules that are a group of mobile signals in plants. Rootstock-to-scion transfer of transgene-derived small interfering RNAs enabled virus resistance in nontransgenic sweet cherry scion. To determine whether there was longdistance scion-to-rootstock transfer of endogenous sRNAs, we compared sRNAs profiles in bud tissues of an ungrafted 'Gisela 6' rootstock, two sweet cherry 'Emperor Francis' scions as well as their 'Gisela 6' rootstocks. Over two million sRNAs were detected in each sweet cherry scion, where 21-nt sRNA (56.1% and 55.8%) being the most abundant, followed by 24-nt sRNAs (13.1% and 12.5%). Furthermore, we identified over three thousand sRNAs that were potentially transferred from the sweet cherry scions to their corresponding rootstocks. In contrast to the sRNAs in scions, among the transferred sRNAs in rootstocks, the most abundant were 24-nt sRNAs (46.3% and 34.8%) followed by 21-nt sRNAs (14.6% and 19.3%). In other words, 21-nt sRNAs had the least transferred proportion out of the total sRNAs in sources (scions) while 24-nt had the largest proportion. The transferred sRNAs were from 574 cherry transcripts, of which 350 had a match from the Arabidopsis thaliana standard protein set. The finding that "DNA or RNA binding activity" was enriched in the transcripts producing transferred sRNAs indicated that they may affect the biological processes of the rootstocks at different regulatory levels. Overall, the profiles of the transported sRNAs and their annotations revealed in this study facilitate a better understanding of the role of the long-distance transported sRNAs in sweet cherry rootstock-scion interactions as well as in branch-to-branch interactions in a tree.

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“…It involves the combination a transgenic rootstock with a non-transgenic scion (Haroldsen et al 2012;Lev-Yadun and Sederoff 2001). In a transgrafted plant, the transgenic rootstock synthesizes both immobile transgene product (ITP), which is very unlikely to be transported through the graft union, and mobile transgene products (MTP) that is capable of crossing the graft union (Song et al 2015). Examples of MTP are signal molecules which are involved in either long-or short-distance transport include RNA (mRNA, siRNA, miRNA), peptides and protein (Liu et al 2017;Luo et al 2018;Zhao and Song 2014).…”

Section: Transgraftingmentioning

confidence: 99%

Development of genetically modified citrus plants for the control of citrus canker and huanglongbing

Soares

Tanwir

Grosser

2020

Trop. plant pathol.

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Citrus cultivation is challenging due to the plethora of abiotic and biotic stresses faced by the crop. In recent years, production has been severely affected by diseases such as citrus canker and huanglongbing (HLB). Disease management is hampered as there is no field resistance to these diseases in any of the important commercially planted varieties. Traditionally, conventional breeding approaches have been applied for the improvement of the susceptible cultivars; however, this technique is laborious and time consuming. Genetic transformation of citrus allows for the rapid integration of novel genes into the plant's genome to develop disease-resistant transgenic plants. Therefore, efforts have been made to utilize genetic engineering tools to develop genetically modified citrus that are resistant to citrus canker and HLB. This review summarizes the major achievements made in the development of citrus canker and HLB tolerance using transgenic technologies.

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Grafting of Genetically Engineered Plants

Cited by 24 publications

References 119 publications

Transgenic overexpression of endogenous FLOWERING LOCUS T-like gene MeFT1 produces early flowering in cassava

Transgenic overexpression of endogenous FLOWERING LOCUS T-like gene MeFT1 produces early flowering in cassava

Transfer of endogenous small RNAs between branches of scions and rootstocks in grafted sweet cherry trees

Development of genetically modified citrus plants for the control of citrus canker and huanglongbing

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