Gram‐Positive Bacteria Cell Wall Driven Self‐Disassembled Nanovesicles against Methicillin‐Resistant <i>Staphylococcus Aureus</i>

An, Hong‐Wei; Fei, Yue; Yan, Tong‐Da; Lu, Chu‐Qi; Wang, Man‐Di; Ma, Teng; Zhao, Bo‐Yan; Nie, J.; Tseng, Hsian-Rong; Li, Lili; Wang, Hao

doi:10.1002/adtp.201900217

Cited by 4 publications

(2 citation statements)

References 38 publications

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“…As a biosurfactant with a macrolide structure, it was difficult for LSL to interact with the cell wall of S. aureus . In addition, LSL might mainly promote the formation of biosurfactant-enriched domains within the phospholipid bilayer and inhibit the protein synthesis function of the cell membrane ( Kaneko et al, 2007 ; An et al, 2020 ; Marcelino et al, 2021 ). But for P. aeruginosa , the peptidoglycan layer of the cell wall is thin, the cross-linking is loose, and the lipid content is high ( Rajivgandhi et al, 2018 ).…”

Section: Discussionmentioning

confidence: 99%

Comparison of inhibitory effects and mechanisms of lactonic sophorolipid on different pathogenic bacteria

Wang

Zhang

et al. 2022

Front. Microbiol.

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Crude sophorolipids (SLs) have been proven to perform varying degrees of inhibitory effects on different pathogenic bacteria. However, systematic comparative studies of pure lactonic sophorolipid (LSL) among different types of bacteria are few. In this study, the antibacterial effects and mechanisms of LSL on pathogenic bacteria of Staphylococcus aureus, Lactobacillus sp., Pseudomonas aeruginosa, and Escherichia coli were investigated. Bacteriostatic circle, antibacterial rate, minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC) of LSL on different pathogenic bacteria were measured. Then, the antibacterial mechanisms of LSL on S. aureus and P. aeruginosa were explored using ultrastructural observation, cell membrane permeability analysis, intracellular ATP content determination, and extracellular UV absorption detection. With the minimum MIC and MBC values of 0.05 and 0.20 mg/ml, LSL exhibited the best inhibitory effect against S. aureus, followed by P. aeruginosa. LSL showed no significant inhibitory effect on E. coli and Lactobacillus sp. For both S. aureus and P. aeruginosa, LSL achieved bacteriostatic and bactericidal effects by destroying the cell wall, increasing the permeability of the cell membrane and leading to the flow out of intracellular contents. However, the action mode and action intensity of LSL on the cell wall and membrane of these two bacteria were significantly different. LSL had a greater influence on the cell membrane of S. aureus by “leaking,” while it exhibited a stronger effect on the cell wall of P. aeruginosa by “blasting.” These results contributed to a better understanding of the relationship between LSL and different bacterial cell structures, further suggesting the conclusion that LSL might be used for the targeted treatment of special pathogenic bacteria.

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Section: Discussionmentioning

confidence: 99%

Comparison of inhibitory effects and mechanisms of lactonic sophorolipid on different pathogenic bacteria

Wang

Zhang

et al. 2022

Front. Microbiol.

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“…To characterize the morphology of peptide nanofibers, TEM was used to observe PBS (0.01 mol/L,pH = 7.4) solution. 47 Peptide solutions were dissolved in PBS to a prepared final concentration of 128 μM PCBP and placed on 100-mesh copper-plated grids for permeabilization. After 3 min, the excess solution was wiped off and the samples were air-dried for 30 min.…”

Section: Tem Observation Of Peptide Nanofibersmentioning

confidence: 99%

Design of High-Selectivity Co-Assembled Peptide Nanofibers against Bacterial Infection in Piglets

Tan

Tang

et al. 2023

ACS Appl. Mater. Interfaces

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Antibiotic resistance is an escalating global health concern that could result in tens of millions of deaths annually from drug-resistant bacterial infections in the future, especially in animal husbandry. Peptide antibacterial nanomaterials offer a competitive alternative to antibiotics because of their distinct mechanism of physically penetrating pathogenic biological membranes. This study developed amphiphilic co-assembled peptide nanofibers with high biological selectivity (PCBP-NCAP NFs) to overcome the high cytotoxicity of peptide PCBP and the low antibacterial activity of peptide NCAP. PCBP-NCAP NFs exhibit broad-spectrum antibacterial activity and excellent biocompatibility, with negligible in vivo and in vitro toxicity. Additionally, PCBP-NCAP NFs possess direct antibacterial efficacy and potential immunomodulatory capabilities using a piglet systemic infection model. Its unique mechanism of membrane penetration and the ability to bind to anionic components on the surface of pathogenic bacteria make them less susceptible to drug resistance. In conclusion, these findings have significant implications for the advancement of supramolecular peptide nanomedicines for clinical application and animal husbandry.

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Designing Self‐Assembling Chimeric Peptide Nanoparticles with High Stability for Combating Piglet Bacterial Infections

Tan

Tang

et al. 2022

Advanced Science

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As a novel type of antibiotic alternative, peptide‐based antibacterial drug shows potential application prospects attributable to their unique mechanism for lysing the membrane of pathogenic bacteria. However, peptide‐based antibacterial drugs suffer from a series of problems, most notably their immature stability, which seriously hinders their application. In this study, self‐assembling chimeric peptide nanoparticles (which offer excellent stability in the presence of proteases and salts) are constructed and applied to the treatment of bacterial infections. In vitro studies are used to demonstrate that peptide nanoparticles NPs1 and NPs2 offer broad‐spectrum antibacterial activity and desirable biocompatibility, and they retain their antibacterial ability in physiological salt environments. Peptide nanoparticles NPs1 and NPs2 can resist degradation under high concentrations of proteases. In vivo studies illustrate that the toxicity caused by peptide nanoparticles NPs1 and NPs2 is negligible, and these nanoparticles can alleviate systemic bacterial infections in mice and piglets. The membrane permeation mechanism and interference with the cell cycle differ from that of antibiotics and mean that the nanoparticles are at a lower risk of inducing drug resistance. Collectively, these advances may accelerate the development of peptide‐based antibacterial nanomaterials and can be applied to the construction of supramolecular nanomaterials.

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Gram‐Positive Bacteria Cell Wall Driven Self‐Disassembled Nanovesicles against Methicillin‐Resistant Staphylococcus Aureus

Cited by 4 publications

References 38 publications

Comparison of inhibitory effects and mechanisms of lactonic sophorolipid on different pathogenic bacteria

Comparison of inhibitory effects and mechanisms of lactonic sophorolipid on different pathogenic bacteria

Design of High-Selectivity Co-Assembled Peptide Nanofibers against Bacterial Infection in Piglets

Designing Self‐Assembling Chimeric Peptide Nanoparticles with High Stability for Combating Piglet Bacterial Infections

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