Grafting of tomato mutants onto potato rootstocks: An approach to study leaf-derived signaling on tuberization

Peres, Lázaro Eustáquio Pereira; Carvalho, Rogério Falleiros; Zsögön, Agustín; Bermudez-Zambrano, O. D.; Robles, Walter Geremias Rodriguez; Tavares, S. C. C. de H.

doi:10.1016/j.plantsci.2005.05.017

Cited by 32 publications

(30 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Compared to self‐grafted tomato, Sl/St heterografting had no significant effect on morphological parameters (plant height, stem diameter, leaf number, leaf area and shoot biomass) or physiological parameters (chlorophyll content, hormone level and photosynthetic rate) of the tomato scion (Tables S2 and S4). These results corresponded well with previous studies indicating that tomato scions are able to grow vigorously (Peres et al ), which suggests that essential growth substances can be transported across the grafting union from the potato rootstock to control tomato morphogenesis.…”

Section: Discussionsupporting

confidence: 92%

“…Within the context of the tomato/potato heterografting system, both the rootstock and scion are sink tissues and eventually form fruits and tubers. Peres et al () found that tomato scions are always less effective in promoting tuberization when grafted onto potato rootstocks. Some graft‐induced phenotypic responses have been well‐characterized, and several hypotheses to explain these differences have been proposed.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Comprehensive transcriptome profiling and phenotyping of rootstock and scion in a tomato/potato heterografting system

Zhang

Mao

Wang

et al. 2018

Physiologia Plantarum

View full text Add to dashboard Cite

Tomato/potato heterografting‐triggered phenotypic variations are well documented, yet the molecular mechanisms underlying grafting‐induced phenotypic processes remain unknown. To investigate the phenotypic and transcriptomic responses of grafting parents in heterografting in comparison with self‐grafting, tomato (Sl) was grafted onto potato rootstocks (St), and comparative phenotyping and transcriptome profiling were performed. Phenotypic analysis showed that Sl/St heterografting induced few phenotypic changes in the tomato scion. A total of 209 upregulated genes were identified in the tomato scion, some of which appear to be involved in starch and sucrose biosynthesis. Sl/St heterografting induced several modifications in the potato rootstocks (St‐R), stolon number, stolon length and tuber number decreased significantly, together with an increase in GA3 content of stolon and tuber, compared with self‐grafted potato (St‐WT). These results indicate that the tomato scion is less effective at producing substances or signals to induce tuberization but promotes stolon development into aerial stems and sprouting. RNA‐Seq data analysis showed that 1529 genes were upregulated and 1329 downregulated between St‐WT and St‐R; some of these genes are involved in plant hormone signal transduction, with GID1‐like gibberellin receptor (StGID1) and DELLA protein (StDELLA) being upregulated. Several genes in auxin, abscisic acid and ethylene pathways were differentially expressed as well. Various hormone signals engage in crosstalk to regulate diverse phenotypic events after grafting. This work provides abundant transcriptome profile data and lays a foundation for further research on the molecular mechanisms underlying RNA‐based interactions between rootstocks and scions after tomato/potato heterografting.

show abstract

Section: Discussionsupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Comprehensive transcriptome profiling and phenotyping of rootstock and scion in a tomato/potato heterografting system

Zhang

Mao

Wang

et al. 2018

Physiologia Plantarum

View full text Add to dashboard Cite

show abstract

“…The grafted plants were obtained by cutting them into a wedge shape and inserting them into a "V"-shaped incision in the rootstock (Peres et al, 2005). A completely randomized design was used with four treatments from combinations between MT and au (MT/au, au/MT, MT/MT and au/au, the first genotype being the graft and the second the rootstock) with three repetitions.…”

Section: Grafting and Cultivationmentioning

confidence: 99%

Root-to-Shoot Communication to Modulate Source-Sink Relationship in Tomato Depends on Phytochromes

Galati¹,

Oliveira²,

Gaion³

et al. 2018

JAS

View full text Add to dashboard Cite

Phytochromes have been reported as strategic photoreceptors that can modulate the partition of photoassimilates between source and sink. However, so far, it is unknown whether phytochrome accumulation in the root is part of the control mechanisms of the source-sink relationship that modulates root and shoot growth. Thus, the objective of this work was to investigate phytochrome involvement in the source-sink relationship and in the vegetative and reproductive development of tomato plants (Solanum lycopersicum L. cv. Micro-Tom or MT). The experimental design was completely randomized with four treatments, provided by grafting combinations between aurea (au), which is phytochrome deficient, and the near isogenic line MT: (MT/MT, au/au, MT/au and au/MT). We observed differentiated responses for many parameters analyzed. For example, the root dry mass accumulation and stern diameter obtained by MT/MT, MT/au and au/MT grafting were 33% and 31% higher, respectively, than those obtained by au/au. In the au/MT combination, there were greater root dry mass and total dry mass accumulations. Based on the changes in vegetative and reproductive development observed from grafting combinations between MT and the mutant au, we can conclude that phytochromes function in the control of photoassimilate partitioning between roots and stems during tomato growth.

show abstract

“…Grafting of tomato mutants onto potato stocks has been proposed as a strategy to elucidate the role of hormones in long-distance signaling, although the results so far point to effects on assimilate distribution rather than on signaling pathways (Peres et al, 2005). …”

Section: Sucrose and Other Putative Long-range Signaling Moleculesmentioning

confidence: 99%

A critical appraisal of phloem-mobile signals involved in tuber induction

Suárez‐López¹

2013

Front. Plant Sci.

View full text Add to dashboard Cite

The identification of FLOWERING LOCUS T (FT) and several FT homologs as phloem-mobile proteins that regulate flowering has sparked the search for additional homologs involved in the long-distance regulation of other developmental processes. Given that flowering and tuber induction share regulatory pathways, the quest for long-distance tuberization signals has been further stimulated. Several tuberization regulators have been proposed as mobile molecules, including the FT family protein StSP6A, the plant growth regulators gibberellins and the microRNA miR172. Although some of these hypotheses are attractive and plausible, evidence that these molecules are transmissible in potato has yet to be obtained. Two mRNAs encoding transcription factors, StBEL5 and POTATO HOMEOBOX 1 (POTH1), are mobile and correlate with tuber induction. However, evidence that StBEL5 or POTH1 are required for tuberization is not available yet. Therefore, there are several good candidates for long-distance molecules in the tuberization process. Further research should test their role as systemic tuberization signals.

show abstract

Grafting of tomato mutants onto potato rootstocks: An approach to study leaf-derived signaling on tuberization

Cited by 32 publications

References 40 publications

Comprehensive transcriptome profiling and phenotyping of rootstock and scion in a tomato/potato heterografting system

Comprehensive transcriptome profiling and phenotyping of rootstock and scion in a tomato/potato heterografting system

Root-to-Shoot Communication to Modulate Source-Sink Relationship in Tomato Depends on Phytochromes

A critical appraisal of phloem-mobile signals involved in tuber induction

Contact Info

Product

Resources

About