Gramella antarctica sp. nov., isolated from marine surface sediment

Abstract

A Gram-stain-negative, aerobic, yellow-coloured, motile by gliding, rod-shaped bacterial strain, designated R17H11, was isolated from surface sediment collected from the Ross Sea, Antarctica. Growth optimally occurred at 25-30 °C, at pH 7.0-7.5 and in the presence of 3 % NaCl (w/v). Phylogenetic trees based on 16S rRNA gene sequences indicated that strain R17H11 clustered together with Gramella flava JLT2011 and fell within the genus Gramella. Strain R17H11 shared the highest 16S rRNA gene similarities (96.1 and 96.0 %) with the type strains of Gramella forsetii and G. flava, and 92.6-95.5 % similarities with those of other known Gramella species. Strain R17H11 contained menaquinone-6 as the only isoprenoid quinone. The major fatty acids (>5 %) were summed feature 3 (17.5 %, comprising C16 : 1ω7c and/or C16 : 1ω6c), iso-C15 : 0 (14.0 %), summed feature 9 (11.8 %, comprising 10-methyl C16 : 0 and/or iso-C17 : 1ω9c), iso-C17 : 0 3-OH (11.8 %), iso-C16 : 0 (7.4 %), C17 : 1ω6c (6.9 %) and anteiso-C15 : 0 (5.1 %). The major polar lipids were phosphatidylethanolamine, four unidentified lipids, an unidentified aminolipid, an unidentified aminophospholipid and an unidentified glycolipid. The DNA G+C content of strain R17H11 was 38.6 mol%. On the basis of the phylogenetic, physiological and chemotaxonomic characteristics, strain R17H11 represents a novel species in the genus Gramella, for which the name Gramellaantarctica sp. nov. is proposed. The type strain of the novel species is R17H11 (=GDMCC 1.1208=KCTC 52925).

Gramella sediminis sp. nov., isolated from a tidal flat of the Yellow Sea

“…Moreover, strain ASW11-100 T contained less summed feature 3 (10.3 %) than detected in G. forsetii (14.9%). The sole respiratory quinone detected in ASW11-100 T was MK-6, which was consistent with those of all species of the genus Gramella [12][13][14][15][16][17][18][19][20][21][22][23][24][25][26][27][28][29][30].…”

“…forsetii (14.9%). The sole respiratory quinone detected in ASW11-100 T was MK-6, which was consistent with those of all species of the genus Gramella [12–30]. The polar lipid profile (Fig.…”

Mesonia oceanica sp. nov., isolated from oceans during the Tara oceans expedition, with a preference for mesopelagic waters

“… *, Data from [38] †, Data from [39] ‡, Data from [40] §, Determined by HPLC (all other values, from WGS). …”

Culture-Dependent and -Independent Analyses Reveal the Diversity, Structure, and Assembly Mechanism of Benthic Bacterial Community in the Ross Sea, Antarctica

“…In the present study, spread-plate method was used as parallel complement of culture-independent approach and yielded 629 isolates, which would be helpful to perform more metabolic, physiological and ecological tests in future. Our previous studies have phenotypically, phylogenetically and chemotaxonomically characterized five novel type strains out of the 629 isolates (Li et al, 2017a,b, 2018a,b,c).…”

“…Two gram of each sediment stored at 4°C was measured out with a stainless steel spoon and subsequently resuspended in 20 mL sterile seawater. The suspension were serially diluted and spread on Marine Agar 2216 (MA, BD Difco, MD, USA), MC (Li et al, 2017a,b, 2018a,b,c) and LC (1/100 MC) plates. The suspension plating process was performed in parallel to generate two analogous groups of plates, which were incubated at 4°C for 30 days and at 25°C for 7 days, respectively.…”