Grafting of Polypeptides on Solid Substrates by Initiation of N-Carboxyanhydride Polymerization by Amino-Terminated Self-Assembled Monolayers

Abstract

Stamm, M. (1997). Grafting of polypeptides on solid substrates by initiation of N-carboxyanhydride polymerization by aminoterminated self-assembled monolayers. Langmuir, 13(4), 723-728. DOI: 10.1021/la960467g Copyright Other than for strictly personal use, it is not permitted to download or to forward/distribute the text or part of it without the consent of the author(s) and/or copyright holder(s), unless the work is under an open content license (like Creative Commons).Take-down policy If you believe that this document breaches copyright please contact us providing details, and we will remove access to the work immediately and investigate your claim.Downloaded from the University of Groningen/UMCG research database (Pure): http://www.rug.nl/research/portal. For technical reasons the number of authors shown on this cover page is limited to 10 maximum. Volker Erb and Manfred StammMax-Plank-Institut fü r Polymerforschung, Ackermannweg 10, 55128 Mainz, Germany Received May 13, 1996. In Final Form: December 9, 1996 A series of mixed self-assembled monolayers of functionalized (Br(CH2)11SiCl3) and unfunctionalized (CH3(CH2)10SiCl3) alkyltrichlorosilanes of different compositions have been prepared on bulk silicon substrates. By in situ modification of these monolayers the bromo end groups were transformed to amino end groups as shown by X-ray photoelectron spectroscopy measurements. The change of hydrophilicity was monitored by water contact angle measurements, showing the expected decrease of contact angles with increase amino group content on the surface. These substrates were used to initiate Ncarboxyanhydrides in dioxane to yield R-helical polypeptides grafted from the surface. The thicknesses of the obtained polymer layers were measured with ellipsometry and X-ray reflectometry. Fourier transform infrared measurements confirm that the grafted polymers are in the R-helical conformation.

On-chip immunoprecipitation for protein purification

“…A 5% APTES solution in 95% ethanol/5% dH 2 . 28 As the results obtained using 5% APTES solution in 95% ethanol/5% dH 2 O were in the closest agreement with the literature, this method was employed for silanisation.…”

Sensing Immobilized Molecules of Streptavidin on a Silicon Surface by Maldi-Tof Mass Spectrometry and Fluorescence Microscopy

“…N-Alkylphthalimide derivatives can be converted into amines using the Ing-Manske modification of the Gabriel reaction 23,32 . The monolayers were reacted with a 5% solution of hydrazine in ethanol at room temperature for 48 h. This results in a decrease in the water contact angle (a contact angle of 82º for the N-(ω-Undecylenyl)-Phthalimide functionalized surface changes to 65º for the amino-terminated undecyl monolayer surface) which indicates the removal of the phthalimide groups and the emergence of the hydrophilic groups 23,33 . Direct detection of the amino groups in the monolayers is difficult with IR spectroscopy 23 due to the formation of hydrogen bonds with adsorbed water.…”

Recent developments in the site‐specific immobilization of proteins onto solid supports

“…These molecules react faster with the silanol groups of the surface and due to the selfassembling character of the long alkyl chains, they are able to form densely packed highly ordered monolayers with solidstate like properties. 38,39 Sulfur-and selenium-containing compounds can also be used for the modification of substrates based on several transition metals (Au, Ag, and Pt) 39,40 or semiconductor materials (e.g., Ag 2 S, CdS, and CdSe). 14 The most studied system, however, uses alkanethiols on gold surfaces.…”