Wheat is one of the major world agricultural products, extensively grown worldwide due to the unique dough rheological properties and the bread-baking quality of its flour (Arzani & Ashraf, 2017). According to EUROSTAT (2020), in 2019, the harvested production of cereals in the EU-27 was 299.3 million tons from which common wheat and spelt account for 131.8 million tons or 44% of total cereals production.Cultivated area of common wheat and spelt in EU in 2019 was 24.2 million ha (EUROSTAT, 2020). On the other hand, the production of the ancient wheat species, such as, diploid einkorn (Triticum monococcum L.), tetraploid emmer (T. dicoccum L.), and hexaploid spelt (T. spelta L.) is suppressed (Geisslitz et al., 2018). In Serbia, spelt is cultivated on the area of 157.9 ha, mostly in the northern region called Vojvodina (120.04 ha) (Vojnov et al., 2020), while emmer and khorasan are produced in a significantly smaller scale compared to spelt. The main reason behind this is low yield (Geisslitz et al., 2018), difficulties related to the processing of grains, as well as inferior technological quality (Arzani & Ashraf, 2017) compared to the common wheat. Regardless of these shortcomings, ancient wheat varieties are considered environment friendly because of its disease resistance and low fertilizer input. Moreover, considering high adaptability to the altered climatic conditions, ancient wheat represents a valuable genetic resource of interest to breeders (Boukid et al., 2018).