Gram negative organisms in community acquired respiratory tract infections

Shah, Manoj

doi:10.5799/ahinjs.02.2013.01.0071

Cited by 3 publications

(1 citation statement)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Escherichia coli is generally a large and diverse bacterium that colonises both human and animal intestine as well as being found in the environment. This bacterium ranges from being harmless to pathogenic, a leading cause of urinary tract infections, respiratory infections [6][7][8], pneumonia [9], global food poisoning [10][11][12]. In all cases of pathogenic E. coli infections resistance to drugs of choice have been previously reported [13] with isolates exhibiting multi-drug resistance (MDR) a view that had also been expressed in an earlier report [14].…”

Section: Biomedical Research 2018; 29 (17): 3303-3310mentioning

confidence: 99%

Antibiotic susceptibility and plasmid profile of clinical isolates of Escherichia coli

Badger-Emeka¹,

Ineta²,

Emeka³

et al. 2018

biomedicalresearch

View full text Add to dashboard Cite

Background/Aims: The role of mobile genetic elements such as plasmids in the spread of multiple antibiotic resistance has continued to receive attention by researchers. Due to the diverse nature of Escherichia coli strains characteristically harbouring large proportion of genomic plasmids, the aim of the present study is to evaluate the antimicrobial susceptibility and plasmid profiles of clinical isolates of E. coli in this south eastern region of Saudi Arabia. Methods: Bacterial isolation, antimicrobial susceptibility test were carried out using basic microbiological techniques. Dendrogram of clustering in relationship to antibiotic susceptibility of the isolates was carried out using PAST. Extracted plasmid DNA was visualised using the ultraviolet light illuminator and photo documentation system. Sizes of Plasmid were determined using standard DNA molecular weight markers at 1kb ladder. Plasmid curing was carried out using Sodium Dodecyl Sulphate (SDS). Results and conclusion: Antibiotic resistance ranged between 85.71%-100% for the cephalosporins, 64.29% to 92.9% resistance for penicillins, including Aztreonam, a last line drug. Results indicate that 70% of the isolates did not show any plasmid presence. However, 30% of the isolates contained plasmids with molecular weights of 30kb and above. Dendrogram of clustering in relationship to antibiotic susceptibility showed isolates were in 3 clades pre-plasmid curing and 6 clades post-plasmid curing. One isolate (EC36) was cured of its resistance to Penicillins, while isolates EC67, EC77 and EC87 initially sensitive to ciprofloxacin, became resistant to the antibiotic. The findings suggest a preponderance of chromosomal mediated resistant E. coli. The few plasmid containing resistant bacteria, were resistant to curing as they displayed continued antibiotic resistance after curing. This therefore confirms the growing presence of multiple genomic plasmid harbouring E. coli.

show abstract

Section: Biomedical Research 2018; 29 (17): 3303-3310mentioning

confidence: 99%

Antibiotic susceptibility and plasmid profile of clinical isolates of Escherichia coli

Badger-Emeka¹,

Ineta²,

Emeka³

et al. 2018

biomedicalresearch

View full text Add to dashboard Cite

show abstract

Lower Respiratory Tract Pathogens and Their Antimicrobial Susceptibility Pattern: A 5-Year Study

et al. 2021

View full text Add to dashboard Cite

Lower respiratory tract infections (LRTIs) are the most common infections in humans. It is estimated that 2.74 million deaths worldwide occur each year due to LRTIs. The aim of the study was to determine the frequency and antibiotic susceptibility pattern of microorganisms isolated from respiratory samples of patients with LRTIs. Between January 2015 and December 2019, a total of 7038 sputum and bronchoaspirate samples from suspected LRTI patients were collected. Among them, 2753 samples (39.1%) showed significant microbial growth on culture media. The LRTI rate was higher in patients with male gender (67.1%) and with age between 40–59 years (48.6%). The microorganism identification and antibiotic susceptibility testing were performed with Vitek 2. Out of 4278 isolates species, 3102 (72.5%) were Gram-negative bacteria, 1048 (24.5%) were Gram-positive bacteria, and 128 (3.0%) were Candida spp. Major microorganisms isolated were Acinetobacter baumannii (18.6%), Staphylococcus aureus (15.2%), Pseudomonas aeruginosa (14.2%), and Klebsiella pneumoniae (10.9%). In antimicrobial susceptibility testing, Staphylococcus aureus isolates were mostly resistant to Penicillin G (84.1%) and Oxacillin (48.1%), whereas they demonstrated maximum sensitivity to Tigecycline (100%) and Linezolid (99.5%). Among Gram-negative isolates, Acinetobacter baumannii showed maximum sensitivity to Colistin but was resistant to other antibiotics (95–99%). Klebsiella pneumoniae isolates were mostly resistant to Cefotaxime (72.7%) and sensitive to Gentamicin (54.3%), and Pseudomonas aeruginosa was resistant to Ciprofloxacin (40.3%) and sensitive to Amikacin (85.9%). Gram-negative bacteria represented the species most commonly isolated. A high rate of antimicrobial resistance was observed in this study. In conclusion, the correct identification of causative microorganisms and their susceptibility patterns to antibiotics is crucial for choosing targeted and effective antibiotic therapy in LRTIs, and to prevent the emergence of multidrug-resistant bacteria.

show abstract

Evaluating Local Susceptibility Profile of Bacterial Isolates to Various Antibiotics Using Syndromic Antibiogram: A Cross-sectional Study

Dhivya,

Aswini,

Hanusha

et al. 2024

Asian Journal of Pharmaceutical Research and Health Care

View full text Add to dashboard Cite

Background: A traditional antibiogram is a summary of the overall susceptibility profile of a bacterial isolate to a variety of antibiotics however it lacks the inclusion of the site of infection and hospital location. Hence, this study focuses on the importance of incorporating a syndromic antibiogram (SA) which provides an increased likelihood of appropriate empiric antibiotic therapy for a specific infectious syndrome and stratifying the susceptibilities based on patient location in providing effective antibiotic therapy. Objectives: The objective is to assess the local susceptibility profile of bacterial isolates to different antibiotics using SA in a tertiary care hospital. Methodology: A cross-sectional study was carried out in a tertiary care hospital over 6 months. A total of 400 samples were collected, out of which 350 samples were included based on inclusion criteria. The SA for urinary tract infection (UTI), respiratory tract infection (RTI), and bloodstream infection (BSI) was prepared. The collected data were analyzed using Microsoft Excel 2019 and SPSS version 24. The Chi-square test was used to find out the association between the isolates, patient location, and the syndromes. Continuous data were presented as mean ± standard deviation. Categorical data were presented as frequency and percentage. Results: Three hundred and fifty positive culture reports were included in our analysis, and the majority of isolates were Gram-negative bacilli rather than Gram-positive Cocci. Escherichia coli, streptococci, and Salmonella typhi were the most predominant organisms found in UTI, RTI, and BSI. E. coli showed a high level of susceptibility to fosfomycin (99%) and amikacin (99%). Streptococci showed a high susceptibility to linezolid (97%) and penicillin (94%) and S. typhi showed resistance to ciprofloxacin (0%). Conclusion: This study clearly depicted the variations in isolated microorganism’s susceptibility rate and their resistance pattern in specific units of the hospital. Incorporation of SA provides better guidance for the clinician in selecting the most appropriate empiric therapy for individual patients.

show abstract

Gram negative organisms in community acquired respiratory tract infections

Cited by 3 publications

References 9 publications

Antibiotic susceptibility and plasmid profile of clinical isolates of Escherichia coli

Antibiotic susceptibility and plasmid profile of clinical isolates of Escherichia coli

Lower Respiratory Tract Pathogens and Their Antimicrobial Susceptibility Pattern: A 5-Year Study

Evaluating Local Susceptibility Profile of Bacterial Isolates to Various Antibiotics Using Syndromic Antibiogram: A Cross-sectional Study

Contact Info

Product

Resources

About