Grain dust induces IL-8 production from bronchial epithelial cells: effect on neutrophil recruitment

Park, Hae‐Sim; Suh, Jung-Hee; Kim, Sun-Sin; Kwon, O Jung

doi:10.1016/s1081-1206(10)62414-3

Cited by 6 publications

(6 citation statements)

References 16 publications

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“…Mechanisms of this neutrophilic inflammation are unclear, but it may be due to an irritant effect of agents in the workplace, as happens with many stimuli, such as repeated inhalation of hypertonic saline (24) or atmospheric pollutants, such as ozone (25). Exposure to swine confinement (26), or grain dust (27) also induce a neutrophilic inflammation. Some occupational agents may also induce asthma-like symptoms and neutrophilic inflammation through the same mechanism.…”

Section: Discussionmentioning

confidence: 99%

An Effective Strategy for Diagnosing Occupational Asthma

Girard

Chaboillez

Cartier

et al. 2004

Am J Respir Crit Care Med

114

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Monitoring airway inflammation by means of induced sputum cell counts seems to improve the management of asthma. We sought to assess whether such monitoring at the end of periods at and away from work combined with the monitoring of PEF could improve the diagnosis of occupational asthma. We enrolled subjects suspected of having occupational asthma. Serial monitoring of PEF was performed during 2 weeks at and away from work. At the end of each period, induced sputum was collected. Specific inhalation challenge was subsequently performed. PEF graphs were interpreted visually by five independent observers. Forty-nine subjects, including 23 with positive specific inhalation challenge, completed the study. The addition of sputum cell counts to the monitoring of PEF increased the specificity of this test, respectively, by 18 (range [r] 13.7-25.5) or 26.8% (r 24.8-30.4) depending if an increase of sputum eosinophils greater than 1 or 2% when at work was considered as significant. The sensitivity increased by 8.2% (r 4.1-13.4) or decreased by 12.3% (r 3.1-24.1) depending on the cutoff value in sputum eosinophils chosen (greater than 1 or 2%, respectively). The addition of sputum cell counts to PEF monitoring is useful to improve the diagnosis of occupational asthma.

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Section: Discussionmentioning

confidence: 99%

An Effective Strategy for Diagnosing Occupational Asthma

Girard

Chaboillez

Cartier

et al. 2004

Am J Respir Crit Care Med

114

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“…The optimal concentration of wheat antigens to stimulate HAECs was chosen based on the previous study and preliminary experiments 14 . Wheat exposure increased the release of TGF-β1 and eotaxin-2 as well as IL-8 in a dose-dependent manner ( P < 0.05, Fig.…”

Section: Resultsmentioning

confidence: 99%

“…In addition, wheat contains bacterial endotoxins and lipopolysaccharides that induce innate immune responses involved in the pathogenesis of asthma 11, 12. Wheat exposure could induce release of interleukin (IL)-8 from HAECs that enhance neutrophil migration and activation in collaboration with myeloperoxidase (MPO) levels 13, 14. Increased level of S100A8 and folliculin from HAECs and mannose-binding lectin are involved in innate immune responses in bakery workers,15, 16, 17 where eosinophil activation and their interaction with inflammatory cells have not been established yet.…”

Section: Introductionmentioning

confidence: 99%

Transforming growth factor-β1 and eosinophil-derived neurotoxins contribute to the development of work-related respiratory symptoms in bakery workers

Trinh

Ulambayar

Cao

et al. 2019

World Allergy Organization Journal

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Background: In baker's asthma previous studies suggest that adaptive and innate immunity are involved in the development of work-related respiratory symptoms (WRS), where we hypothesized that epithelial cells derive airway inflammation through modulating the release of inflammatory cytokines. Thus, we conducted this study to investigate the role of epithelial cell-derived cytokines in the development of WRS among bakery workers. Methods: We recruited 385 wheat-exposed subjects with WRS (WRSþ)/without WRS (WRS-) working in a single industry and 243 unexposed controls from Ajou Medical Center (Suwon, South Korea). Levels of epithelial cell-derived cytokines (interleukin [IL-8], transforming growth factor-b1 [TGF-b1], eotaxin-2) and inflammatory mediators (eosinophil-derived neurotoxins [EDN]) in sera or cell-free supernatants were measured by ELISA. Human airway epithelial cells (HAECs), A549, were stimulated by wheat flour extracts and co-cultured with peripheral blood neutrophils isolated from 4 asthmatic patients. Results: Serum TGF-b1 levels were significantly lower in exposed subjects than in unexposed controls, in the WRSþ group than in the WRS-group (P < 0.001 for each). The WRSþ group had a significantly higher level of serum EDN than the WRS-group (P < 0.001). Serum TGF-b1 and EDN levels predicted the development of WRS in exposed subjects (area under the curve [AUC] ¼ 0.719, 72.4% sensitivity/70% specificity; AUC ¼ 0.759, 78.6% sensitivity/60% specificity). From wheat-stimulated HAECs, TGF-b1 release peaked at 6 hours after wheat exposure, while eotaxin-2 peaked at 12 hours. Co-culture of HAECs with neutrophils did not affect TGF-b1 release. Conclusions: Our results suggest that TGF-b1 may contribute to develop type-2 airway inflammation and WRS. Serum TGF-b1/EDN levels may be potential serum biomarkers for predicting WRS among bakery workers.

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“…The suppressive effect was presented as % inhibition calculated as follows: (100-[IL-8 level preincubated with blocking antibody or dexamethasone/IL-8 level preincubated without blocking antibody or dexamethasone]) ×100. The IL-8 level was quantified using a homemade sandwich-type ELISA as described in our previous report (10). This ELISA had less than 10% of intra-and inter-assay coefficient variation.…”

Section: Experimental Designmentioning

confidence: 99%

Exposure to Toluene Diisocyanate (TDI) Induces IL-8 Production from Bronchial Epithelial Cells: Effect of Pro-inflammatory Cytokines

et al. 2003

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This investigation was designed to confirm IL-8 production from human bronchial epithelial cells with toluene diisocyanate (TDI) exposure and to examine the effects of pro-inflammatory cytokine and dexamethasone. We cultured Beas-2B, a bronchial epithelial cell line with TDI-HSA conjugate and compared with those without conjugate. IL-8 in the supernatant was measured by ELISA. To evaluate the effect of proinflammatory cytokines, peripheral blood mononuclear cells (PBMC) were collected from TDI- and non-TDI asthma patients, and were added to the epithelial cell culture. Dexamethasone or antibodies to TNF-alpha and IL-1beta were pre-incubated with PBMC supernatant. There was a significant production of IL-8 from bronchial epithelial cells with addition of TDI-HSA conjugate in a dose-dependent manner, which was significantly augmented with addition of PBMC supernatant. Higher production of IL-8 was noted with addition of PBMC supernatant from TDI-asthma patients than in those from non-TDI asthma patients. IL-1beta and IL-1beta/TNF alpha antibodies were able to suppress the IL-8 productions. Pre-treatment of dexamethasone induced dose-dependent inhibition of the IL-8 production. These results suggest that the IL-8 production from bronchial epithelial cells contribute to neutrophil recruitment occurring in TDI-induced airway inflammation. IL-1beta released from PBMC of TDI-induced asthma patients may be one of the pro-inflammatory cytokines to enhance IL-8 production.

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Grain dust induces IL-8 production from bronchial epithelial cells: effect on neutrophil recruitment

Cited by 6 publications

References 16 publications

An Effective Strategy for Diagnosing Occupational Asthma

An Effective Strategy for Diagnosing Occupational Asthma

Transforming growth factor-β1 and eosinophil-derived neurotoxins contribute to the development of work-related respiratory symptoms in bakery workers

Exposure to Toluene Diisocyanate (TDI) Induces IL-8 Production from Bronchial Epithelial Cells: Effect of Pro-inflammatory Cytokines

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