Gram-negative bacteria carrying β-lactamase encoding genes in hospital and urban wastewater in Brazil

Zagui, Guilherme Sgobbi; Andrade, Leonardo Neves; Moreira, Natália Columbaro; Silva, Thaís Vilela; Machado, Gabriel Pinheiro; Darini, Ana Lúcia da Costa

doi:10.1007/s10661-020-08319-w

Cited by 26 publications

(17 citation statements)

References 42 publications

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“…Researchers commonly describe hospital wastewater as a source of a wide spectrum of antibiotic-resistant bacteria and antibiotic resistance genes (Chagas et al, 2011;Korzeniewska and Harnisz, 2013;Hocquet et al, 2016;Rowe et al, 2017). The literature also identified a greater amount of drug resistance genes in wastewater originating from hospitals than in exclusively municipal wastewater by comparing the occurrence of betalactam antibiotic resistance genes (Zagui et al, 2020). The genes occurring most abundantly in samples from both treatment plants included bla TEM and bla OXA .…”

Section: Discussionmentioning

confidence: 99%

Impact of Hospital Wastewater on the Occurrence and Diversity of Beta-Lactamase Genes During Wastewater Treatment with an Emphasis on Carbapenemase Genes: A Metagenomic Approach

Hubeny

Ciesielski

Harnisz

et al. 2021

Front. Environ. Sci.

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The diversity of beta-lactam antibiotic resistance genes, with particular emphasis on carbapenemase genes, during the treatment process at two wastewater treatment plants (WWTPs) with different levels of hospital wastewater inflow was investigated using high-throughput sequencing. An additional aspect of the study was to determine the taxonomic diversity of microorganisms in the studied samples. The obtained results suggest that bacteria of the Fusobacteriaceae family, not associated to date with this phenomenon, may be involved in the spread of antibiotic resistance in the environment. In samples from both wastewater treatment plants, the dominant beta-lactamase genes included blaOXA, blaGES, blaBEL, blaCfxA, and blaTEM. It is worth noting that the blaKPC and blaNDM genes were only found in untreated municipal wastewater with a higher hospital wastewater content. Moreover, an increase in the abundance of the blaIMP gene after the biological treatment stage in the studied treatment plants was found. In wastewater characterized by a higher proportion of hospital wastewater, 94 correlations were observed, while in wastewater with its lower proportion, 41 correlations were noted. Considering the above, the current research indicates that the inflow of hospital wastewater contributes to the spread of antibiotic resistance in the aquatic environment.

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Section: Discussionmentioning

confidence: 99%

Impact of Hospital Wastewater on the Occurrence and Diversity of Beta-Lactamase Genes During Wastewater Treatment with an Emphasis on Carbapenemase Genes: A Metagenomic Approach

Hubeny

Ciesielski

Harnisz

et al. 2021

Front. Environ. Sci.

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“…AMR analysis supported these findings, with detection of MDR, CARB-R and ESC-R profiles and BL genes (such as bla KPC-like , bla OXA-23-like , bla IMP-like , bla SHV-like bla CTX-M-1 , bla CTX-M-8 , and bla CTX-M-9 ) in all of these contamination hotspots. Several studies conducted in Brazil and Latin America have confirmed the relevance of these BL genes in the AMR scenario (Jácome et al, 2016;Labarca et al, 2016;Rocha et al, 2016Rocha et al, , 2019da Silva et al, 2019;Romanin et al, 2019;Zagui et al, 2020). The oligotypes tracking allowed us to verify that, in general, the bacteria found in patients sites are more related to each other (different body sites) than to their surrounding environment.…”

Section: Discussionmentioning

confidence: 76%

Healthcare-Associated Infections-Related Bacteriome and Antimicrobial Resistance Profiling: Assessing Contamination Hotspots in a Developing Country Public Hospital

Sereia

Christoff²,

Cruz³

et al. 2021

Front. Microbiol.

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Hospital-built environment colonization by healthcare-associated infections-related bacteria (HAIrB) and the interaction with their occupants have been studied to support more effective tools for HAI control. To investigate HAIrB dynamics and antimicrobial resistance (AMR) profile we carried out a 6-month surveillance program in a developing country public hospital, targeting patients, hospital environment, and healthcare workers, using culture-dependent and culture-independent 16S rRNA gene sequencing methods. The bacterial abundance in both approaches shows that the HAIrB group has important representativeness, with the taxa Enterobacteriaceae, Pseudomonas, Staphylococcus, E. coli, and A. baumannii widely dispersed and abundant over the time at the five different hospital units included in the survey. We observed a high abundance of HAIrB in the patient rectum, hands, and nasal sites. In the healthcare workers, the HAIrB distribution was similar for the hands, protective clothing, and mobile phones. In the hospital environment, the healthcare workers resting areas, bathrooms, and bed equipment presented a wide distribution of HAIrB and AMR, being classified as contamination hotspots. AMR is highest in patients, followed by the environment and healthcare workers. The most frequently detected beta-lactamases genes were, blaSHV–like, blaOXA–23–like, blaOXA–51–like, blaKPC–like, blaCTX–M–1, blaCTX–M–8, and blaCTX–M–9 groups. Our results demonstrate that there is a wide spread of antimicrobial resistance due to HAIrB in the hospital environment, circulating among patients and healthcare workers. The contamination hotspots identified proved to be constant over time. In the fight for patient safety, these findings can reorient practices and help to set up new guidelines for HAI control.

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“…Resistance to several antibiotics (ESBL, 3rd generation cepholosporins, tetracyclines, quinolones and carbapenems) were reported by Picão et al, 2013 [60]. Wastewaters were studied intensely in South America [50,55,57,72]. Irrigation water has also been linked to the dissemination of AMR [82,84].…”

Section: Discussionmentioning

confidence: 99%

Anthropogenic Activities and the Problem of Antibiotic Resistance in Latin America: A Water Issue

Domı́nguez

Chacón

Wallace

2021

Water

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Antibiotics revolutionized modern medicine and have been an excellent tool to fight infections. However, their overuse and misuse in different human activities such as health care, food production and agriculture has resulted in a global antimicrobial resistance crisis. Some regions such as Latin America present a more complex scenario because of the lack of resources, systematic studies and legislation to control the use of antimicrobials, thus increasing the spread of antibiotic resistance. This review aims to summarize the state of environmental antibiotic resistance in Latin America, focusing on water resources. Three databases were searched to identify publications on antimicrobial resistance and anthropogenic activities in relation to natural and artificial water ecosystems. We found that antibiotic resistant bacteria, mainly against beta lactam antibiotics, have been reported in several Latin American countries, and that resistant bacteria as well as resistant genes can be isolated from a wide variety of aquatic environments, including drinking, surface, irrigation, sea and wastewater. It is urgent to establish policies and regulations for antibiotic use to prevent the increase of multi-drug resistant microorganisms in the environment.

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Gram-negative bacteria carrying β-lactamase encoding genes in hospital and urban wastewater in Brazil

Cited by 26 publications

References 42 publications

Impact of Hospital Wastewater on the Occurrence and Diversity of Beta-Lactamase Genes During Wastewater Treatment with an Emphasis on Carbapenemase Genes: A Metagenomic Approach

Impact of Hospital Wastewater on the Occurrence and Diversity of Beta-Lactamase Genes During Wastewater Treatment with an Emphasis on Carbapenemase Genes: A Metagenomic Approach

Healthcare-Associated Infections-Related Bacteriome and Antimicrobial Resistance Profiling: Assessing Contamination Hotspots in a Developing Country Public Hospital

Anthropogenic Activities and the Problem of Antibiotic Resistance in Latin America: A Water Issue

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