Granin proteins (chromogranin A and secretogranin II C23-3 and C26-3) in the endocrine pancreas of reptiles

Trandaburu, Tiberiu; Ali, S. Syed; Trandaburu, Ioana

doi:10.1016/s0940-9602(99)80032-6

Cited by 7 publications

(9 citation statements)

References 20 publications

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“…In addition, no CG A-IR cells were demonstrated in the pancreas of red-eared slider (Ku et al, 2000). In the present study, somewhat differed from previous studies (Trandaburu et al, 1999;Ku et al, 2000), bCG-IR cells were detected in the pancreatic islets and exocrine of this grass lizard. However, it is considered that single use of bCG is not suitable as a marker of endocrine cells in the pancreas of this grass lizard because a density of bCG-IR cells was lower than that of insulinand glucagon-IR cells.…”

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confidence: 99%

“…In addition, peptide tyrosine tyrosine-and neuropeptide tyrosine-(Della Rossa and Putti, 1995) and chromogranin (CG)- (Trandaburu et al, 1999) IR cells were found in the lacertid lizard pancreas and new types of endocrine cells have been reported in the pancreas of the various vertebrates.…”

Section: Original Papermentioning

confidence: 99%

“…CGs have been found to occur in large variety of endocrine organs and cells outside the adrenal medulla, and they have been claimed as common "markers" of all neuroendocrine cells (Cohn et al, 1984). In addition, Trandaburu et al (1999) reported that CG A-IR cells were found only in the turtle pancreas among the four reptile species belonging to the turtles, lizards and snakes whereas secretogranin II-IR cells appeared both in the turtle and snake. In addition, no CG A-IR cells were demonstrated in the pancreas of red-eared slider (Ku et al, 2000).…”

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confidence: 99%

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The distribution and frequency of endocrine cells in the splenic lobe of grass lizard (Takydromus wolteri): An immunohistochemical study

Hs²

2009

Eur J Histochem

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The regional distribution and frequency of the pancreatic endocrine cells in the splenic lobe of grass lizard, Takydromus wolteri, were studied by immunohistochemical (PAP) method using six types of specific mammalian antisera against bovine Sp-1/chromogranin (bCG), serotonin, insulin, glucagon, somatostatin and human pancreatic polypeptide (hPP). The pancreas was subdivided into two regions -islet kike and exocrine regions. The frequency of each immunoreactive (IR) endocrine cells was calculated as mean number/total 100 islet cells and as mean number/total 1000 cells (including exocrine and endocrine cells) using automated image analysis process. In addition, the percentage of each IR cell was also calculated. All of six endocrine cells were demonstrated. They were dispersed in the whole pancreatic parenchyma between exocrine acinar cells, or they were also observed as islet like clusters. In islet-like regions, bCG-, insulin-and glucagon-IR cells were detected as one or two cell layer cords and they were located between this cell-cords with 14.30±5.62, 61.50±9.76 and 26.50±9.31/100 cells frequencies, respectively. However, somatostatin-IR cells were mainly located in the peripheral parts not in cell-cords with 12.40±4.86/100 cells, and no serotonin-and hPP-IR cells were demonstrated. In exocrine regions, all of bCG-, serotonin-, insulin-, glucagon-, somatostatin-and hPP-IR cells were detected and they occurred mainly among the exocrine parenchyma as solitary cells with 10. 30±2.54, 0.80±0.63, 15.50±5.30, 5.80±2.66, 3.10±1.29 and 11.00±3.33/1000 cells frequencies, respectively. In addition, serotonin-IR cells were mainly located between epithelia and connective tissue of pancreatic duct. Overall, there were 0.58±0.49% serotonin-, 56.44±9.35% insulin-, 23.73±8.22% glucagon-, 11.28±3.03% somatostatin-and 7.97±2.02% hPP-IR cells

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The distribution and frequency of endocrine cells in the splenic lobe of grass lizard (Takydromus wolteri): An immunohistochemical study

Hs²

2009

Eur J Histochem

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“…This is actually not surprising because the precursor protein SgII, as well as secretogranin III, another protein belonging to the chromogranin family, has already been reported to occur in the islets of Langerhans, which is the endocrine part of the pancreas Trandaburu et al 1999;Sakai et al 2004). SgII also shares the characteristics of manserin in that it does not occur in the exocrine part of the pancreas (Fischer-Colbrie et al 1995).…”

Section: Discussionmentioning

confidence: 95%

“…There are reports that not only SgII, SN and EM66, which are excision products from SgII, are localized in pancreas (FischerColbrie et al 1995;Leitner et al 1996;Trandaburu et al 1999;Stridsberg et al 2008). Therefore, it is conceivable that manserin occurs in the pancreas and may exert physiological functions in the endocrine system.…”

Section: Introductionmentioning

confidence: 99%

Manserin, a secretogranin II-derived peptide, distributes in the rat endocrine pancreas colocalized with islet-cell specific manner

Tano

Oyabu

Tashiro

et al. 2010

Histochem Cell Biol

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Manserin is a recently characterized 40-amino acid neuropeptide derived from secretogranin II, a protein belonging to the chromogranin family. Although the physiological roles of manserin have not been elucidated to date, manserin has been shown to distribute in not only the brain but also the endocrine system such as the pituitary and adrenal glands, suggesting its role in the endocrine system. The present study aimed to explore the occurrence and distribution of manserin in the rat pancreas using an immunohistochemical technique with a polyclonal antibody against rat manserin. Immunoreactivity for manserin was readily detected in almost whole islets of Langerhans whereas not at all in the exocrine pancreas. Manserin-expressing cells were not colocalized with the glucagon-secreting cells (alpha cells), whereas they colocalized with insulin-secreting cells (beta cells) and somatostatin-secreting cells (delta cells), although their intracellular distribution was different. These results indicate that manserin, occurring in the endocrine pancreas, may have a potential role in the endocrine system.

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Histochemistry and cell biology: the annual review 2010

Hübner

Efthymiadis

2011

Histochem Cell Biol

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This review summarizes recent advances in histochemistry and cell biology which complement and extend our knowledge regarding various aspects of protein functions, cell and tissue biology, employing appropriate in vivo model systems in conjunction with established and novel approaches. In this context several non-expected results and discoveries were obtained which paved the way of research into new directions. Once the reader embarks on reading this review, it quickly becomes quite obvious that the studies contribute not only to a better understanding of fundamental biological processes but also provide use-oriented aspects that can be derived therefrom.

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Granin proteins (chromogranin A and secretogranin II C23-3 and C26-3) in the endocrine pancreas of reptiles

Cited by 7 publications

References 20 publications

The distribution and frequency of endocrine cells in the splenic lobe of grass lizard (Takydromus wolteri): An immunohistochemical study

The distribution and frequency of endocrine cells in the splenic lobe of grass lizard (Takydromus wolteri): An immunohistochemical study

Manserin, a secretogranin II-derived peptide, distributes in the rat endocrine pancreas colocalized with islet-cell specific manner

Histochemistry and cell biology: the annual review 2010

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