Gram-negative bacterial lipid A analysis by negative electrospray ion trap mass spectrometry: Stepwise dissociations of deprotonated species under low energy CID conditions

Structural analysis of the lipid A isolated from Hafnia alvei 32 and PCM 1192 lipopolysaccharides

“…4B ). The frequency of the loss of phosphoric acid from the position O-1 was signifi cantly higher than from O-4' during ESI-MS 2 analysis due to the lability of glycosidically linked P group ( 28,31 ). Interpretation of the observed fragment ions is presented in Fig.…”

“…I). Because the obtained data suggested the E. coli -type structure of studied lipid A, ESI-MS n spectra were compared with data published previously for lipid A of E. coli (29)(30)(31).…”

Characterization of Lipid A Variants by Energy-Resolved Mass Spectrometry: Impact of Acyl Chains

“…As shown previously, UVPD results in structure-specific glycosidic and cross-ring fragments of lipid A and affords richer fragmentation patterns when compared to conventional collisional activation methods [27–35]. Multi-stage MS n strategies have also been important for structural characterization of lipid A [36–38]. Recent work by the Goodlett group successfully determined the structures of various lipid A using an automated hierarchical tandem MS algorithm known as HitMS [37, 38].…”

Collision induced dissociation-based characterization of nucleotide peptides: Fragmentation patterns of microcin C7–C51, an antimicrobial peptide produced by Escherichia coli

“…Three competing fragmentation pathways are thus proposed for MccC7-C51*: MccC7-C51 and MccC7-C51* show that, for such even-electron complex systems, the isomerization into ion-dipole complexes takes place before dissociation. This behavior is well known for a large class of chemical compounds including organic and biological molecules, such as certain amino steroids [12], glycoconjugates [14], steroid fatty acids [26], DNA adduct steroids [13], lipopolysaccharides [27], and peptides [28]. If the complex life time is sufficiently large, due to the relative low internal energy compared to the dissociation energy threshold, internal proton transfer can occur, resulting from the formation of complementary ion pairs.…”