Grafting of Methyl Methacrylate onto Gelatin Initiated by Tri-Butylborane—2,5-Di-Tert-Butyl-p-Benzoquinone System

Abstract

Graft gelatin and poly(methyl methacrylate) copolymers were synthesized in the presence of the tributylborane—2,5-di-tert-butyl-p-benzoquinone (2,5-DTBQ) system. The molecular weight parameters and morphology of the polymer indicate that it has a cross-linked structure. Obtained data confirm the simultaneous formation of a copolymer in two ways: “grafting from” and “grafting to”. It leads to the cross-linked structure of a copolymer. This structure was not obtained for copolymers synthesized in the presence of other initiating systems: azobisisobutyronitrile; tributylborane; azobisisobutyronitrile and tributylborane; azobisisobutyronitrile, tributylborane, and 2,5-di-tert-butyl-p-benzoquinone. In these cases, the possibility of the formation of the copolymer, simultaneously in two ways, was excluded. Graft gelatin and poly(methyl methacrylate) copolymers synthesized in the presence of the tributylborane—2,5-di-tert-butyl-p-benzoquinone system are promising in terms of their use in scaffold technologies due to the three-dimensional mesh structure, providing a high regenerative potential of materials.

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“…Thus, the results of the study of the chemical properties and cytotoxicity of graft copolymers formed from methyl methacrylate with cod collagen using radical initiator azobisisobutyronitrile, demonstrate the promising character of the new material for the production of medical wound healing coatings and scaffolds. Previously, graft copolymers of acrylic monomers on cod collagen have been formed using photocatalysis with rather complex enhancement using RbTe 1.5 W 0.5 O 6 oxide and irradiation with visible light λ=400-700nm [31][32][33] or with catalysts based on trialkylboranes [34,35]. The advantages of these initiators include their ability to run the processes at room temperature.…”

“…In this regard, research is being conducted to create hybrid materials based on collagen and synthetic polymers for regenerative medicine [20-The purpose of this study is to derive data on the chemical properties and cytotoxicity of graft copolymers using scaffold precursors based on MMA and CC with enhancement by the frequently-used radical initiator AIBN. In previous studies, the author obtained graft copolymers of acrylic monomers based on cod collagen using photocatalysis, either involving rather complex RbTe 1.5 W 0.5 O 6 oxide enhancement and irradiation with visible light λ=400-700nm at a temperature of 20-25 °C [31][32][33], or with catalysts based on trialkylboranes [34,35]. The visualized morphology of the lyophilized samples of such graft copolymers allowed their morphological peculiarities to be established and compared to the original collagen, indicating the inclusion of synthetic polymer fragments in the fibrillar collagen structure.…”

Scaffold Chemical Model Based on Collagen—Methyl Methacrylate Graft Copolymers

“…In this case, the resulting product must have a cross-linked structure required in scaffold technologies. This was first implemented in our work [ 36 ], which involved the synthesis of gelatin and methyl methacrylate copolymers in the presence of tributylborane and 2,5-di-tert-butyl- p -benzoquinone. In this case, the chain begins to grow on the borated collagen (“grafting from”) and breaks off on the borated collagen (“grafting to”).…”

“…This work aimed to create a chemical model of scaffold construction based on graft-copolymers of collagen and methyl methacrylate using tributyl borane and p -quinones. For this, it was necessary to complete the following tasks: Obtain copolymers of methyl methacrylate and fish collagen in the presence of tributyl borane and several p -quinones differing in their structure and reactivity, in addition to the previously obtained data [ 36 ]; Characterize the obtained products using the methods of gel-penetrating chromatography and scanning electron microscopy; Evaluate the propensity of copolymers for biodegradation under the action of enzymes in comparison with collagen to understand the prospects of their use as precursors of scaffolds; Conduct a cytotoxicity examination; Form a chemical model of scaffold construction based on collagen and methyl methacrylate graft-copolymers using tributyl borane and p -quinones. …”

“…Obtain copolymers of methyl methacrylate and fish collagen in the presence of tributyl borane and several p -quinones differing in their structure and reactivity, in addition to the previously obtained data [ 36 ];…”